Inhibitory effects of palm a-, c- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in BV2 microglia.

Anti-inflammatory actions of the vitamin E fragment tocotrienol have not been described for microglia. Here, we screened palm α-, γ- and δ-tocotrienol isoforms and Tocomin® 50% (contains spectrum of tocotrienols and tocopherols) for their ability to limit nitric oxide (NO) production by BV2 microgli...

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Main Authors: Tan, Shi Wei, Ramasamy, Rajesh, Abdullah, Maha, Vidyadaran, Sharmili
Format: Article
Language:English
English
Published: Elsevier 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24566/
http://psasir.upm.edu.my/id/eprint/24566/1/Inhibitory%20effects%20of%20palm%20a.pdf
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author Tan, Shi Wei
Ramasamy, Rajesh
Abdullah, Maha
Vidyadaran, Sharmili
author_facet Tan, Shi Wei
Ramasamy, Rajesh
Abdullah, Maha
Vidyadaran, Sharmili
author_sort Tan, Shi Wei
building UPM Institutional Repository
collection Online Access
description Anti-inflammatory actions of the vitamin E fragment tocotrienol have not been described for microglia. Here, we screened palm α-, γ- and δ-tocotrienol isoforms and Tocomin® 50% (contains spectrum of tocotrienols and tocopherols) for their ability to limit nitric oxide (NO) production by BV2 microglia. Microglia were treated with varying doses of tocotrienols for 24h and stimulated with 1 μg/ml lipopolysaccharide (LPS). All tocotrienol isoforms reduced NO release by LPS-stimulated microglia, with 50 μM being the most potent tocotrienol dose. Of the isoforms tested, δ-tocotrienol lowered NO levels the most, reducing NO by approximately 50% at 48 h post-LPS treatment (p<.05). None of the tocotrienol doses tested affected microglia viability.
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language English
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last_indexed 2025-11-15T08:40:59Z
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spelling upm-245662016-01-12T04:32:32Z http://psasir.upm.edu.my/id/eprint/24566/ Inhibitory effects of palm a-, c- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in BV2 microglia. Tan, Shi Wei Ramasamy, Rajesh Abdullah, Maha Vidyadaran, Sharmili Anti-inflammatory actions of the vitamin E fragment tocotrienol have not been described for microglia. Here, we screened palm α-, γ- and δ-tocotrienol isoforms and Tocomin® 50% (contains spectrum of tocotrienols and tocopherols) for their ability to limit nitric oxide (NO) production by BV2 microglia. Microglia were treated with varying doses of tocotrienols for 24h and stimulated with 1 μg/ml lipopolysaccharide (LPS). All tocotrienol isoforms reduced NO release by LPS-stimulated microglia, with 50 μM being the most potent tocotrienol dose. Of the isoforms tested, δ-tocotrienol lowered NO levels the most, reducing NO by approximately 50% at 48 h post-LPS treatment (p<.05). None of the tocotrienol doses tested affected microglia viability. Elsevier 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24566/1/Inhibitory%20effects%20of%20palm%20a.pdf Tan, Shi Wei and Ramasamy, Rajesh and Abdullah, Maha and Vidyadaran, Sharmili (2011) Inhibitory effects of palm a-, c- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in BV2 microglia. Cellular Immunology, 271 (2011). pp. 205-209. ISSN 0008-8749; ESSN: 1090-2163 10.1016/j.cellimm.2011.07.012 English
spellingShingle Tan, Shi Wei
Ramasamy, Rajesh
Abdullah, Maha
Vidyadaran, Sharmili
Inhibitory effects of palm a-, c- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in BV2 microglia.
title Inhibitory effects of palm a-, c- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in BV2 microglia.
title_full Inhibitory effects of palm a-, c- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in BV2 microglia.
title_fullStr Inhibitory effects of palm a-, c- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in BV2 microglia.
title_full_unstemmed Inhibitory effects of palm a-, c- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in BV2 microglia.
title_short Inhibitory effects of palm a-, c- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in BV2 microglia.
title_sort inhibitory effects of palm a-, c- and δ-tocotrienol on lipopolysaccharide-induced nitric oxide production in bv2 microglia.
url http://psasir.upm.edu.my/id/eprint/24566/
http://psasir.upm.edu.my/id/eprint/24566/
http://psasir.upm.edu.my/id/eprint/24566/1/Inhibitory%20effects%20of%20palm%20a.pdf