Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia.
The usefulness of mec-associated dru typing in the epidemiological analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Malaysia was investigated and compared with pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and spa and SCCmec typing. The isolate...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Springer Verlag
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24564/ http://psasir.upm.edu.my/id/eprint/24564/1/Mec.pdf |
| _version_ | 1848845070796587008 |
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| author | Ghaznavi-Rad, Ehsanollah Goering, R. V. Shamsudin, Mariana Nor Weng, Poh Leng Sekawi, Zamberi Tavakol, M. Van Belkum, Alex Neela, Vasantha Kumari |
| author_facet | Ghaznavi-Rad, Ehsanollah Goering, R. V. Shamsudin, Mariana Nor Weng, Poh Leng Sekawi, Zamberi Tavakol, M. Van Belkum, Alex Neela, Vasantha Kumari |
| author_sort | Ghaznavi-Rad, Ehsanollah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The usefulness of mec-associated dru typing in the epidemiological analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Malaysia was investigated and compared with pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and spa and SCCmec typing. The isolates studied included all MRSA types in Malaysia. Multilocus sequence type ST188 and ST1 isolates were highly clonal by all typing methods. However, the dru typing of ST239 isolates produced the clearest discrimination between SCCmec IIIa and III isolates, yielding more subtypes than any other method. Evaluation of the discriminatory power for each method identified dru typing and PFGE as the most discriminatory, with Simpson’s index of diversity (SID) values over 89%, including an isolate which was non-typeable by spa, but dru-typed as dt13j. The discriminatory ability of dru typing, especially with closely related MRSA ST239 strains (e.g., Brazilian and Hungarian), underscores its utility as a tool for the epidemiological investigation of MRSA. |
| first_indexed | 2025-11-15T08:40:58Z |
| format | Article |
| id | upm-24564 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:40:58Z |
| publishDate | 2011 |
| publisher | Springer Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-245642015-11-26T08:42:14Z http://psasir.upm.edu.my/id/eprint/24564/ Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia. Ghaznavi-Rad, Ehsanollah Goering, R. V. Shamsudin, Mariana Nor Weng, Poh Leng Sekawi, Zamberi Tavakol, M. Van Belkum, Alex Neela, Vasantha Kumari The usefulness of mec-associated dru typing in the epidemiological analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Malaysia was investigated and compared with pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and spa and SCCmec typing. The isolates studied included all MRSA types in Malaysia. Multilocus sequence type ST188 and ST1 isolates were highly clonal by all typing methods. However, the dru typing of ST239 isolates produced the clearest discrimination between SCCmec IIIa and III isolates, yielding more subtypes than any other method. Evaluation of the discriminatory power for each method identified dru typing and PFGE as the most discriminatory, with Simpson’s index of diversity (SID) values over 89%, including an isolate which was non-typeable by spa, but dru-typed as dt13j. The discriminatory ability of dru typing, especially with closely related MRSA ST239 strains (e.g., Brazilian and Hungarian), underscores its utility as a tool for the epidemiological investigation of MRSA. Springer Verlag 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24564/1/Mec.pdf Ghaznavi-Rad, Ehsanollah and Goering, R. V. and Shamsudin, Mariana Nor and Weng, Poh Leng and Sekawi, Zamberi and Tavakol, M. and Van Belkum, Alex and Neela, Vasantha Kumari (2011) Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia. European Journal of Clinical Microbiology & Infectious Diseases, 30 (11). pp. 1365-1369. ISSN 0934-9723; ESSN: 1435-4373 10.1007/s10096-011-1230-1 English |
| spellingShingle | Ghaznavi-Rad, Ehsanollah Goering, R. V. Shamsudin, Mariana Nor Weng, Poh Leng Sekawi, Zamberi Tavakol, M. Van Belkum, Alex Neela, Vasantha Kumari Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia. |
| title | Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia. |
| title_full | Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia. |
| title_fullStr | Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia. |
| title_full_unstemmed | Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia. |
| title_short | Mec-associated dru typing in the epidemiological analysis of ST239 MRSA in Malaysia. |
| title_sort | mec-associated dru typing in the epidemiological analysis of st239 mrsa in malaysia. |
| url | http://psasir.upm.edu.my/id/eprint/24564/ http://psasir.upm.edu.my/id/eprint/24564/ http://psasir.upm.edu.my/id/eprint/24564/1/Mec.pdf |