Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines.
Nigella sativa has been used for centuries in Asia, Middle East and Africa to promote health and fight diseases. In this study, the anti-cancer effects of thymoquinone rich fraction (TQRF) extracted from N. sativa seeds using supercritical fluid extraction (SFE) system and commercially available thy...
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| Format: | Article |
| Language: | English English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24493/ http://psasir.upm.edu.my/id/eprint/24493/1/Thymoquinone%20rich%20fraction%20from%20Nigella%20sativa%20and%20thymoquinone%20are%20cytotoxic%20towards%20colon%20and%20leukemic%20carcinoma%20cell%20lines.pdf |
| _version_ | 1848845052336406528 |
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| author | Ismail, Norsharina Ismail, Maznah Al-Absi, Aied Al-Naqeeb, Ghanya |
| author_facet | Ismail, Norsharina Ismail, Maznah Al-Absi, Aied Al-Naqeeb, Ghanya |
| author_sort | Ismail, Norsharina |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Nigella sativa has been used for centuries in Asia, Middle East and Africa to promote health and fight diseases. In this study, the anti-cancer effects of thymoquinone rich fraction (TQRF) extracted from N. sativa seeds using supercritical fluid extraction (SFE) system and commercially available thymoquinone (TQ) on colon cancer (HT29), lymphoblastic leukemia (CEMSS) and promyelocytic leukemia (HL60) cells lines were investigated. The concentration that gave 50% inhibition of cell viability (IC50) of HT29, CEMSS and HL60 cells treated with TQRF were 400, 350 and 250 µg/ml, respectively. Meanwhile, the IC50 of TQ was 8, 5 and 3 µg/ml, respectively. Cell cycle analysis shows the increment of apoptosis in a time-dependent manner. However, both TQRF and TQ were not able to arrest the cell cycle phases of the cells. Apoptosis was the main mode of HT29, CEMSS and HL60 cells death induced by both TQRF and TQ. Our findings support the potential use of TQRF and TQ for the treatment of colon cancer and leukemia. |
| first_indexed | 2025-11-15T08:40:41Z |
| format | Article |
| id | upm-24493 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:40:41Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-244932015-09-22T07:44:33Z http://psasir.upm.edu.my/id/eprint/24493/ Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines. Ismail, Norsharina Ismail, Maznah Al-Absi, Aied Al-Naqeeb, Ghanya Nigella sativa has been used for centuries in Asia, Middle East and Africa to promote health and fight diseases. In this study, the anti-cancer effects of thymoquinone rich fraction (TQRF) extracted from N. sativa seeds using supercritical fluid extraction (SFE) system and commercially available thymoquinone (TQ) on colon cancer (HT29), lymphoblastic leukemia (CEMSS) and promyelocytic leukemia (HL60) cells lines were investigated. The concentration that gave 50% inhibition of cell viability (IC50) of HT29, CEMSS and HL60 cells treated with TQRF were 400, 350 and 250 µg/ml, respectively. Meanwhile, the IC50 of TQ was 8, 5 and 3 µg/ml, respectively. Cell cycle analysis shows the increment of apoptosis in a time-dependent manner. However, both TQRF and TQ were not able to arrest the cell cycle phases of the cells. Apoptosis was the main mode of HT29, CEMSS and HL60 cells death induced by both TQRF and TQ. Our findings support the potential use of TQRF and TQ for the treatment of colon cancer and leukemia. Academic Journals 2011-08-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24493/1/Thymoquinone%20rich%20fraction%20from%20Nigella%20sativa%20and%20thymoquinone%20are%20cytotoxic%20towards%20colon%20and%20leukemic%20carcinoma%20cell%20lines.pdf Ismail, Norsharina and Ismail, Maznah and Al-Absi, Aied and Al-Naqeeb, Ghanya (2011) Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines. Journal of Medicinal Plants Research, 5 (15). pp. 3359-3366. ISSN 1996-0875 http://www.academicjournals.org/journal/JMPR English |
| spellingShingle | Ismail, Norsharina Ismail, Maznah Al-Absi, Aied Al-Naqeeb, Ghanya Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines. |
| title | Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines. |
| title_full | Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines. |
| title_fullStr | Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines. |
| title_full_unstemmed | Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines. |
| title_short | Thymoquinone rich fraction from Nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines. |
| title_sort | thymoquinone rich fraction from nigella sativa and thymoquinone are cytotoxic towards colon and leukemic carcinoma cell lines. |
| url | http://psasir.upm.edu.my/id/eprint/24493/ http://psasir.upm.edu.my/id/eprint/24493/ http://psasir.upm.edu.my/id/eprint/24493/1/Thymoquinone%20rich%20fraction%20from%20Nigella%20sativa%20and%20thymoquinone%20are%20cytotoxic%20towards%20colon%20and%20leukemic%20carcinoma%20cell%20lines.pdf |