Analysis of thermal inactivation kinetics of membrane-bound polyphenol oxidases and peroxidases from Metroxylon sagu
Thermal inactivation kinetics for the purified membrane-bound polyphenol oxidases (mPPOs) and peroxidases (mPODs) isolated from Metroxylon sagu were analyzed. Each isoenzyme was treated at different time-temperature combinations in the range of 0-70min and 20-70C. Thermal inactivation rates constant...
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| Format: | Article |
| Language: | English |
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Wiley Periodicals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24141/ http://psasir.upm.edu.my/id/eprint/24141/1/Analysis%20of%20thermal%20inactivation%20kinetics%20of%20membrane.pdf |
| _version_ | 1848844953299451904 |
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| author | Onsa, Galila Hassan Abdul Hamid, Azizah Selamat, Jinap Bakar, Jamilah Abd Manap, Mohd Yazid Saari, Nazamid |
| author_facet | Onsa, Galila Hassan Abdul Hamid, Azizah Selamat, Jinap Bakar, Jamilah Abd Manap, Mohd Yazid Saari, Nazamid |
| author_sort | Onsa, Galila Hassan |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Thermal inactivation kinetics for the purified membrane-bound polyphenol oxidases (mPPOs) and peroxidases (mPODs) isolated from Metroxylon sagu were analyzed. Each isoenzyme was treated at different time-temperature combinations in the range of 0-70min and 20-70C. Thermal inactivation rates constant (k) at 70C for mPOD-I (72.9×10 -3/min) and mPOD-II (97.9×10 -3/min) were lower than that of mPPO-I (379.7×10 -3/min) and mPPO-II (138.1×10 -3/min). The activation energy for inactivation of mPPO-I (32.94kcal/mol) and mPPO-II (40.34kcal/mol) was lower compared with mPOD-I (45.77kcal/mol) and mPOD-II (40.62kcal/mol). The enthalpy values for mPOD-I (45.08kcal/mol) and mPOD-II (39.94kcal/mol) were higher than those of mPPOs (mPPO-I, 32.26kcal/mol; mPPO-II, 39.66kcal/mol). This result implies that both mPOD-I and mPOD-II are more thermostable. |
| first_indexed | 2025-11-15T08:39:06Z |
| format | Article |
| id | upm-24141 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:39:06Z |
| publishDate | 2011 |
| publisher | Wiley Periodicals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-241412016-12-23T08:28:20Z http://psasir.upm.edu.my/id/eprint/24141/ Analysis of thermal inactivation kinetics of membrane-bound polyphenol oxidases and peroxidases from Metroxylon sagu Onsa, Galila Hassan Abdul Hamid, Azizah Selamat, Jinap Bakar, Jamilah Abd Manap, Mohd Yazid Saari, Nazamid Thermal inactivation kinetics for the purified membrane-bound polyphenol oxidases (mPPOs) and peroxidases (mPODs) isolated from Metroxylon sagu were analyzed. Each isoenzyme was treated at different time-temperature combinations in the range of 0-70min and 20-70C. Thermal inactivation rates constant (k) at 70C for mPOD-I (72.9×10 -3/min) and mPOD-II (97.9×10 -3/min) were lower than that of mPPO-I (379.7×10 -3/min) and mPPO-II (138.1×10 -3/min). The activation energy for inactivation of mPPO-I (32.94kcal/mol) and mPPO-II (40.34kcal/mol) was lower compared with mPOD-I (45.77kcal/mol) and mPOD-II (40.62kcal/mol). The enthalpy values for mPOD-I (45.08kcal/mol) and mPOD-II (39.94kcal/mol) were higher than those of mPPOs (mPPO-I, 32.26kcal/mol; mPPO-II, 39.66kcal/mol). This result implies that both mPOD-I and mPOD-II are more thermostable. Wiley Periodicals 2011-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24141/1/Analysis%20of%20thermal%20inactivation%20kinetics%20of%20membrane.pdf Onsa, Galila Hassan and Abdul Hamid, Azizah and Selamat, Jinap and Bakar, Jamilah and Abd Manap, Mohd Yazid and Saari, Nazamid (2011) Analysis of thermal inactivation kinetics of membrane-bound polyphenol oxidases and peroxidases from Metroxylon sagu. Journal of Food Biochemistry, 35 (3). pp. 819-832. ISSN 0145-8884; ESSN: 1745-4514 http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1745-4514.2010.00419.x/abstract 10.1111/j.1745-4514.2010.00419.x |
| spellingShingle | Onsa, Galila Hassan Abdul Hamid, Azizah Selamat, Jinap Bakar, Jamilah Abd Manap, Mohd Yazid Saari, Nazamid Analysis of thermal inactivation kinetics of membrane-bound polyphenol oxidases and peroxidases from Metroxylon sagu |
| title | Analysis of thermal inactivation kinetics of membrane-bound polyphenol oxidases and peroxidases from Metroxylon sagu |
| title_full | Analysis of thermal inactivation kinetics of membrane-bound polyphenol oxidases and peroxidases from Metroxylon sagu |
| title_fullStr | Analysis of thermal inactivation kinetics of membrane-bound polyphenol oxidases and peroxidases from Metroxylon sagu |
| title_full_unstemmed | Analysis of thermal inactivation kinetics of membrane-bound polyphenol oxidases and peroxidases from Metroxylon sagu |
| title_short | Analysis of thermal inactivation kinetics of membrane-bound polyphenol oxidases and peroxidases from Metroxylon sagu |
| title_sort | analysis of thermal inactivation kinetics of membrane-bound polyphenol oxidases and peroxidases from metroxylon sagu |
| url | http://psasir.upm.edu.my/id/eprint/24141/ http://psasir.upm.edu.my/id/eprint/24141/ http://psasir.upm.edu.my/id/eprint/24141/ http://psasir.upm.edu.my/id/eprint/24141/1/Analysis%20of%20thermal%20inactivation%20kinetics%20of%20membrane.pdf |