Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats.

The immunomodulatory activity of Cassia auriculata (CA)-derived polyphenols was tested on aged rats. Rats (24–26 months old) were given CA polyphenols supplementation at doses of 25, 50, and 100 mg/kg for 28 days. Flow cytometry analysis of CA polyphenols-treated aged rats showed increased T and B c...

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Main Authors: John, Cini M., Sandrasaigaran, Pratheep, Chih, Kong Tong, Adam, Aishah, Ramasamy, Rajesh
Format: Article
Language:English
English
Published: Elsevier 2011
Online Access:http://psasir.upm.edu.my/id/eprint/24534/
http://psasir.upm.edu.my/id/eprint/24534/1/Immunomodulatory%20activity%20of%20polyphenols%20derived%20from%20Cassia%20auriculata%20flowers%20in%20aged%20rats.pdf
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author John, Cini M.
Sandrasaigaran, Pratheep
Chih, Kong Tong
Adam, Aishah
Ramasamy, Rajesh
author_facet John, Cini M.
Sandrasaigaran, Pratheep
Chih, Kong Tong
Adam, Aishah
Ramasamy, Rajesh
author_sort John, Cini M.
building UPM Institutional Repository
collection Online Access
description The immunomodulatory activity of Cassia auriculata (CA)-derived polyphenols was tested on aged rats. Rats (24–26 months old) were given CA polyphenols supplementation at doses of 25, 50, and 100 mg/kg for 28 days. Flow cytometry analysis of CA polyphenols-treated aged rats showed increased T and B cells percentage along with enhanced proliferation of splenocytes in both resting and LPS-stimulated cells. Increased percentage of pan T cells is further supported by an elevation of CD4+, CD8+, and CD4+CD25+ regulatory cells. In terms of innate immune cell activity, CA polyphenol supplementation reduced the oxidative burst activity of neutrophils in response to PMA and Escherichia coli activation. Our results collectively show that polyphenols derived from CA boost T cell immunity by increasing the number of T cells and its sensitivity towards stimulants and decreasing ROS production by neutrophils that could potentially harm multiple biological systems in aged individuals.
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publishDate 2011
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spelling upm-245342015-10-07T01:23:00Z http://psasir.upm.edu.my/id/eprint/24534/ Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats. John, Cini M. Sandrasaigaran, Pratheep Chih, Kong Tong Adam, Aishah Ramasamy, Rajesh The immunomodulatory activity of Cassia auriculata (CA)-derived polyphenols was tested on aged rats. Rats (24–26 months old) were given CA polyphenols supplementation at doses of 25, 50, and 100 mg/kg for 28 days. Flow cytometry analysis of CA polyphenols-treated aged rats showed increased T and B cells percentage along with enhanced proliferation of splenocytes in both resting and LPS-stimulated cells. Increased percentage of pan T cells is further supported by an elevation of CD4+, CD8+, and CD4+CD25+ regulatory cells. In terms of innate immune cell activity, CA polyphenol supplementation reduced the oxidative burst activity of neutrophils in response to PMA and Escherichia coli activation. Our results collectively show that polyphenols derived from CA boost T cell immunity by increasing the number of T cells and its sensitivity towards stimulants and decreasing ROS production by neutrophils that could potentially harm multiple biological systems in aged individuals. Elsevier 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24534/1/Immunomodulatory%20activity%20of%20polyphenols%20derived%20from%20Cassia%20auriculata%20flowers%20in%20aged%20rats.pdf John, Cini M. and Sandrasaigaran, Pratheep and Chih, Kong Tong and Adam, Aishah and Ramasamy, Rajesh (2011) Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats. Cellular Immunology, 271 (2). pp. 474-479. ISSN 0008-8749; ESSN: 1090-2163 10.1016/j.cellimm.2011.08.017 English
spellingShingle John, Cini M.
Sandrasaigaran, Pratheep
Chih, Kong Tong
Adam, Aishah
Ramasamy, Rajesh
Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats.
title Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats.
title_full Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats.
title_fullStr Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats.
title_full_unstemmed Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats.
title_short Immunomodulatory activity of polyphenols derived from Cassia auriculata flowers in aged rats.
title_sort immunomodulatory activity of polyphenols derived from cassia auriculata flowers in aged rats.
url http://psasir.upm.edu.my/id/eprint/24534/
http://psasir.upm.edu.my/id/eprint/24534/
http://psasir.upm.edu.my/id/eprint/24534/1/Immunomodulatory%20activity%20of%20polyphenols%20derived%20from%20Cassia%20auriculata%20flowers%20in%20aged%20rats.pdf