Purification, characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of Mangifera pajang fruits.
Polysaccharides were isolated from the fibrous pulp of bambangan (Mangifera pajang Kort.). Neutral and acidic polysaccharides were separated using DEAE-Cellulose. Size exclusion chromatography analyses showed that the average molecular weight (MW) of the neutral M. pajang polysaccharides (F1) was ap...
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| Format: | Article |
| Language: | English English |
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Elsevier
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24516/ http://psasir.upm.edu.my/id/eprint/24516/1/Purification.pdf |
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| author | Al-Sheraji, Sadeq Hasan Ismail, Amin Abd. Manap, Mohd. Yazid Mustafa, Shuhaimi Mohd Yusof, Rokiah Hassan, Fouad Abdulrahman |
| author_facet | Al-Sheraji, Sadeq Hasan Ismail, Amin Abd. Manap, Mohd. Yazid Mustafa, Shuhaimi Mohd Yusof, Rokiah Hassan, Fouad Abdulrahman |
| author_sort | Al-Sheraji, Sadeq Hasan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Polysaccharides were isolated from the fibrous pulp of bambangan (Mangifera pajang Kort.). Neutral and acidic polysaccharides were separated using DEAE-Cellulose. Size exclusion chromatography analyses showed that the average molecular weight (MW) of the neutral M. pajang polysaccharides (F1) was approximately 7 kDa, and those of three acidic polysaccharides (F2, F3 and F4) were approximately 13, 24 and 9 kDa, respectively. The monosaccharide compositions of these polysaccharides were determined using high performance liquid chromatography. F1 contained erythrose, rhamnose, arabinose, mannose, fructose and glucose (5, 7, 21, 42, 4 and 21 mg/100 mg fraction respectively), F2 consisted of rhamnose, xylose and arabinose (33, 7 and 51 mg/100 mg fraction respectively), F3 consisted of fructose (14 mg/100 mg fraction) and glucose (72 mg/100 mg fraction), and F4 comprised arabinose, mannose, fructose and glucose (32, 36, 2 and 10 mg/100 mg fraction respectively). Results of Fourier transform infrared spectroscopy and the monosaccharide compositions suggested that the fibrous pulp of M. pajang fruit consisted of heteropolysaccharide and belonged to α and β–type of the pyran group. Additionally, crude polysaccharide and its fractions showed strong antioxidant activities. The acidic polysaccharides had the highest antioxidant activity and should be considered as a prospective antioxidant. |
| first_indexed | 2025-11-15T08:40:47Z |
| format | Article |
| id | upm-24516 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:40:47Z |
| publishDate | 2012 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-245162015-09-22T00:57:26Z http://psasir.upm.edu.my/id/eprint/24516/ Purification, characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of Mangifera pajang fruits. Al-Sheraji, Sadeq Hasan Ismail, Amin Abd. Manap, Mohd. Yazid Mustafa, Shuhaimi Mohd Yusof, Rokiah Hassan, Fouad Abdulrahman Polysaccharides were isolated from the fibrous pulp of bambangan (Mangifera pajang Kort.). Neutral and acidic polysaccharides were separated using DEAE-Cellulose. Size exclusion chromatography analyses showed that the average molecular weight (MW) of the neutral M. pajang polysaccharides (F1) was approximately 7 kDa, and those of three acidic polysaccharides (F2, F3 and F4) were approximately 13, 24 and 9 kDa, respectively. The monosaccharide compositions of these polysaccharides were determined using high performance liquid chromatography. F1 contained erythrose, rhamnose, arabinose, mannose, fructose and glucose (5, 7, 21, 42, 4 and 21 mg/100 mg fraction respectively), F2 consisted of rhamnose, xylose and arabinose (33, 7 and 51 mg/100 mg fraction respectively), F3 consisted of fructose (14 mg/100 mg fraction) and glucose (72 mg/100 mg fraction), and F4 comprised arabinose, mannose, fructose and glucose (32, 36, 2 and 10 mg/100 mg fraction respectively). Results of Fourier transform infrared spectroscopy and the monosaccharide compositions suggested that the fibrous pulp of M. pajang fruit consisted of heteropolysaccharide and belonged to α and β–type of the pyran group. Additionally, crude polysaccharide and its fractions showed strong antioxidant activities. The acidic polysaccharides had the highest antioxidant activity and should be considered as a prospective antioxidant. Elsevier 2012-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24516/1/Purification.pdf Al-Sheraji, Sadeq Hasan and Ismail, Amin and Abd. Manap, Mohd. Yazid and Mustafa, Shuhaimi and Mohd Yusof, Rokiah and Hassan, Fouad Abdulrahman (2012) Purification, characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of Mangifera pajang fruits. LWT - Food Science and Technology, 48 (2). pp. 291-296. ISSN 0023-6438 10.1016/j.lwt.2012.04.002 English |
| spellingShingle | Al-Sheraji, Sadeq Hasan Ismail, Amin Abd. Manap, Mohd. Yazid Mustafa, Shuhaimi Mohd Yusof, Rokiah Hassan, Fouad Abdulrahman Purification, characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of Mangifera pajang fruits. |
| title | Purification, characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of Mangifera pajang fruits. |
| title_full | Purification, characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of Mangifera pajang fruits. |
| title_fullStr | Purification, characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of Mangifera pajang fruits. |
| title_full_unstemmed | Purification, characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of Mangifera pajang fruits. |
| title_short | Purification, characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of Mangifera pajang fruits. |
| title_sort | purification, characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of mangifera pajang fruits. |
| url | http://psasir.upm.edu.my/id/eprint/24516/ http://psasir.upm.edu.my/id/eprint/24516/ http://psasir.upm.edu.my/id/eprint/24516/1/Purification.pdf |