Purification and properties of a phytate-degrading enzyme produced by Enterobacter sakazakii ASUIA279.

QR Code

Purification and properties of a phytate-degrading enzyme produced by Enterobacter sakazakii ASUIA279.

An extracellular phytate-degrading enzyme produced by Enterobacter sakazakii ASUIA279 was purified to homogeneity using FPLC anion exchange chromatography and gel filtration. The enzyme was purified about 66-fold with a recovery of 27%. Its molecular mass was estimated to be 43 kDa by SDS-PAGE. The...

Full description

Bibliographic Details
Main Authors:	Farouk, Abd-ElAziem, Greiner, Ralf, Meor Hussin, Anis Shobirin
Format:	Article
Language:	English English
Published:	Federal University of Tocantins 2012
Online Access:	http://psasir.upm.edu.my/id/eprint/24200/ http://psasir.upm.edu.my/id/eprint/24200/1/Purification%20and%20properties%20of%20a%20phytate.pdf

Similar Items

Cytotoxicity and mutagenicity evaluation of crude phytase from Enterobacter sakazakii ASUIA 279 for feed application
by: Yusof, Saniza Sakinah, et al.
Published: (2012)

Optimization of cultivation conditions for the production of bacterial phytase from enterobacter sakazakii ASUIA279 newly isolated from Malaysian maize root
by: Meor Hussin, Anis Shobirin, et al.
Published: (2009)

Potential phytate-degrading enzyme producing bacteria isolated from Malaysian maize plantation
by: Meor Hussin, Anis Shobirin, et al.
Published: (2009)

Production of Phytate-Degrading enzyme from Malaysian soil bacteria using rice bran containing media.
by: Meor Hussin, Anis Shobirin, et al.
Published: (2010)

Molecular cloning of phytase gene from ASUIA279 and its expression in Pichia Pastoris system.
by: Shobirin, Anis, et al.
Published: (2011)

Efficacy of a locally produced microbial phytase from Enterobacter sakazakii ASUIA273 on body weight and hemato-biochemical constituents in broiler chickens
by: Islam, R., et al.
Published: (2011)

Preliminary investigation of myo-inositol phosphates produced by ASUIA279 phytase on MCF-7 cancer cells.
by: Mohd Yusoff , N., et al.
Published: (2011)

Preliminary investigation of Myo-Inositol Phosphates produced by Asuia279phytase on Mcf-7 cancer cells.
by: Mohd Yusoff, Noraini, et al.
Published: (2011)

Purification and characterization of a bacterial phytase whose properties make it exceptionally useful as a feed supplement
by: Greiner, Ralf, et al.
Published: (2007)

Optimization of the conditions for rice bran phytate degradation by their own phytases
by: Farouk, Abd-El Aziem, et al.
Published: (2018)

Autolysis of rice bran phytate in long-term study on batch fermentor
by: Farouk, Abd-ElAziem, et al.
Published: (2017)

Molecular cloning of phytase gene from ASUIA279 and its expression in Pichia Pastoris system
by: Maifiah, M.H. Mahamad, et al.
Published: (2011)

Preliminary investigation of MYO-INOSITOL phosphates produced by ASUIA279PHYTASE on MCF-7 cancer cells
by: Yusoff, Narazah Mohd, et al.
Published: (2011)

Effects of cell immobilization to the phytate-degrading enzyme activity
by: Mohamad Puad, Noor Illi, et al.
Published: (2011)

Enterobacteriaceae, cronobacter(enterobacter) sakazakii and microbial population in infant formula products in the Malaysian market
by: Norrakiah Abdullah Sani,, et al.
Published: (2011)

Molecular cloning and production of recombinant phytase from bacillus subtilis ASUIA243 in Pichia Pastoris.
by: Mohd Dali , Nor Soleha, et al.
Published: (2011)

Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
by: Mahamad Maifiah, Mohd Hafidz, et al.
Published: (2011)

Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
by: Mahamad Maifiah, Mohd Hafidz, et al.
Published: (2011)

Statistical optimization of the induction of the induction of phytase production by Arabinose in a recombinant E. coli using response surface methodology
by: Farouk, Abd-ElAziem, et al.
Published: (2009)

Degradation of phytate in rice bran by local bacteria phytase
by: Mohd Yusoff, Noraini, et al.
Published: (2010)

Molecular cloning and production of recombinant phytase from Bacillus Subtilis ASUIA243 in Pichia Pastoris
by: Mohd Dali, Nor Soleha, et al.
Published: (2011)

Valorisation of virgin coconut oil application in mayonnaise production as functional Ingredient
by: Muhialdin, Belal J., et al.
Published: (2019)

Effectiveness of quaternary ammonium in reducing microbial load on eggs
by: Hao, Yuan Chan, et al.
Published: (2021)

Reducing microbial contamination risk and improving physical properties of plant-based mayonnaise produced using chickpea aquafaba
by: Muhialdin, Belal J., et al.
Published: (2021)

Isolation and Characterization of a Molybdenum Reducing Enzyme in Enterobacter cloacae Strain 48
by: Abd. Shukor, Mohd. Yunus, et al.
Published: (2003)

Chemical compositions, antioxidant and antimicrobial activities of Tubu (Pycnarrhena Longifolia) leaves used as ingredient in traditional functional foods
by: Mohammed, Nameer Khairullah, et al.
Published: (2020)

Incidence of Cronobacter sakazakii in Powdered Infant Formula Milk Available in Malaysia
by: Norrakiah Abdullah Sani,, et al.
Published: (2014)

Optimisation of phytate degradation in whole grain rice during germination processing using response surface methodology
by: Huei-Hong, Lee, et al.
Published: (2023)

Purification And Characterization Of Acrylamide-Degrading Enzyme From Burkholderia Sp. Dr.Y27
by: Gusmanizar, Neni
Published: (2006)

Molybdenum reductase in Enterobacter cloacae
by: Ariff, Arbakariya, et al.
Published: (1997)

Enhanced carbofuran degradation using immobilized and free cells of Enterobacter sp. isolated from soil
by: Mustapha, Mohammed Umar, et al.
Published: (2020)

Isolation and Purification of Raw Starch Degrading Enzyme from Endophytic Fungi and its Application for Glucose Production
by: Marlida, Yetti
Published: (2001)

Isolation and characterization of a molybdenum-reducing and phenolic- and catechol-degrading Enterobacter sp. strain Saw-2
by: Sabullah, Mohd Khalizan, et al.
Published: (2017)

Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia
by: Alaraidh, Ibrahim, et al.
Published: (2011)

Comparative Genome Analysis of Enterobacter cloacae
by: Liu, Wing Yee, et al.
Published: (2013)

CITREX UMP showcases 279 students and researchers innovative products
by: Mimi Rabita, Abdul Wahit
Published: (2023)

Production and Purification of Mannan-Degrading Enzymes from Palm Kernel Cake Fermented by Aspergillus Niger and Sclerotium Rolfsii
by: Ab. Razak, Nor asma
Published: (2006)

Extraction of products from enzymatic hydrolysis of phytate in rice bran
by: Mohd. Yusof, Noraini, et al.
Published: (2010)

Effect of Phytate Phosphorus on the Utilisation of Rice Bran in Broiler Chickens)
by: Ukil, Md. Asaduzzaman
Published: (1999)

Laccase enzymes: purification, structure to catalysis and tailoring
by: Moin, Syed Faraz, et al.
Published: (2014)