Optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of Centella asiatica (L.) as a medicinal herb.
The present study investigates the effects of different concentrations, as well as type of plant growth regulators (PGRs) and medium (MS, Duchefa) on the growth and development of Centella asiatica in semi-solid culture. In addition, a protocol for successful sterilization of C.asiatica explants pr...
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| Format: | Article |
| Language: | English English |
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MDPI
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24605/ http://psasir.upm.edu.my/id/eprint/24605/1/Optimization%20of%20an%20efficient%20semi.pdf |
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| author | Moghaddam, Sina Siavash Jaafar, Hawa Abdul Aziz, Maheran Ibrahim, Rusli Rahmat, Asmah Philip, Elizabeth |
| author_facet | Moghaddam, Sina Siavash Jaafar, Hawa Abdul Aziz, Maheran Ibrahim, Rusli Rahmat, Asmah Philip, Elizabeth |
| author_sort | Moghaddam, Sina Siavash |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The present study investigates the effects of different concentrations, as well as type of plant growth regulators (PGRs) and medium (MS, Duchefa) on the growth and
development of Centella asiatica in semi-solid culture. In addition, a protocol for successful sterilization of C.asiatica explants prepared from field-grown plants highly
exposed to fungal and bacterial contamination was determined. Results for sterilization treatments revealed that applying HgCl2 and Plant Preservative Mixture (PPM) with cetrimide, bavistin and trimethoprim which were included after washing with tap water, followed by the addition of PPM in the medium, produced a very satisfactory result (clean culture 90 ± 1.33%) and TS5 (decon + cetrimide 1% + bavistin 150 mg/L + trimethoprim
50 mg/L + HgCl2 0.1% + PPM 2% soak and 2 mL/L in medium) was hence chosen as the best method of sterilization for C.asiatica. The synergistic combination of 6 benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) in concentrations of 2 mg/L and 0.1 mg/L, respectively, in Duchefa medium compared with MS induced the most optimal percentage of sprouted shoots (93 ± 0.667), number of shoots (5.2 ± 0.079) and nodes (4 ± 0.067) per explant, leaf per explant (14 ± 0.107) and shoot lengt (4.1 ± 0.67 cm). Furthermore, optimum rooting frequency (95.2 ± 0.81%), the number of roots/shoot (7.5 ± 0.107) and the mean root length (4.5 ± 0.133 cm) occurred for shoots that were cultured on full-strength MS medium containing 0.5 mg/L indole-3-butyric acid (IBA). In this study, the acclimatized plantlets were successfully established with almost 85% survival. The findings of this study have proven an efficient medium and PGR concentration for the mass propagation of C.asiatica. These findings would be useful in
micropropagation and ex situ conservation of this plant. |
| first_indexed | 2025-11-15T08:41:10Z |
| format | Article |
| id | upm-24605 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:41:10Z |
| publishDate | 2011 |
| publisher | MDPI |
| recordtype | eprints |
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| spelling | upm-246052015-10-16T06:29:59Z http://psasir.upm.edu.my/id/eprint/24605/ Optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of Centella asiatica (L.) as a medicinal herb. Moghaddam, Sina Siavash Jaafar, Hawa Abdul Aziz, Maheran Ibrahim, Rusli Rahmat, Asmah Philip, Elizabeth The present study investigates the effects of different concentrations, as well as type of plant growth regulators (PGRs) and medium (MS, Duchefa) on the growth and development of Centella asiatica in semi-solid culture. In addition, a protocol for successful sterilization of C.asiatica explants prepared from field-grown plants highly exposed to fungal and bacterial contamination was determined. Results for sterilization treatments revealed that applying HgCl2 and Plant Preservative Mixture (PPM) with cetrimide, bavistin and trimethoprim which were included after washing with tap water, followed by the addition of PPM in the medium, produced a very satisfactory result (clean culture 90 ± 1.33%) and TS5 (decon + cetrimide 1% + bavistin 150 mg/L + trimethoprim 50 mg/L + HgCl2 0.1% + PPM 2% soak and 2 mL/L in medium) was hence chosen as the best method of sterilization for C.asiatica. The synergistic combination of 6 benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) in concentrations of 2 mg/L and 0.1 mg/L, respectively, in Duchefa medium compared with MS induced the most optimal percentage of sprouted shoots (93 ± 0.667), number of shoots (5.2 ± 0.079) and nodes (4 ± 0.067) per explant, leaf per explant (14 ± 0.107) and shoot lengt (4.1 ± 0.67 cm). Furthermore, optimum rooting frequency (95.2 ± 0.81%), the number of roots/shoot (7.5 ± 0.107) and the mean root length (4.5 ± 0.133 cm) occurred for shoots that were cultured on full-strength MS medium containing 0.5 mg/L indole-3-butyric acid (IBA). In this study, the acclimatized plantlets were successfully established with almost 85% survival. The findings of this study have proven an efficient medium and PGR concentration for the mass propagation of C.asiatica. These findings would be useful in micropropagation and ex situ conservation of this plant. MDPI 2011-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24605/1/Optimization%20of%20an%20efficient%20semi.pdf Moghaddam, Sina Siavash and Jaafar, Hawa and Abdul Aziz, Maheran and Ibrahim, Rusli and Rahmat, Asmah and Philip, Elizabeth (2011) Optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of Centella asiatica (L.) as a medicinal herb. Molecules, 16 (-). pp. 8981-8991. ISSN 1420-3049; ESSN:1420-3049 http://www.mdpi.com/ 10.3390/molecules16118981 English |
| spellingShingle | Moghaddam, Sina Siavash Jaafar, Hawa Abdul Aziz, Maheran Ibrahim, Rusli Rahmat, Asmah Philip, Elizabeth Optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of Centella asiatica (L.) as a medicinal herb. |
| title | Optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of Centella asiatica (L.) as a medicinal herb. |
| title_full | Optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of Centella asiatica (L.) as a medicinal herb. |
| title_fullStr | Optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of Centella asiatica (L.) as a medicinal herb. |
| title_full_unstemmed | Optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of Centella asiatica (L.) as a medicinal herb. |
| title_short | Optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of Centella asiatica (L.) as a medicinal herb. |
| title_sort | optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of centella asiatica (l.) as a medicinal herb. |
| url | http://psasir.upm.edu.my/id/eprint/24605/ http://psasir.upm.edu.my/id/eprint/24605/ http://psasir.upm.edu.my/id/eprint/24605/ http://psasir.upm.edu.my/id/eprint/24605/1/Optimization%20of%20an%20efficient%20semi.pdf |