Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia
Hepatitis A is a liver infection caused by the hepatitis A virus (HAV). Outbreaks of hepatitis A have been linked to the consumption of both raw and cooked shellfish. These outbreaks could induce a public confidence problem over shellfish safety and may result in important economic losses for th...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Faculty of Food Science and Technology, Universiti Putra Malaysia
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24050/ http://psasir.upm.edu.my/id/eprint/24050/1/24050.pdf |
| _version_ | 1848844928528941056 |
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| author | Md. Nordin, Wan Norhana A. Rahim, Masazurah Mahyudin, Nor Ainy I., Ismail |
| author_facet | Md. Nordin, Wan Norhana A. Rahim, Masazurah Mahyudin, Nor Ainy I., Ismail |
| author_sort | Md. Nordin, Wan Norhana |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Hepatitis A is a liver infection caused by the hepatitis A virus (HAV). Outbreaks of hepatitis A
have been linked to the consumption of both raw and cooked shellfish. These outbreaks could induce a public
confidence problem over shellfish safety and may result in important economic losses for the seafood industry.
The work presented in this study investigated the presence of HAV in shellfish from Peninsular Malaysia. A
total of 365 of cultured and wild shellfish from 36 sampling locations located throughout Peninsular Malaysia
were examined using a commercial nucleic acid extraction and reverse transcription -polymerase chain reaction
(RT-PCR) kit. HAV was not detected in almost all of the shellfish samples examined. Only one cockle sample
from Changkat, Seberang Perai was positive for HAV. The results suggest the absence of HAV or very low
amount of HAV viral particles in most of the shellfish examined. |
| first_indexed | 2025-11-15T08:38:43Z |
| format | Article |
| id | upm-24050 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:38:43Z |
| publishDate | 2011 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-240502015-06-02T08:23:05Z http://psasir.upm.edu.my/id/eprint/24050/ Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia Md. Nordin, Wan Norhana A. Rahim, Masazurah Mahyudin, Nor Ainy I., Ismail Hepatitis A is a liver infection caused by the hepatitis A virus (HAV). Outbreaks of hepatitis A have been linked to the consumption of both raw and cooked shellfish. These outbreaks could induce a public confidence problem over shellfish safety and may result in important economic losses for the seafood industry. The work presented in this study investigated the presence of HAV in shellfish from Peninsular Malaysia. A total of 365 of cultured and wild shellfish from 36 sampling locations located throughout Peninsular Malaysia were examined using a commercial nucleic acid extraction and reverse transcription -polymerase chain reaction (RT-PCR) kit. HAV was not detected in almost all of the shellfish samples examined. Only one cockle sample from Changkat, Seberang Perai was positive for HAV. The results suggest the absence of HAV or very low amount of HAV viral particles in most of the shellfish examined. Faculty of Food Science and Technology, Universiti Putra Malaysia 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24050/1/24050.pdf Md. Nordin, Wan Norhana and A. Rahim, Masazurah and Mahyudin, Nor Ainy and I., Ismail (2011) Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia. International Food Research Journal, 18 (1). pp. 411-415. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-18-2011.html English |
| spellingShingle | Md. Nordin, Wan Norhana A. Rahim, Masazurah Mahyudin, Nor Ainy I., Ismail Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia |
| title | Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia |
| title_full | Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia |
| title_fullStr | Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia |
| title_full_unstemmed | Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia |
| title_short | Reverse Transcriptase PCR detection of Hepatitis A virus (HAV) in cultured and wild shellfish from the Peninsular of Malaysia |
| title_sort | reverse transcriptase pcr detection of hepatitis a virus (hav) in cultured and wild shellfish from the peninsular of malaysia |
| url | http://psasir.upm.edu.my/id/eprint/24050/ http://psasir.upm.edu.my/id/eprint/24050/ http://psasir.upm.edu.my/id/eprint/24050/1/24050.pdf |