Biomodification of Kenaf using white rot fungi.

White rot fungi can be used as a pretreatment of biomass to degrade lignin. It also alters the structure of the lignocellulosic matter, thus increasing its accessibility to enzymes able to convert polysaccharides into simple sugars. This study compares the ability of two species of white rot fungi,...

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Main Authors: Halis, Rasmina, Tan, Hui Rus, Ashaari, Zaidon, Mohamed, Rozi
Format: Article
Language:English
English
Published: North Carolina State University 2012
Online Access:http://psasir.upm.edu.my/id/eprint/23993/
http://psasir.upm.edu.my/id/eprint/23993/1/Biomodification%20of%20Kenaf%20using%20white%20rot%20fungi.pdf
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author Halis, Rasmina
Tan, Hui Rus
Ashaari, Zaidon
Mohamed, Rozi
author_facet Halis, Rasmina
Tan, Hui Rus
Ashaari, Zaidon
Mohamed, Rozi
author_sort Halis, Rasmina
building UPM Institutional Repository
collection Online Access
description White rot fungi can be used as a pretreatment of biomass to degrade lignin. It also alters the structure of the lignocellulosic matter, thus increasing its accessibility to enzymes able to convert polysaccharides into simple sugars. This study compares the ability of two species of white rot fungi, Pycnoporous sanguineus and Oxyporus latemarginatus FRIM 31, to degrade lignin in kenaf chips. The white rot fungi were originally isolated from the tropical forest in Malaysia. Kenaf chips were first inoculated with each fungus separately using corn steep liquor as a fungal growth promoter. The kenaf chips were inoculated with white rot fungus for a period of 1, 2, 4, 8 and 16 weeks, after which they were observed under the scanning electron microscope (SEM). Chemical analyses were conducted following TAPPI Standard Methods and Fourier Transmission Infra Red (FTIR). SEM observations showed evidence of fungal colonization. When calculating weight loss, both P. sanguineus and O. latemarginatus FRIM 31 showed the greatest reduction. Amounts by mass of cellulose, hemicelluloses, extractives, and lignin in the treated kenaf chips all were lowered. The results show that O. latemarginatus FRIM 31 had a greater ability to degrade lignin when compared to P. sanguineus.
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spelling upm-239932015-09-13T23:59:53Z http://psasir.upm.edu.my/id/eprint/23993/ Biomodification of Kenaf using white rot fungi. Halis, Rasmina Tan, Hui Rus Ashaari, Zaidon Mohamed, Rozi White rot fungi can be used as a pretreatment of biomass to degrade lignin. It also alters the structure of the lignocellulosic matter, thus increasing its accessibility to enzymes able to convert polysaccharides into simple sugars. This study compares the ability of two species of white rot fungi, Pycnoporous sanguineus and Oxyporus latemarginatus FRIM 31, to degrade lignin in kenaf chips. The white rot fungi were originally isolated from the tropical forest in Malaysia. Kenaf chips were first inoculated with each fungus separately using corn steep liquor as a fungal growth promoter. The kenaf chips were inoculated with white rot fungus for a period of 1, 2, 4, 8 and 16 weeks, after which they were observed under the scanning electron microscope (SEM). Chemical analyses were conducted following TAPPI Standard Methods and Fourier Transmission Infra Red (FTIR). SEM observations showed evidence of fungal colonization. When calculating weight loss, both P. sanguineus and O. latemarginatus FRIM 31 showed the greatest reduction. Amounts by mass of cellulose, hemicelluloses, extractives, and lignin in the treated kenaf chips all were lowered. The results show that O. latemarginatus FRIM 31 had a greater ability to degrade lignin when compared to P. sanguineus. North Carolina State University 2012-02 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23993/1/Biomodification%20of%20Kenaf%20using%20white%20rot%20fungi.pdf Halis, Rasmina and Tan, Hui Rus and Ashaari, Zaidon and Mohamed, Rozi (2012) Biomodification of Kenaf using white rot fungi. BioResources, 7 (1). pp. 984-996. ISSN 1930-2126 http://www.ncsu.edu/‎ English
spellingShingle Halis, Rasmina
Tan, Hui Rus
Ashaari, Zaidon
Mohamed, Rozi
Biomodification of Kenaf using white rot fungi.
title Biomodification of Kenaf using white rot fungi.
title_full Biomodification of Kenaf using white rot fungi.
title_fullStr Biomodification of Kenaf using white rot fungi.
title_full_unstemmed Biomodification of Kenaf using white rot fungi.
title_short Biomodification of Kenaf using white rot fungi.
title_sort biomodification of kenaf using white rot fungi.
url http://psasir.upm.edu.my/id/eprint/23993/
http://psasir.upm.edu.my/id/eprint/23993/
http://psasir.upm.edu.my/id/eprint/23993/1/Biomodification%20of%20Kenaf%20using%20white%20rot%20fungi.pdf