Phenolics in Citrus hystrix leaves obtained using supercritical carbon dioxide extraction
The extraction of phenolics from Citrus hystrix leaf was carried out using supercritical fluid extraction and was optimized using response surface methodology (RSM). The effects of CO2 flow rate, extraction pressure and extraction temperature on yield, total phenolic content and diphenyl-picrylhydra...
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| Format: | Article |
| Language: | English English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24145/ http://psasir.upm.edu.my/id/eprint/24145/1/24145.pdf |
| _version_ | 1848844954321813504 |
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| author | Bakar, Jamilah M., Abdulkadir Gadi Mohamed, Suhaila Sarker, Md. Zaidul Islam |
| author_facet | Bakar, Jamilah M., Abdulkadir Gadi Mohamed, Suhaila Sarker, Md. Zaidul Islam |
| author_sort | Bakar, Jamilah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The extraction of phenolics from Citrus hystrix leaf was carried out using supercritical fluid extraction and was optimized using response surface methodology (RSM). The effects of CO2 flow rate, extraction pressure and extraction temperature on yield, total phenolic content and diphenyl-picrylhydrazyl-IC50 were evaluated and compared with ethanol extraction. The extraction pressure was the most significant factor affecting the yield, TPC and DPPH-IC50 of the extracts, followed by CO2 flow rate and the extraction temperature. The optimum conditions of pressure, CO2 flow rate and temperature were at 267 bars, 18 g/min and 50oC, respectively. The yield, TPC and DPPH-IC50 obtained were 5.06%, 116.53 mg GAE/g extract and IC50 of 0.063 mg/ml, respectively. These values were not significantly different (p<0.05) to their predicted values. Better inhibition and TPC were obtained using SFE method whereas higher yield and phenolic acids were obtained in the ethanol extracts. |
| first_indexed | 2025-11-15T08:39:07Z |
| format | Article |
| id | upm-24145 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:39:07Z |
| publishDate | 2011 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-241452015-06-01T08:10:05Z http://psasir.upm.edu.my/id/eprint/24145/ Phenolics in Citrus hystrix leaves obtained using supercritical carbon dioxide extraction Bakar, Jamilah M., Abdulkadir Gadi Mohamed, Suhaila Sarker, Md. Zaidul Islam The extraction of phenolics from Citrus hystrix leaf was carried out using supercritical fluid extraction and was optimized using response surface methodology (RSM). The effects of CO2 flow rate, extraction pressure and extraction temperature on yield, total phenolic content and diphenyl-picrylhydrazyl-IC50 were evaluated and compared with ethanol extraction. The extraction pressure was the most significant factor affecting the yield, TPC and DPPH-IC50 of the extracts, followed by CO2 flow rate and the extraction temperature. The optimum conditions of pressure, CO2 flow rate and temperature were at 267 bars, 18 g/min and 50oC, respectively. The yield, TPC and DPPH-IC50 obtained were 5.06%, 116.53 mg GAE/g extract and IC50 of 0.063 mg/ml, respectively. These values were not significantly different (p<0.05) to their predicted values. Better inhibition and TPC were obtained using SFE method whereas higher yield and phenolic acids were obtained in the ethanol extracts. Faculty of Food Science and Technology, Universiti Putra Malaysia 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24145/1/24145.pdf Bakar, Jamilah and M., Abdulkadir Gadi and Mohamed, Suhaila and Sarker, Md. Zaidul Islam (2011) Phenolics in Citrus hystrix leaves obtained using supercritical carbon dioxide extraction. International Food Research Journal, 18 (3). pp. 941-948. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-18-2011.html English |
| spellingShingle | Bakar, Jamilah M., Abdulkadir Gadi Mohamed, Suhaila Sarker, Md. Zaidul Islam Phenolics in Citrus hystrix leaves obtained using supercritical carbon dioxide extraction |
| title | Phenolics in Citrus hystrix leaves obtained using supercritical carbon dioxide extraction |
| title_full | Phenolics in Citrus hystrix leaves obtained using supercritical carbon dioxide extraction |
| title_fullStr | Phenolics in Citrus hystrix leaves obtained using supercritical carbon dioxide extraction |
| title_full_unstemmed | Phenolics in Citrus hystrix leaves obtained using supercritical carbon dioxide extraction |
| title_short | Phenolics in Citrus hystrix leaves obtained using supercritical carbon dioxide extraction |
| title_sort | phenolics in citrus hystrix leaves obtained using supercritical carbon dioxide extraction |
| url | http://psasir.upm.edu.my/id/eprint/24145/ http://psasir.upm.edu.my/id/eprint/24145/ http://psasir.upm.edu.my/id/eprint/24145/1/24145.pdf |