A quick DNA extraction protocol: without liquid nitrogen in ambient temperature
Marker assisted selection is an effective technique for quality traits selection in breeding program which are impossible by visual observation. Marker assisted selection in early generation requires rapid DNA extraction protocol for large number of samples in a low cost approach. A rapid and inexpe...
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| Format: | Article |
| Language: | English |
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Academic Journals
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24185/ http://psasir.upm.edu.my/id/eprint/24185/1/24185.pdf |
| _version_ | 1848844965405261824 |
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| author | Ferdous, Jannatul Musa, Mohamed Hanafi Yusop, Mohd Rafii Syed Muhammad, Sharifah Kharidah |
| author_facet | Ferdous, Jannatul Musa, Mohamed Hanafi Yusop, Mohd Rafii Syed Muhammad, Sharifah Kharidah |
| author_sort | Ferdous, Jannatul |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Marker assisted selection is an effective technique for quality traits selection in breeding program which are impossible by visual observation. Marker assisted selection in early generation requires rapid DNA extraction protocol for large number of samples in a low cost approach. A rapid and inexpensive DNA extraction protocol has been described for different tissues of color rice and other plant species which contain pigment and polyphenolic compound. This method has been modified from well known cetyltrimethylammonium bromide (CTAB) method where CTAB is used for DNA extraction. This protocol is simple and fast compared to other methods and no liquid nitrogen is required. Only inexpensive chemicals and ordinary laboratory equipments are enough for DNA extraction. The quantity of total genomic DNA from different tissues was almost similar which was extracted from 10 mg samples. The extracted DNA is stable and applicable to marker assisted selection, DNA fingerprinting, quantitative traits loci analysis, screening of transformants and enzymatic digestion. |
| first_indexed | 2025-11-15T08:39:18Z |
| format | Article |
| id | upm-24185 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:39:18Z |
| publishDate | 2012 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-241852017-11-09T09:25:26Z http://psasir.upm.edu.my/id/eprint/24185/ A quick DNA extraction protocol: without liquid nitrogen in ambient temperature Ferdous, Jannatul Musa, Mohamed Hanafi Yusop, Mohd Rafii Syed Muhammad, Sharifah Kharidah Marker assisted selection is an effective technique for quality traits selection in breeding program which are impossible by visual observation. Marker assisted selection in early generation requires rapid DNA extraction protocol for large number of samples in a low cost approach. A rapid and inexpensive DNA extraction protocol has been described for different tissues of color rice and other plant species which contain pigment and polyphenolic compound. This method has been modified from well known cetyltrimethylammonium bromide (CTAB) method where CTAB is used for DNA extraction. This protocol is simple and fast compared to other methods and no liquid nitrogen is required. Only inexpensive chemicals and ordinary laboratory equipments are enough for DNA extraction. The quantity of total genomic DNA from different tissues was almost similar which was extracted from 10 mg samples. The extracted DNA is stable and applicable to marker assisted selection, DNA fingerprinting, quantitative traits loci analysis, screening of transformants and enzymatic digestion. Academic Journals 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24185/1/24185.pdf Ferdous, Jannatul and Musa, Mohamed Hanafi and Yusop, Mohd Rafii and Syed Muhammad, Sharifah Kharidah (2012) A quick DNA extraction protocol: without liquid nitrogen in ambient temperature. African Journal of Biotechnology, 11 (27). art. no. 197554430636. pp. 6956-6964. ISSN 1684–5315 http://academicjournals.org/journal/AJB/article-abstract/197554430636 10.5897/AJB11.3283 |
| spellingShingle | Ferdous, Jannatul Musa, Mohamed Hanafi Yusop, Mohd Rafii Syed Muhammad, Sharifah Kharidah A quick DNA extraction protocol: without liquid nitrogen in ambient temperature |
| title | A quick DNA extraction protocol: without liquid nitrogen in ambient temperature |
| title_full | A quick DNA extraction protocol: without liquid nitrogen in ambient temperature |
| title_fullStr | A quick DNA extraction protocol: without liquid nitrogen in ambient temperature |
| title_full_unstemmed | A quick DNA extraction protocol: without liquid nitrogen in ambient temperature |
| title_short | A quick DNA extraction protocol: without liquid nitrogen in ambient temperature |
| title_sort | quick dna extraction protocol: without liquid nitrogen in ambient temperature |
| url | http://psasir.upm.edu.my/id/eprint/24185/ http://psasir.upm.edu.my/id/eprint/24185/ http://psasir.upm.edu.my/id/eprint/24185/ http://psasir.upm.edu.my/id/eprint/24185/1/24185.pdf |