Application of Fourier transform infrared (FT-IR) spectroscopy combined with chemometrics for authentication of cod-liver oil
Some vegetable oils such as canola (CaO), corn (CO), soybean (SO), and walnut (WO) oils have similar color with cod liver oil (CLO), therefore, the presence of these oils was difficult to detect using naked eye. For this reason, Fourier transform infrared (FTIR) spectroscopy using horizontal attenua...
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| Format: | Article |
| Language: | English |
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Elsevier
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24066/ http://psasir.upm.edu.my/id/eprint/24066/1/Application%20of%20Fourier%20transform%20infrared.pdf |
| _version_ | 1848844932783013888 |
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| author | Abdul Rohman, Che Man, Yaakob |
| author_facet | Abdul Rohman, Che Man, Yaakob |
| author_sort | Abdul Rohman, |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Some vegetable oils such as canola (CaO), corn (CO), soybean (SO), and walnut (WO) oils have similar color with cod liver oil (CLO), therefore, the presence of these oils was difficult to detect using naked eye. For this reason, Fourier transform infrared (FTIR) spectroscopy using horizontal attenuated total reflectance (HATR) as sampling accessory and in the combination with chemometrics was developed for detection and quantification of these vegetable oils as adulterants in CLO. The quantification of vegetable oils was carried out by using multivariate calibrations of partial least squares (PLS) and principle component regression (PCR), while the classification between pure CLO and CLOs adulterated with CaO, CO, SO, and WO was performed using discriminant analysis (DA). PLS with FTIR normal spectra was more suitable compared with PCR for quantification purposes with coefficient of determination (R2) higher than 0.99 and root mean square error of calibration (RMSEC) in the range of 0.04–0.82% (v/v). The PLS model was further used to predict the levels of these vegetable oils in independent samples for validation/prediction purpose. The root mean square error of prediction (RMSEP) values obtained were of 1.75% (v/v) (CaO), 1.39% (v/v) (CO), 1.35% (v/v) (SO), and 1.37% (v/v) (WO), respectively. The classification using DA revealed that the developed method can classify CLO and that mixed with these vegetable oils using 9 principal components. |
| first_indexed | 2025-11-15T08:38:47Z |
| format | Article |
| id | upm-24066 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:38:47Z |
| publishDate | 2011 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-240662017-10-31T08:34:42Z http://psasir.upm.edu.my/id/eprint/24066/ Application of Fourier transform infrared (FT-IR) spectroscopy combined with chemometrics for authentication of cod-liver oil Abdul Rohman, Che Man, Yaakob Some vegetable oils such as canola (CaO), corn (CO), soybean (SO), and walnut (WO) oils have similar color with cod liver oil (CLO), therefore, the presence of these oils was difficult to detect using naked eye. For this reason, Fourier transform infrared (FTIR) spectroscopy using horizontal attenuated total reflectance (HATR) as sampling accessory and in the combination with chemometrics was developed for detection and quantification of these vegetable oils as adulterants in CLO. The quantification of vegetable oils was carried out by using multivariate calibrations of partial least squares (PLS) and principle component regression (PCR), while the classification between pure CLO and CLOs adulterated with CaO, CO, SO, and WO was performed using discriminant analysis (DA). PLS with FTIR normal spectra was more suitable compared with PCR for quantification purposes with coefficient of determination (R2) higher than 0.99 and root mean square error of calibration (RMSEC) in the range of 0.04–0.82% (v/v). The PLS model was further used to predict the levels of these vegetable oils in independent samples for validation/prediction purpose. The root mean square error of prediction (RMSEP) values obtained were of 1.75% (v/v) (CaO), 1.39% (v/v) (CO), 1.35% (v/v) (SO), and 1.37% (v/v) (WO), respectively. The classification using DA revealed that the developed method can classify CLO and that mixed with these vegetable oils using 9 principal components. Elsevier 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24066/1/Application%20of%20Fourier%20transform%20infrared.pdf Abdul Rohman, and Che Man, Yaakob (2011) Application of Fourier transform infrared (FT-IR) spectroscopy combined with chemometrics for authentication of cod-liver oil. Vibrational Spectroscopy, 55 (2). pp. 141-145. ISSN 0924-2031; ESSN: 1873-3697 http://www.sciencedirect.com/science/article/pii/S0924203110001554?via%3Dihub#! 10.1016/j.vibspec.2010.10.001 |
| spellingShingle | Abdul Rohman, Che Man, Yaakob Application of Fourier transform infrared (FT-IR) spectroscopy combined with chemometrics for authentication of cod-liver oil |
| title | Application of Fourier transform infrared (FT-IR) spectroscopy combined with chemometrics for authentication of cod-liver oil |
| title_full | Application of Fourier transform infrared (FT-IR) spectroscopy combined with chemometrics for authentication of cod-liver oil |
| title_fullStr | Application of Fourier transform infrared (FT-IR) spectroscopy combined with chemometrics for authentication of cod-liver oil |
| title_full_unstemmed | Application of Fourier transform infrared (FT-IR) spectroscopy combined with chemometrics for authentication of cod-liver oil |
| title_short | Application of Fourier transform infrared (FT-IR) spectroscopy combined with chemometrics for authentication of cod-liver oil |
| title_sort | application of fourier transform infrared (ft-ir) spectroscopy combined with chemometrics for authentication of cod-liver oil |
| url | http://psasir.upm.edu.my/id/eprint/24066/ http://psasir.upm.edu.my/id/eprint/24066/ http://psasir.upm.edu.my/id/eprint/24066/ http://psasir.upm.edu.my/id/eprint/24066/1/Application%20of%20Fourier%20transform%20infrared.pdf |