Quantitative analysis of Roundup Ready soybean content in soy-derived food and animal feed by using Real-time PCR incorporated with cloned DNA fragments
Malaysia, Biosafety Bill 2006 was approved by Parliament in July 2007, and labeling legislation will be implemented soon. In this study, duplex polymerase chain reaction (PCR) was carried out to detect endogenous soybean lectin gene and exogenous cp4-epsps (5’-enolpyruvylshikimate-3-phospate syn...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24061/ http://psasir.upm.edu.my/id/eprint/24061/1/24061.pdf |
| _version_ | 1848844931477536768 |
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| author | Khoo, Siew Ping Cheah, Yoke Kqueen Radu, Son |
| author_facet | Khoo, Siew Ping Cheah, Yoke Kqueen Radu, Son |
| author_sort | Khoo, Siew Ping |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Malaysia, Biosafety Bill 2006 was approved by Parliament in July 2007, and labeling legislation
will be implemented soon. In this study, duplex polymerase chain reaction (PCR) was carried out to detect
endogenous soybean lectin gene and exogenous cp4-epsps (5’-enolpyruvylshikimate-3-phospate synthase)
gene simultaneously. Additionally, real-time PCR utilizing SYBR Green fluorescence dye were established for
the quantitative analysis of Roundup Ready soybean (RRS), which is based on the two established calibration
curve from cloned fragment of cp4-epsps gene and lectin gene respectively. Approximately, 39.5% (45/114)
of the samples examined in this study contain RRS, animal feeds (31), processed food (13) and raw soybean
(1). Additionally, 75.6% (34/45) of the positive samples were found contained RRS above 0.9%. The sensitive
GMO quantitative approach described in this study enable the analysis of various samples and this will facilitate
the labeling process. |
| first_indexed | 2025-11-15T08:38:46Z |
| format | Article |
| id | upm-24061 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:38:46Z |
| publishDate | 2011 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-240612015-06-02T02:44:12Z http://psasir.upm.edu.my/id/eprint/24061/ Quantitative analysis of Roundup Ready soybean content in soy-derived food and animal feed by using Real-time PCR incorporated with cloned DNA fragments Khoo, Siew Ping Cheah, Yoke Kqueen Radu, Son Malaysia, Biosafety Bill 2006 was approved by Parliament in July 2007, and labeling legislation will be implemented soon. In this study, duplex polymerase chain reaction (PCR) was carried out to detect endogenous soybean lectin gene and exogenous cp4-epsps (5’-enolpyruvylshikimate-3-phospate synthase) gene simultaneously. Additionally, real-time PCR utilizing SYBR Green fluorescence dye were established for the quantitative analysis of Roundup Ready soybean (RRS), which is based on the two established calibration curve from cloned fragment of cp4-epsps gene and lectin gene respectively. Approximately, 39.5% (45/114) of the samples examined in this study contain RRS, animal feeds (31), processed food (13) and raw soybean (1). Additionally, 75.6% (34/45) of the positive samples were found contained RRS above 0.9%. The sensitive GMO quantitative approach described in this study enable the analysis of various samples and this will facilitate the labeling process. Faculty of Food Science and Technology, Universiti Putra Malaysia 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24061/1/24061.pdf Khoo, Siew Ping and Cheah, Yoke Kqueen and Radu, Son (2011) Quantitative analysis of Roundup Ready soybean content in soy-derived food and animal feed by using Real-time PCR incorporated with cloned DNA fragments. International Food Research Journal, 18 (2). pp. 507-514. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-18-2011.html http://www.ifrj.upm.edu.my/18%20(02)%202011/(6)%20IFRJ-2010-108.pdf English |
| spellingShingle | Khoo, Siew Ping Cheah, Yoke Kqueen Radu, Son Quantitative analysis of Roundup Ready soybean content in soy-derived food and animal feed by using Real-time PCR incorporated with cloned DNA fragments |
| title | Quantitative analysis of Roundup Ready soybean content in
soy-derived food and animal feed by using Real-time PCR
incorporated with cloned DNA fragments |
| title_full | Quantitative analysis of Roundup Ready soybean content in
soy-derived food and animal feed by using Real-time PCR
incorporated with cloned DNA fragments |
| title_fullStr | Quantitative analysis of Roundup Ready soybean content in
soy-derived food and animal feed by using Real-time PCR
incorporated with cloned DNA fragments |
| title_full_unstemmed | Quantitative analysis of Roundup Ready soybean content in
soy-derived food and animal feed by using Real-time PCR
incorporated with cloned DNA fragments |
| title_short | Quantitative analysis of Roundup Ready soybean content in
soy-derived food and animal feed by using Real-time PCR
incorporated with cloned DNA fragments |
| title_sort | quantitative analysis of roundup ready soybean content in
soy-derived food and animal feed by using real-time pcr
incorporated with cloned dna fragments |
| url | http://psasir.upm.edu.my/id/eprint/24061/ http://psasir.upm.edu.my/id/eprint/24061/ http://psasir.upm.edu.my/id/eprint/24061/ http://psasir.upm.edu.my/id/eprint/24061/1/24061.pdf |