Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level
Little is known on the biosafety level of Vibrio spp. in freshwater fish in Malaysia. The purpose of this study was to investigate the prevalence and concentration of Vibrio spp. and V. parahaemolyticus in freshwater fish using the Most Probable Number-Polymerase Chain Reaction (MPN-PCR) method. The...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Food Science and Technology, Universiti Putra Malaysia
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/24038/ http://psasir.upm.edu.my/id/eprint/24038/1/24038.pdf |
| _version_ | 1848844925173497856 |
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| author | Ahmad, Noorlis Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Tuan Chilek, Tuan Zainazor Ponniah, Jeyaletchumi Robin, Tunung Tang, John Yew Huat Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Radu, Son |
| author_facet | Ahmad, Noorlis Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Tuan Chilek, Tuan Zainazor Ponniah, Jeyaletchumi Robin, Tunung Tang, John Yew Huat Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Radu, Son |
| author_sort | Ahmad, Noorlis |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Little is known on the biosafety level of Vibrio spp. in freshwater fish in Malaysia. The purpose of this study was to investigate the prevalence and concentration of Vibrio spp. and V. parahaemolyticus in freshwater fish using the Most Probable Number-Polymerase Chain Reaction (MPN-PCR) method. The study was conducted on 150 samples from two types of freshwater fish commonly sold at hypermarkets, i.e. Pangasius hypophthalmus (catfish) and Oreochromis sp. (red tilapia). Sampling was done on the flesh, intestinal tract and gills of each fish. The prevalence of Vibrio spp. and V. parahaemolyticus was found to be 98.67% and 24% respectively with higher percentages detected in samples from the gills followed by the intestinal tract and flesh. Vibrio spp. was detected in almost all red tilapia and catfish samples. V. parahaemolyticus was detected in 25% of the catfish samples compared to 22.6% of red tilapia fish. The density of Vibrio spp. and V. parahaemolyticus in the samples ranged from 0 to 1.1x107 MPN/g. Although the maximum value was 1.1x107 MPN/g, most samples had microbial loads ranging from 0 to >104 MPN/g. The outcome on the biosafety assessment of Vibrio spp. and V. parahaemolyticus in freshwater fish indicates another potential source of food safety issues to consumers.
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| first_indexed | 2025-11-15T08:38:40Z |
| format | Article |
| id | upm-24038 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:38:40Z |
| publishDate | 2011 |
| publisher | Faculty of Food Science and Technology, Universiti Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-240382015-06-01T06:01:44Z http://psasir.upm.edu.my/id/eprint/24038/ Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level Ahmad, Noorlis Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Tuan Chilek, Tuan Zainazor Ponniah, Jeyaletchumi Robin, Tunung Tang, John Yew Huat Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Radu, Son Little is known on the biosafety level of Vibrio spp. in freshwater fish in Malaysia. The purpose of this study was to investigate the prevalence and concentration of Vibrio spp. and V. parahaemolyticus in freshwater fish using the Most Probable Number-Polymerase Chain Reaction (MPN-PCR) method. The study was conducted on 150 samples from two types of freshwater fish commonly sold at hypermarkets, i.e. Pangasius hypophthalmus (catfish) and Oreochromis sp. (red tilapia). Sampling was done on the flesh, intestinal tract and gills of each fish. The prevalence of Vibrio spp. and V. parahaemolyticus was found to be 98.67% and 24% respectively with higher percentages detected in samples from the gills followed by the intestinal tract and flesh. Vibrio spp. was detected in almost all red tilapia and catfish samples. V. parahaemolyticus was detected in 25% of the catfish samples compared to 22.6% of red tilapia fish. The density of Vibrio spp. and V. parahaemolyticus in the samples ranged from 0 to 1.1x107 MPN/g. Although the maximum value was 1.1x107 MPN/g, most samples had microbial loads ranging from 0 to >104 MPN/g. The outcome on the biosafety assessment of Vibrio spp. and V. parahaemolyticus in freshwater fish indicates another potential source of food safety issues to consumers. Faculty of Food Science and Technology, Universiti Putra Malaysia 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/24038/1/24038.pdf Ahmad, Noorlis and Mohamad Ghazali, Farinazleen and Cheah, Yoke Kqueen and Tuan Chilek, Tuan Zainazor and Ponniah, Jeyaletchumi and Robin, Tunung and Tang, John Yew Huat and Nishibuchi, Mitsuaki and Nakaguchi, Yoshitsugu and Radu, Son (2011) Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level. International Food Research Journal, 18 (2). pp. 689-695. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/volume-18-2011.html |
| spellingShingle | Ahmad, Noorlis Mohamad Ghazali, Farinazleen Cheah, Yoke Kqueen Tuan Chilek, Tuan Zainazor Ponniah, Jeyaletchumi Robin, Tunung Tang, John Yew Huat Nishibuchi, Mitsuaki Nakaguchi, Yoshitsugu Radu, Son Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level |
| title | Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level |
| title_full | Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level |
| title_fullStr | Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level |
| title_full_unstemmed | Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level |
| title_short | Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freshwater fish at hypermarket level |
| title_sort | prevalence and quantification of vibrio species and vibrio parahaemolyticus in freshwater fish at hypermarket level |
| url | http://psasir.upm.edu.my/id/eprint/24038/ http://psasir.upm.edu.my/id/eprint/24038/ http://psasir.upm.edu.my/id/eprint/24038/1/24038.pdf |