Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity.
The in vivo immunomodulatory effect of ethanolic extracts from leaves of Rhaphidophora korthalsii was determined via immune cell proliferation, T/NK cell phenotyping, and splenocyte cytotoxicity of BALB/c mice after 5 consecutive days of i.p. administration at various concentrations. Splenocyte prol...
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| Format: | Article |
| Language: | English English |
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Hindawi Publishing Corporation
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24114/ http://psasir.upm.edu.my/id/eprint/24114/1/Immunomodulatory%20effect%20of%20Rhaphidophora%20korthalsii%20on%20natural%20killer%20cell%20cytotoxicity.pdf |
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| author | Yeap, Swee Keong Omar, Abdul Rahman Ali, Abdul Manaf Ho, Wan Yong Beh, Boon Kee Mohamed Alitheen, Noorjahan Banu |
| author_facet | Yeap, Swee Keong Omar, Abdul Rahman Ali, Abdul Manaf Ho, Wan Yong Beh, Boon Kee Mohamed Alitheen, Noorjahan Banu |
| author_sort | Yeap, Swee Keong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The in vivo immunomodulatory effect of ethanolic extracts from leaves of Rhaphidophora korthalsii was determined via immune cell proliferation, T/NK cell phenotyping, and splenocyte cytotoxicity of BALB/c mice after 5 consecutive days of i.p. administration at various concentrations. Splenocyte proliferation index, cytotoxicity, peripheral blood T/NK cell population, and plasma cytokine (IL-2 and IFN-γ) in mice were assessed on day 5 and day 15. High concentration of extract (350μg/mice/day for 5 consecutive days) was able to stimulate immune cell proliferation, peripheral blood NK cell population, IL-2, and IFN-γ cytokines, as well as splenocyte cytotoxicity against Yac-1 cell line. Unlike rIL-2 which degraded rapidly, the stimulatory effect from the extract managed to last until day 15. These results suggested the potential of this extract as an alternative immunostimulator, and they encourage further study on guided fractionation and purification to identify the active ingredients that contribute to this in vitro and in vivo immunomodulatory activity. |
| first_indexed | 2025-11-15T08:38:59Z |
| format | Article |
| id | upm-24114 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:38:59Z |
| publishDate | 2012 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-241142015-10-27T03:24:53Z http://psasir.upm.edu.my/id/eprint/24114/ Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity. Yeap, Swee Keong Omar, Abdul Rahman Ali, Abdul Manaf Ho, Wan Yong Beh, Boon Kee Mohamed Alitheen, Noorjahan Banu The in vivo immunomodulatory effect of ethanolic extracts from leaves of Rhaphidophora korthalsii was determined via immune cell proliferation, T/NK cell phenotyping, and splenocyte cytotoxicity of BALB/c mice after 5 consecutive days of i.p. administration at various concentrations. Splenocyte proliferation index, cytotoxicity, peripheral blood T/NK cell population, and plasma cytokine (IL-2 and IFN-γ) in mice were assessed on day 5 and day 15. High concentration of extract (350μg/mice/day for 5 consecutive days) was able to stimulate immune cell proliferation, peripheral blood NK cell population, IL-2, and IFN-γ cytokines, as well as splenocyte cytotoxicity against Yac-1 cell line. Unlike rIL-2 which degraded rapidly, the stimulatory effect from the extract managed to last until day 15. These results suggested the potential of this extract as an alternative immunostimulator, and they encourage further study on guided fractionation and purification to identify the active ingredients that contribute to this in vitro and in vivo immunomodulatory activity. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24114/1/Immunomodulatory%20effect%20of%20Rhaphidophora%20korthalsii%20on%20natural%20killer%20cell%20cytotoxicity.pdf Yeap, Swee Keong and Omar, Abdul Rahman and Ali, Abdul Manaf and Ho, Wan Yong and Beh, Boon Kee and Mohamed Alitheen, Noorjahan Banu (2012) Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity. Evidence-Based Complementary and Alternative Medicine, 2012 (786487). pp. 1-7. ISSN 1741-427X; ESSN: 1741-4288 10.1155/2012/78648 English |
| spellingShingle | Yeap, Swee Keong Omar, Abdul Rahman Ali, Abdul Manaf Ho, Wan Yong Beh, Boon Kee Mohamed Alitheen, Noorjahan Banu Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity. |
| title | Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity. |
| title_full | Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity. |
| title_fullStr | Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity. |
| title_full_unstemmed | Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity. |
| title_short | Immunomodulatory effect of Rhaphidophora korthalsii on natural killer cell cytotoxicity. |
| title_sort | immunomodulatory effect of rhaphidophora korthalsii on natural killer cell cytotoxicity. |
| url | http://psasir.upm.edu.my/id/eprint/24114/ http://psasir.upm.edu.my/id/eprint/24114/ http://psasir.upm.edu.my/id/eprint/24114/1/Immunomodulatory%20effect%20of%20Rhaphidophora%20korthalsii%20on%20natural%20killer%20cell%20cytotoxicity.pdf |