Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method.
MSCs (mesenchymal stem cells) promise a great potential for regenerative medicine due to their unique properties of self-renewal, high plasticity, modulation of immune response and the flexibility for genetic modification. Therefore, the increasing demand for cellular therapy necessitates a larger-s...
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| Format: | Article |
| Language: | English English |
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Wiley-Blackwell
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24496/ http://psasir.upm.edu.my/id/eprint/24496/1/Generation%20of%20mesenchymal%20stem%20cell%20from%20human%20umbilical%20cord%20tissue%20using%20a%20combination%20enzymatic%20and%20mechanical%20disassociation%20method.pdf |
| _version_ | 1848845053171073024 |
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| author | Tong, Chih Kong Vellasamy, Shalini Tan, Boon Chong Abdullah, Maha Vidyadaran, Sharmili Seow, Heng Fong Ramasamy, Rajesh |
| author_facet | Tong, Chih Kong Vellasamy, Shalini Tan, Boon Chong Abdullah, Maha Vidyadaran, Sharmili Seow, Heng Fong Ramasamy, Rajesh |
| author_sort | Tong, Chih Kong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | MSCs (mesenchymal stem cells) promise a great potential for regenerative medicine due to their unique properties of self-renewal, high plasticity, modulation of immune response and the flexibility for genetic modification. Therefore, the increasing demand for cellular therapy necessitates a larger-scale production of MSC; however, the technical and ethical issues had put a halt on it. To date, studies have shown that MSC could be derived from human UC (umbilical cord), which is once considered as clinical waste. We have compared the two conventional methods which are classic enzymatic digestion and explant method with our newly tailored enzymatic-mechanical disassociation method to generate UC-MSC. The generated UC-MSCs from the methods above were characterized based on their immunophenotyping, early embryonic transcription factors expression and mesodermal differentiation ability. Our results show that enzymatic-mechanical disassociation method increase the initial nucleated cell yield greatly (approximately 160-fold) and maximized the successful rate of UC-MSC generation. Enzymatic-mechanical disassociation-derived UC-MSC exhibited fibroblastic morphology and surface markers expression of CD105, CD73, CD29, CD90 and MHC class I. Furthermore, these cells constitutively express early embryonic transcription factors (Nanog, Oct-4, Sox-2 and Rex-1), as confirmed by RT-PCR, indicating their multipotency and high self-renewal capacity. They are also capable of differentiating into osteoblasts and adipocytes when given an appropriate induction. The present study demonstrates a new and efficient approach in generating MSC from UC, hence serving as ideal alternative source of mesenchymal stem cell for clinical and research use. |
| first_indexed | 2025-11-15T08:40:42Z |
| format | Article |
| id | upm-24496 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:40:42Z |
| publishDate | 2011 |
| publisher | Wiley-Blackwell |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-244962016-01-12T04:11:23Z http://psasir.upm.edu.my/id/eprint/24496/ Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method. Tong, Chih Kong Vellasamy, Shalini Tan, Boon Chong Abdullah, Maha Vidyadaran, Sharmili Seow, Heng Fong Ramasamy, Rajesh MSCs (mesenchymal stem cells) promise a great potential for regenerative medicine due to their unique properties of self-renewal, high plasticity, modulation of immune response and the flexibility for genetic modification. Therefore, the increasing demand for cellular therapy necessitates a larger-scale production of MSC; however, the technical and ethical issues had put a halt on it. To date, studies have shown that MSC could be derived from human UC (umbilical cord), which is once considered as clinical waste. We have compared the two conventional methods which are classic enzymatic digestion and explant method with our newly tailored enzymatic-mechanical disassociation method to generate UC-MSC. The generated UC-MSCs from the methods above were characterized based on their immunophenotyping, early embryonic transcription factors expression and mesodermal differentiation ability. Our results show that enzymatic-mechanical disassociation method increase the initial nucleated cell yield greatly (approximately 160-fold) and maximized the successful rate of UC-MSC generation. Enzymatic-mechanical disassociation-derived UC-MSC exhibited fibroblastic morphology and surface markers expression of CD105, CD73, CD29, CD90 and MHC class I. Furthermore, these cells constitutively express early embryonic transcription factors (Nanog, Oct-4, Sox-2 and Rex-1), as confirmed by RT-PCR, indicating their multipotency and high self-renewal capacity. They are also capable of differentiating into osteoblasts and adipocytes when given an appropriate induction. The present study demonstrates a new and efficient approach in generating MSC from UC, hence serving as ideal alternative source of mesenchymal stem cell for clinical and research use. Wiley-Blackwell 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24496/1/Generation%20of%20mesenchymal%20stem%20cell%20from%20human%20umbilical%20cord%20tissue%20using%20a%20combination%20enzymatic%20and%20mechanical%20disassociation%20method.pdf Tong, Chih Kong and Vellasamy, Shalini and Tan, Boon Chong and Abdullah, Maha and Vidyadaran, Sharmili and Seow, Heng Fong and Ramasamy, Rajesh (2011) Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method. Cell Biology International, 35 (3). pp. 221-226. ISSN 1065-6995; ESSN:1095-8355 10.1042/CBI20100326 English |
| spellingShingle | Tong, Chih Kong Vellasamy, Shalini Tan, Boon Chong Abdullah, Maha Vidyadaran, Sharmili Seow, Heng Fong Ramasamy, Rajesh Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method. |
| title | Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method. |
| title_full | Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method. |
| title_fullStr | Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method. |
| title_full_unstemmed | Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method. |
| title_short | Generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method. |
| title_sort | generation of mesenchymal stem cell from human umbilical cord tissue using a combination enzymatic and mechanical disassociation method. |
| url | http://psasir.upm.edu.my/id/eprint/24496/ http://psasir.upm.edu.my/id/eprint/24496/ http://psasir.upm.edu.my/id/eprint/24496/1/Generation%20of%20mesenchymal%20stem%20cell%20from%20human%20umbilical%20cord%20tissue%20using%20a%20combination%20enzymatic%20and%20mechanical%20disassociation%20method.pdf |