Optimization of the conditions for extraction of serine protease from kesinai plant (Streblus asper) leaves using response surface methodology.
Response surface methodology (RSM) using a central composite design (CCD) was employed to optimize the conditions for extraction of serine protease from kesinai (Streblus asper) leaves. The effect of independent variables, namely temperature (42.5,47.5, X₁), mixing time (2-6 min, X₂), buffer content...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
MDPI
2011
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/24194/ http://psasir.upm.edu.my/id/eprint/24194/1/Optimization%20of%20the%20conditions%20for%20extraction%20of%20serine%20protease%20from%20kesinai%20plant.pdf |
| _version_ | 1848844967868366848 |
|---|---|
| author | Mehrnoush, Amid Mustafa, Shuhaimi Sarker, Zaidul Islam Abd. Manap, Mohd. Yazid |
| author_facet | Mehrnoush, Amid Mustafa, Shuhaimi Sarker, Zaidul Islam Abd. Manap, Mohd. Yazid |
| author_sort | Mehrnoush, Amid |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Response surface methodology (RSM) using a central composite design (CCD) was employed to optimize the conditions for extraction of serine protease from kesinai (Streblus asper) leaves. The effect of independent variables, namely temperature (42.5,47.5, X₁), mixing time (2-6 min, X₂), buffer content (0-80 mL, X₃) and buffer pH (4.5-10.5, X₄) on specific activity, storage stability, temperature and oxidizing agent stability of serine protease from kesinai leaves was investigated. The study demonstrated that use of the optimum temperature, mixing time, buffer content and buffer pH conditions protected serine protease during extraction, as demonstrated by low activity loss. It was found that the interaction effect of mixing time and buffer content improved the serine protease stability, and the buffer pH had the most significant effect on the specific activity of the enzyme. The most desirable conditions of 2.5 °C temperature, 4 min mixing time, 40 mL buffer at pH 7.5 was established for serine protease extraction from kesinai leaves. |
| first_indexed | 2025-11-15T08:39:20Z |
| format | Article |
| id | upm-24194 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:39:20Z |
| publishDate | 2011 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-241942015-10-20T01:13:49Z http://psasir.upm.edu.my/id/eprint/24194/ Optimization of the conditions for extraction of serine protease from kesinai plant (Streblus asper) leaves using response surface methodology. Mehrnoush, Amid Mustafa, Shuhaimi Sarker, Zaidul Islam Abd. Manap, Mohd. Yazid Response surface methodology (RSM) using a central composite design (CCD) was employed to optimize the conditions for extraction of serine protease from kesinai (Streblus asper) leaves. The effect of independent variables, namely temperature (42.5,47.5, X₁), mixing time (2-6 min, X₂), buffer content (0-80 mL, X₃) and buffer pH (4.5-10.5, X₄) on specific activity, storage stability, temperature and oxidizing agent stability of serine protease from kesinai leaves was investigated. The study demonstrated that use of the optimum temperature, mixing time, buffer content and buffer pH conditions protected serine protease during extraction, as demonstrated by low activity loss. It was found that the interaction effect of mixing time and buffer content improved the serine protease stability, and the buffer pH had the most significant effect on the specific activity of the enzyme. The most desirable conditions of 2.5 °C temperature, 4 min mixing time, 40 mL buffer at pH 7.5 was established for serine protease extraction from kesinai leaves. MDPI 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24194/1/Optimization%20of%20the%20conditions%20for%20extraction%20of%20serine%20protease%20from%20kesinai%20plant.pdf Mehrnoush, Amid and Mustafa, Shuhaimi and Sarker, Zaidul Islam and Abd. Manap, Mohd. Yazid (2011) Optimization of the conditions for extraction of serine protease from kesinai plant (Streblus asper) leaves using response surface methodology. Molecules, 16 (11). pp. 9245-9260. ISSN 1420-3049; ESSN:1420-3049 http://www.mdpi.com/ 10.3390/molecules16119245 English |
| spellingShingle | Mehrnoush, Amid Mustafa, Shuhaimi Sarker, Zaidul Islam Abd. Manap, Mohd. Yazid Optimization of the conditions for extraction of serine protease from kesinai plant (Streblus asper) leaves using response surface methodology. |
| title | Optimization of the conditions for extraction of serine protease from kesinai plant (Streblus asper) leaves using response surface methodology. |
| title_full | Optimization of the conditions for extraction of serine protease from kesinai plant (Streblus asper) leaves using response surface methodology. |
| title_fullStr | Optimization of the conditions for extraction of serine protease from kesinai plant (Streblus asper) leaves using response surface methodology. |
| title_full_unstemmed | Optimization of the conditions for extraction of serine protease from kesinai plant (Streblus asper) leaves using response surface methodology. |
| title_short | Optimization of the conditions for extraction of serine protease from kesinai plant (Streblus asper) leaves using response surface methodology. |
| title_sort | optimization of the conditions for extraction of serine protease from kesinai plant (streblus asper) leaves using response surface methodology. |
| url | http://psasir.upm.edu.my/id/eprint/24194/ http://psasir.upm.edu.my/id/eprint/24194/ http://psasir.upm.edu.my/id/eprint/24194/ http://psasir.upm.edu.my/id/eprint/24194/1/Optimization%20of%20the%20conditions%20for%20extraction%20of%20serine%20protease%20from%20kesinai%20plant.pdf |