Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression
Rhaphidophora korthalsii methanol extract was previously found as a potential in vitro immunomodulating agent. In this study, the in vitro immunomodulatory activity of R. korthalsii methanol extract on mice splenocyte were quantified through evaluating T/NK cell population, cytokine expression (IL-2...
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24113/ http://psasir.upm.edu.my/id/eprint/24113/1/24113.pdf |
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| author | Yeap, Swee Keong Omar, Abdul Rahman Ho, Wan Yong Beh, Boon Kee Ali, Abdul Manaf Mohammed Alitheen, Noorjahan Banu |
| author_facet | Yeap, Swee Keong Omar, Abdul Rahman Ho, Wan Yong Beh, Boon Kee Ali, Abdul Manaf Mohammed Alitheen, Noorjahan Banu |
| author_sort | Yeap, Swee Keong |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Rhaphidophora korthalsii methanol extract was previously found as a potential in vitro immunomodulating agent. In this study, the in vitro immunomodulatory activity of R. korthalsii methanol extract on mice splenocyte were quantified through evaluating T/NK cell population, cytokine expression (IL-2 and IFN-) and cytotoxicity against Yac-1 cell. In vitro MTT proliferation study showed that R. korthalsii methanol extract stimulate significant proliferation of splenocyte, thymocyte and bone marrow cell at 25 g/ml after 72 h incubation as compared to rIL-2. Besides, 25 g/ml of extract also enhanced T-helper 1 cytokine (IL-2 and IFN-) secretion, natural killer cell (NK1.1+CD3-) population and cytotoxicity against natural killer sensitive Yac-1 leukemic cell line. It was concluded that R. korthalsii methanol extract is a potential immunomodulator agent that could activate the natural killer cell. |
| first_indexed | 2025-11-15T08:38:59Z |
| format | Article |
| id | upm-24113 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:38:59Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-241132016-04-27T03:14:36Z http://psasir.upm.edu.my/id/eprint/24113/ Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression Yeap, Swee Keong Omar, Abdul Rahman Ho, Wan Yong Beh, Boon Kee Ali, Abdul Manaf Mohammed Alitheen, Noorjahan Banu Rhaphidophora korthalsii methanol extract was previously found as a potential in vitro immunomodulating agent. In this study, the in vitro immunomodulatory activity of R. korthalsii methanol extract on mice splenocyte were quantified through evaluating T/NK cell population, cytokine expression (IL-2 and IFN-) and cytotoxicity against Yac-1 cell. In vitro MTT proliferation study showed that R. korthalsii methanol extract stimulate significant proliferation of splenocyte, thymocyte and bone marrow cell at 25 g/ml after 72 h incubation as compared to rIL-2. Besides, 25 g/ml of extract also enhanced T-helper 1 cytokine (IL-2 and IFN-) secretion, natural killer cell (NK1.1+CD3-) population and cytotoxicity against natural killer sensitive Yac-1 leukemic cell line. It was concluded that R. korthalsii methanol extract is a potential immunomodulator agent that could activate the natural killer cell. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24113/1/24113.pdf Yeap, Swee Keong and Omar, Abdul Rahman and Ho, Wan Yong and Beh, Boon Kee and Ali, Abdul Manaf and Mohammed Alitheen, Noorjahan Banu (2011) Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression. African Journal of Biotechnology, 10 (52). art. no. E25A36A35246. pp. 10744-10751. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/E25A36A35246 |
| spellingShingle | Yeap, Swee Keong Omar, Abdul Rahman Ho, Wan Yong Beh, Boon Kee Ali, Abdul Manaf Mohammed Alitheen, Noorjahan Banu Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression |
| title | Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression |
| title_full | Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression |
| title_fullStr | Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression |
| title_full_unstemmed | Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression |
| title_short | Immunomodulatory effect of Rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression |
| title_sort | immunomodulatory effect of rhaphidophora korthalsii on mice splenocyte, thymocyte and bone marrow cell proliferation and cytokine expression |
| url | http://psasir.upm.edu.my/id/eprint/24113/ http://psasir.upm.edu.my/id/eprint/24113/ http://psasir.upm.edu.my/id/eprint/24113/1/24113.pdf |