Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice.
OBJECTIVE: Azadirachta indica (Neem) has been used traditionally for many centuries. Some impressive therapeutic qualities have been discovered. However, the therapeutic effect of neem leaf extract in 4T1 breast cancer has not been documented. The purpose of the present study is to investigate the...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24519/ |
| _version_ | 1848845059078750208 |
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| author | Othman, Fauziah Motalleb, Gholamreza Lam, Sally Tsuey Rahmat, Asmah Fakurazi, Sharida Chong, Pei Pei |
| author_facet | Othman, Fauziah Motalleb, Gholamreza Lam, Sally Tsuey Rahmat, Asmah Fakurazi, Sharida Chong, Pei Pei |
| author_sort | Othman, Fauziah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | OBJECTIVE:
Azadirachta indica (Neem) has been used traditionally for many centuries. Some impressive therapeutic qualities have been discovered. However, the therapeutic effect of neem leaf extract in 4T1 breast cancer has not been documented. The purpose of the present study is to investigate the therapeutic effect of ethanolic Neem leaf extract in an in vivo 4T1 breast cancer model in mice.
MATERIALS AND METHODS:
A total of 84 female BALB/c mice were divided randomly into 7 groups (3 non-cancerous groups and 4 cancerous groups) consisting of 12 mice per group. The 3 non-cancerous groups were normal mice treated with 0.5% of Tween 20 in phosphate buffer saline (PBS) (NC), 250 mg/kg Neem (N250) or 500 mg/kg Neem (N500). The 4 cancerous groups were; cancer controls treated with 0.5% of Tween 20 in PBS (CC), and cancerous mice treated with 0.5 µg/mL tamoxifen citrate (CT), 250 mg/kg Neem leaf extract (CN 250) or 500 mg/kg Neem leaf extract (CN 500). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were used to evaluate apoptosis (cell death) in the breast cancer tissues. SPSS software, version 14 was used for statistical analysis. Statistical significance was defined as p≤0.05. Non parametric analysis of variance (ANOVA) was performed with the Kruskal Wallis test for the TUNEL assays. Parametric data among the groups was compared using ANOVA.
RESULTS:
TUNEL assays showed that the CN 250 and CN 500 groups had a higher incidence of apoptosis compared with the cancer controls.
CONCLUSION:
The findings showed that neem leaf extract induces apoptosis in 4T1 breast cancer BALB/c mice. |
| first_indexed | 2025-11-15T08:40:47Z |
| format | Article |
| id | upm-24519 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:40:47Z |
| publishDate | 2011 |
| publisher | Royan Institute (ACECR), Tehran |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-245192014-03-24T07:21:34Z http://psasir.upm.edu.my/id/eprint/24519/ Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice. Othman, Fauziah Motalleb, Gholamreza Lam, Sally Tsuey Rahmat, Asmah Fakurazi, Sharida Chong, Pei Pei OBJECTIVE: Azadirachta indica (Neem) has been used traditionally for many centuries. Some impressive therapeutic qualities have been discovered. However, the therapeutic effect of neem leaf extract in 4T1 breast cancer has not been documented. The purpose of the present study is to investigate the therapeutic effect of ethanolic Neem leaf extract in an in vivo 4T1 breast cancer model in mice. MATERIALS AND METHODS: A total of 84 female BALB/c mice were divided randomly into 7 groups (3 non-cancerous groups and 4 cancerous groups) consisting of 12 mice per group. The 3 non-cancerous groups were normal mice treated with 0.5% of Tween 20 in phosphate buffer saline (PBS) (NC), 250 mg/kg Neem (N250) or 500 mg/kg Neem (N500). The 4 cancerous groups were; cancer controls treated with 0.5% of Tween 20 in PBS (CC), and cancerous mice treated with 0.5 µg/mL tamoxifen citrate (CT), 250 mg/kg Neem leaf extract (CN 250) or 500 mg/kg Neem leaf extract (CN 500). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were used to evaluate apoptosis (cell death) in the breast cancer tissues. SPSS software, version 14 was used for statistical analysis. Statistical significance was defined as p≤0.05. Non parametric analysis of variance (ANOVA) was performed with the Kruskal Wallis test for the TUNEL assays. Parametric data among the groups was compared using ANOVA. RESULTS: TUNEL assays showed that the CN 250 and CN 500 groups had a higher incidence of apoptosis compared with the cancer controls. CONCLUSION: The findings showed that neem leaf extract induces apoptosis in 4T1 breast cancer BALB/c mice. Royan Institute (ACECR), Tehran 2011 Article PeerReviewed Othman, Fauziah and Motalleb, Gholamreza and Lam, Sally Tsuey and Rahmat, Asmah and Fakurazi, Sharida and Chong, Pei Pei (2011) Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice. Cell Journal, 13 (2). pp. 107-116. ISSN 2228-5806; ESSN: 2228-5814 English |
| spellingShingle | Othman, Fauziah Motalleb, Gholamreza Lam, Sally Tsuey Rahmat, Asmah Fakurazi, Sharida Chong, Pei Pei Extract of Azadirachta indica (Neem) leaf induces apoptosis in 4T1 breast cancer BALB/c mice. |
| title | Extract of Azadirachta indica (Neem) leaf induces
apoptosis in 4T1 breast cancer BALB/c mice. |
| title_full | Extract of Azadirachta indica (Neem) leaf induces
apoptosis in 4T1 breast cancer BALB/c mice. |
| title_fullStr | Extract of Azadirachta indica (Neem) leaf induces
apoptosis in 4T1 breast cancer BALB/c mice. |
| title_full_unstemmed | Extract of Azadirachta indica (Neem) leaf induces
apoptosis in 4T1 breast cancer BALB/c mice. |
| title_short | Extract of Azadirachta indica (Neem) leaf induces
apoptosis in 4T1 breast cancer BALB/c mice. |
| title_sort | extract of azadirachta indica (neem) leaf induces
apoptosis in 4t1 breast cancer balb/c mice. |
| url | http://psasir.upm.edu.my/id/eprint/24519/ |