Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system.
A new method for the simultaneous quantification of 12 mycotoxins was developed and optimized using reverse phase high performance liquid chromatography (RP-HPLC) with a photodiode array (PDA) and fluorescence detector (FLD), a photochemical reactor for enhanced detection (PHRED) and post-column der...
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| Format: | Article |
| Language: | English English |
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Taylor & Francis
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24134/ http://psasir.upm.edu.my/id/eprint/24134/1/Simultaneous%20detection%20of%2012%20mycotoxins%20in%20cereals%20using%20RP.pdf |
| _version_ | 1848844951528407040 |
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| author | Soleimany, Farhang Selamat , Jinap Rahmani, Anosheh Khatib , Alfi |
| author_facet | Soleimany, Farhang Selamat , Jinap Rahmani, Anosheh Khatib , Alfi |
| author_sort | Soleimany, Farhang |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A new method for the simultaneous quantification of 12 mycotoxins was developed and optimized using reverse phase high performance liquid chromatography (RP-HPLC) with a photodiode array (PDA) and fluorescence detector (FLD), a photochemical reactor for enhanced detection (PHRED) and post-column derivatization. The mycotoxins included aflatoxins (AFB1, AFB2, AFG1, and AFG2), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), fumonisins (FB1, FB2, and FB3), T-2 and HT-2 toxins. A double sample extraction with a phosphate-buffered saline solution (PBS) and methanol was used for co-extraction of mycotoxins, and a multifunctional immunoaffinity column was used for cleanup. Optimum conditions for separation of the mycotoxins were obtained to separate 12 mycotoxins in FLD and PDA chromatograms with a high resolution. The method gave recoveries in the range 72-111% when applied to spiked corn samples. The limits of detection (LOD) were 0.025 ng/g for AFB1 and AFG1, 0.012 ng/g for AFB2 and AFG2, 0.2 ng/g for OTA, 1.5 ng/g for ZEA, 6.2 ng/g for FB1, FB3 and HT-2 toxin, 9.4 ng/g for FB2 and T-2 toxin, and 18.7 ng/g for DON. In addition, the limits of quantification (LOQ) ranged from 0.04 ng/g for AFB2 and AFG2 to 62 ng/g for DON. The method was successfully applied to the determination of these mycotoxins in 45 cereal samples obtained from the Malaysian market. The results indicated that the method can be applied for the multi-mycotoxin determination of cereals. |
| first_indexed | 2025-11-15T08:39:05Z |
| format | Article |
| id | upm-24134 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:39:05Z |
| publishDate | 2011 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-241342016-02-18T04:32:41Z http://psasir.upm.edu.my/id/eprint/24134/ Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system. Soleimany, Farhang Selamat , Jinap Rahmani, Anosheh Khatib , Alfi A new method for the simultaneous quantification of 12 mycotoxins was developed and optimized using reverse phase high performance liquid chromatography (RP-HPLC) with a photodiode array (PDA) and fluorescence detector (FLD), a photochemical reactor for enhanced detection (PHRED) and post-column derivatization. The mycotoxins included aflatoxins (AFB1, AFB2, AFG1, and AFG2), ochratoxin A (OTA), zearalenone (ZEA), deoxynivalenol (DON), fumonisins (FB1, FB2, and FB3), T-2 and HT-2 toxins. A double sample extraction with a phosphate-buffered saline solution (PBS) and methanol was used for co-extraction of mycotoxins, and a multifunctional immunoaffinity column was used for cleanup. Optimum conditions for separation of the mycotoxins were obtained to separate 12 mycotoxins in FLD and PDA chromatograms with a high resolution. The method gave recoveries in the range 72-111% when applied to spiked corn samples. The limits of detection (LOD) were 0.025 ng/g for AFB1 and AFG1, 0.012 ng/g for AFB2 and AFG2, 0.2 ng/g for OTA, 1.5 ng/g for ZEA, 6.2 ng/g for FB1, FB3 and HT-2 toxin, 9.4 ng/g for FB2 and T-2 toxin, and 18.7 ng/g for DON. In addition, the limits of quantification (LOQ) ranged from 0.04 ng/g for AFB2 and AFG2 to 62 ng/g for DON. The method was successfully applied to the determination of these mycotoxins in 45 cereal samples obtained from the Malaysian market. The results indicated that the method can be applied for the multi-mycotoxin determination of cereals. Taylor & Francis 2011-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24134/1/Simultaneous%20detection%20of%2012%20mycotoxins%20in%20cereals%20using%20RP.pdf Soleimany, Farhang and Selamat , Jinap and Rahmani, Anosheh and Khatib , Alfi (2011) Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system. Food Additives & Contaminants: Part A, 28 (4). pp. 494-501. ISSN 1944-0049 http://www.tandfonline.com/ 10.1080/19440049.2010.551547 English |
| spellingShingle | Soleimany, Farhang Selamat , Jinap Rahmani, Anosheh Khatib , Alfi Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system. |
| title | Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system. |
| title_full | Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system. |
| title_fullStr | Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system. |
| title_full_unstemmed | Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system. |
| title_short | Simultaneous detection of 12 mycotoxins in cereals using RP-HPLC-PDA-FLD with PHRED and a post-column derivatization system. |
| title_sort | simultaneous detection of 12 mycotoxins in cereals using rp-hplc-pda-fld with phred and a post-column derivatization system. |
| url | http://psasir.upm.edu.my/id/eprint/24134/ http://psasir.upm.edu.my/id/eprint/24134/ http://psasir.upm.edu.my/id/eprint/24134/ http://psasir.upm.edu.my/id/eprint/24134/1/Simultaneous%20detection%20of%2012%20mycotoxins%20in%20cereals%20using%20RP.pdf |