In vitro antiproliferative and antioxidant activities of the extracts of Muntingia calabura leaves.
The in vitro antiproliferative and antioxidant activities of the aqueous, chloroform and methanol extracts of Muntingia calabura leaves were determined in the present study. Assessed using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay, the aqueous and methanol extract...
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| Format: | Article |
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World Scientific Publishing
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24481/ http://psasir.upm.edu.my/id/eprint/24481/1/In%20vitro%20antiproliferative%20and%20antioxidant%20activities%20of%20the%20extracts%20of%20Muntingia%20calabura%20leaves.pdf |
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| author | Zakaria, Zainul Amiruddin Mohamed, A. M. Mohd. Jamil, N. S. Rofiee, M. S. Hussain, Mohd Khairi Sulaiman, Mohd Roslan Teh, Lay Kek Salleh, Mohd Zaki |
| author_facet | Zakaria, Zainul Amiruddin Mohamed, A. M. Mohd. Jamil, N. S. Rofiee, M. S. Hussain, Mohd Khairi Sulaiman, Mohd Roslan Teh, Lay Kek Salleh, Mohd Zaki |
| author_sort | Zakaria, Zainul Amiruddin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The in vitro antiproliferative and antioxidant activities of the aqueous, chloroform and methanol extracts of Muntingia calabura leaves were determined in the present study. Assessed using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay, the aqueous and methanol extracts of M. calabura inhibited the proliferation of MCF-7, HeLa, HT-29, HL-60 and K-562 cancer cells while the chloroform extract only inhibited the proliferation of MCF-7, HeLa, HL-60 and K-562 cancer cells. Interestingly, all extracts of M. calabura, which failed to inhibit the MDA-MB-231 cells proliferation, did not inhibit the proliferation of 3T3 (normal) cells, indicating its safety. All extracts (20, 100 and 500 μg/ml) were found to possess antioxidant activity when tested using the DPPH radical scavenging and superoxide scavenging assays with the methanol, followed by the aqueous and chloroform, extract exhibiting the highest antioxidant activity in both assays. The total phenolic content for the aqueous, methanol and chloroform extracts were 2970.4 ± 6.6, 1279.9 ± 6.1 and 2978.1 ± 4.3 mg/100 g gallic acid, respectively. In conclusion, the M. calabura leaves possess potential antiproliferative and antioxidant activities that could be attributed to its high content of phenolic compounds, and thus, needs to be further explored. |
| first_indexed | 2025-11-15T08:40:38Z |
| format | Article |
| id | upm-24481 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:40:38Z |
| publishDate | 2011 |
| publisher | World Scientific Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-244812015-11-18T03:48:49Z http://psasir.upm.edu.my/id/eprint/24481/ In vitro antiproliferative and antioxidant activities of the extracts of Muntingia calabura leaves. Zakaria, Zainul Amiruddin Mohamed, A. M. Mohd. Jamil, N. S. Rofiee, M. S. Hussain, Mohd Khairi Sulaiman, Mohd Roslan Teh, Lay Kek Salleh, Mohd Zaki The in vitro antiproliferative and antioxidant activities of the aqueous, chloroform and methanol extracts of Muntingia calabura leaves were determined in the present study. Assessed using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay, the aqueous and methanol extracts of M. calabura inhibited the proliferation of MCF-7, HeLa, HT-29, HL-60 and K-562 cancer cells while the chloroform extract only inhibited the proliferation of MCF-7, HeLa, HL-60 and K-562 cancer cells. Interestingly, all extracts of M. calabura, which failed to inhibit the MDA-MB-231 cells proliferation, did not inhibit the proliferation of 3T3 (normal) cells, indicating its safety. All extracts (20, 100 and 500 μg/ml) were found to possess antioxidant activity when tested using the DPPH radical scavenging and superoxide scavenging assays with the methanol, followed by the aqueous and chloroform, extract exhibiting the highest antioxidant activity in both assays. The total phenolic content for the aqueous, methanol and chloroform extracts were 2970.4 ± 6.6, 1279.9 ± 6.1 and 2978.1 ± 4.3 mg/100 g gallic acid, respectively. In conclusion, the M. calabura leaves possess potential antiproliferative and antioxidant activities that could be attributed to its high content of phenolic compounds, and thus, needs to be further explored. World Scientific Publishing 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24481/1/In%20vitro%20antiproliferative%20and%20antioxidant%20activities%20of%20the%20extracts%20of%20Muntingia%20calabura%20leaves.pdf Zakaria, Zainul Amiruddin and Mohamed, A. M. and Mohd. Jamil, N. S. and Rofiee, M. S. and Hussain, Mohd Khairi and Sulaiman, Mohd Roslan and Teh, Lay Kek and Salleh, Mohd Zaki (2011) In vitro antiproliferative and antioxidant activities of the extracts of Muntingia calabura leaves. The American Journal of Chinese Medicine, 39 (1). pp. 183-200. ISSN 0192-415X: ESSN: 1793-6853 http://www.worldscientific.com/worldscinet/ajcm 10.1142/S0192415X11008749 English |
| spellingShingle | Zakaria, Zainul Amiruddin Mohamed, A. M. Mohd. Jamil, N. S. Rofiee, M. S. Hussain, Mohd Khairi Sulaiman, Mohd Roslan Teh, Lay Kek Salleh, Mohd Zaki In vitro antiproliferative and antioxidant activities of the extracts of Muntingia calabura leaves. |
| title | In vitro antiproliferative and antioxidant activities of the extracts of Muntingia calabura leaves. |
| title_full | In vitro antiproliferative and antioxidant activities of the extracts of Muntingia calabura leaves. |
| title_fullStr | In vitro antiproliferative and antioxidant activities of the extracts of Muntingia calabura leaves. |
| title_full_unstemmed | In vitro antiproliferative and antioxidant activities of the extracts of Muntingia calabura leaves. |
| title_short | In vitro antiproliferative and antioxidant activities of the extracts of Muntingia calabura leaves. |
| title_sort | in vitro antiproliferative and antioxidant activities of the extracts of muntingia calabura leaves. |
| url | http://psasir.upm.edu.my/id/eprint/24481/ http://psasir.upm.edu.my/id/eprint/24481/ http://psasir.upm.edu.my/id/eprint/24481/ http://psasir.upm.edu.my/id/eprint/24481/1/In%20vitro%20antiproliferative%20and%20antioxidant%20activities%20of%20the%20extracts%20of%20Muntingia%20calabura%20leaves.pdf |