Possible participation of nitric oxide/cyclic guanosine monophosphate/protein kinase C/ATP-sensitive K + channels pathway in the systemic antinociception of flavokawin B.

The possible mechanisms of action in the antinociceptive activity induced by systemic administration (intraperitoneal, i.p.) of flavokawin B (FKB) were analysed using chemical models of nociception in mice. It was demonstrated that i.p. administration of FKB to the mice at 0.3, 1.0, 3.0 and 10 mg/k...

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Main Authors: Tengku Mohamad, Tengku Azam Shah, Akhtar, Muhammad Nadeem, Khalivulla, Shaik Ibrahim, Perimal, Enoch Kumar, Khalid, Mohamed Hanief, Hui, Ming Ong, Zareen, Seema, Omar Farouk, Ahmad Akira, Israf Ali, Daud Ahmad, Lajis, Md. Nordin, Sulaiman, Mohd Roslan
Format: Article
Language:English
English
Published: Wiley 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24557/
http://psasir.upm.edu.my/id/eprint/24557/1/Possible%20participation%20of%20nitric%20oxide.pdf
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author Tengku Mohamad, Tengku Azam Shah
Akhtar, Muhammad Nadeem
Khalivulla, Shaik Ibrahim
Perimal, Enoch Kumar
Khalid, Mohamed Hanief
Hui, Ming Ong
Zareen, Seema
Omar Farouk, Ahmad Akira
Israf Ali, Daud Ahmad
Lajis, Md. Nordin
Sulaiman, Mohd Roslan
author_facet Tengku Mohamad, Tengku Azam Shah
Akhtar, Muhammad Nadeem
Khalivulla, Shaik Ibrahim
Perimal, Enoch Kumar
Khalid, Mohamed Hanief
Hui, Ming Ong
Zareen, Seema
Omar Farouk, Ahmad Akira
Israf Ali, Daud Ahmad
Lajis, Md. Nordin
Sulaiman, Mohd Roslan
author_sort Tengku Mohamad, Tengku Azam Shah
building UPM Institutional Repository
collection Online Access
description The possible mechanisms of action in the antinociceptive activity induced by systemic administration (intraperitoneal, i.p.) of flavokawin B (FKB) were analysed using chemical models of nociception in mice. It was demonstrated that i.p. administration of FKB to the mice at 0.3, 1.0, 3.0 and 10 mg/kg produced significant dose-related reduction in the number of abdominal constrictions. The antinociception induced by FKB in the acetic acid test was significantly attenuated by i.p. pre-treatment of mice with l-arginine, the substrate for nitric oxide synthase or glibenclamide, the ATP-sensitive K+ channel inhibitor, but was enhanced by methylene blue, the non-specific guanylyl cyclase inhibitor. FKB also produced dose-dependent inhibition of licking response caused by intraplantar injection of phorbol 12-myristate 13-acetate, a protein kinase C activator (PKC). Together, these data indicate that the NO/cyclic guanosine monophosphate/PKC/ATP-sensitive K+ channel pathway possibly participated in the antinociceptive action induced by FKB.
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spelling upm-245572016-01-12T04:36:56Z http://psasir.upm.edu.my/id/eprint/24557/ Possible participation of nitric oxide/cyclic guanosine monophosphate/protein kinase C/ATP-sensitive K + channels pathway in the systemic antinociception of flavokawin B. Tengku Mohamad, Tengku Azam Shah Akhtar, Muhammad Nadeem Khalivulla, Shaik Ibrahim Perimal, Enoch Kumar Khalid, Mohamed Hanief Hui, Ming Ong Zareen, Seema Omar Farouk, Ahmad Akira Israf Ali, Daud Ahmad Lajis, Md. Nordin Sulaiman, Mohd Roslan The possible mechanisms of action in the antinociceptive activity induced by systemic administration (intraperitoneal, i.p.) of flavokawin B (FKB) were analysed using chemical models of nociception in mice. It was demonstrated that i.p. administration of FKB to the mice at 0.3, 1.0, 3.0 and 10 mg/kg produced significant dose-related reduction in the number of abdominal constrictions. The antinociception induced by FKB in the acetic acid test was significantly attenuated by i.p. pre-treatment of mice with l-arginine, the substrate for nitric oxide synthase or glibenclamide, the ATP-sensitive K+ channel inhibitor, but was enhanced by methylene blue, the non-specific guanylyl cyclase inhibitor. FKB also produced dose-dependent inhibition of licking response caused by intraplantar injection of phorbol 12-myristate 13-acetate, a protein kinase C activator (PKC). Together, these data indicate that the NO/cyclic guanosine monophosphate/PKC/ATP-sensitive K+ channel pathway possibly participated in the antinociceptive action induced by FKB. Wiley 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24557/1/Possible%20participation%20of%20nitric%20oxide.pdf Tengku Mohamad, Tengku Azam Shah and Akhtar, Muhammad Nadeem and Khalivulla, Shaik Ibrahim and Perimal, Enoch Kumar and Khalid, Mohamed Hanief and Hui, Ming Ong and Zareen, Seema and Omar Farouk, Ahmad Akira and Israf Ali, Daud Ahmad and Lajis, Md. Nordin and Sulaiman, Mohd Roslan (2011) Possible participation of nitric oxide/cyclic guanosine monophosphate/protein kinase C/ATP-sensitive K + channels pathway in the systemic antinociception of flavokawin B. Basic & Clinical Pharmacology & Toxicology, 108 (6). pp. 400-405. ISSN 1742-7851; ESSN: 1742-7843 10.1111/j.1742-7843.2010.00670.x English
spellingShingle Tengku Mohamad, Tengku Azam Shah
Akhtar, Muhammad Nadeem
Khalivulla, Shaik Ibrahim
Perimal, Enoch Kumar
Khalid, Mohamed Hanief
Hui, Ming Ong
Zareen, Seema
Omar Farouk, Ahmad Akira
Israf Ali, Daud Ahmad
Lajis, Md. Nordin
Sulaiman, Mohd Roslan
Possible participation of nitric oxide/cyclic guanosine monophosphate/protein kinase C/ATP-sensitive K + channels pathway in the systemic antinociception of flavokawin B.
title Possible participation of nitric oxide/cyclic guanosine monophosphate/protein kinase C/ATP-sensitive K + channels pathway in the systemic antinociception of flavokawin B.
title_full Possible participation of nitric oxide/cyclic guanosine monophosphate/protein kinase C/ATP-sensitive K + channels pathway in the systemic antinociception of flavokawin B.
title_fullStr Possible participation of nitric oxide/cyclic guanosine monophosphate/protein kinase C/ATP-sensitive K + channels pathway in the systemic antinociception of flavokawin B.
title_full_unstemmed Possible participation of nitric oxide/cyclic guanosine monophosphate/protein kinase C/ATP-sensitive K + channels pathway in the systemic antinociception of flavokawin B.
title_short Possible participation of nitric oxide/cyclic guanosine monophosphate/protein kinase C/ATP-sensitive K + channels pathway in the systemic antinociception of flavokawin B.
title_sort possible participation of nitric oxide/cyclic guanosine monophosphate/protein kinase c/atp-sensitive k + channels pathway in the systemic antinociception of flavokawin b.
url http://psasir.upm.edu.my/id/eprint/24557/
http://psasir.upm.edu.my/id/eprint/24557/
http://psasir.upm.edu.my/id/eprint/24557/1/Possible%20participation%20of%20nitric%20oxide.pdf