Estrogen-induced vitellogenin in Tor tambroides (Bleeker, 1854): purification, characterization and ELISA development.
Vitellogenin (vtg) is a high molecular weight glycophospholipoprotein synthesized in the liver under stimulation of estrogen. Basically found in sexually mature female, vtg being taken up by developing oocyte during maturation. It functioned as a nutrient storage for growing embryo. Having potential...
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| Format: | Article |
| Language: | English English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23817/ http://psasir.upm.edu.my/id/eprint/23817/1/Estrogen.pdf |
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| author | Muhammad, Nurul Ashikin Christianus, Annie Daud, Siti Khalijah Saad, Che Roos Harmin, Sharr Azni Md Yasin, Ina Salwany |
| author_facet | Muhammad, Nurul Ashikin Christianus, Annie Daud, Siti Khalijah Saad, Che Roos Harmin, Sharr Azni Md Yasin, Ina Salwany |
| author_sort | Muhammad, Nurul Ashikin |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Vitellogenin (vtg) is a high molecular weight glycophospholipoprotein synthesized in the liver under stimulation of estrogen. Basically found in sexually mature female, vtg being taken up by developing oocyte during maturation. It functioned as a nutrient storage for growing embryo. Having potential to be used as a maturation indicator, vtg helps to enhance fish fry production. Tor tambroides is one of the most sought after fish in Malaysia for having potential as a game fish yet so important economically due to high demand by farmers as well as consumers. Main problem in the mass production of T. tambroides fry in hatchery is in the availability and selection of matured broodstock. Identification of matured and ready females morphologically can only be done by experience workers. Therefore, establishment of simple technique to definite identify matured females is necessary. This has led to the identification, purification and development of enzyme linked immunosorbent assay (ELISA) to measure blood plasma vtg as maturation indicator. This will definitely contribute to the hatchery production of T. tambroides fry. |
| first_indexed | 2025-11-15T08:37:41Z |
| format | Article |
| id | upm-23817 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:37:41Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-238172015-10-19T23:39:22Z http://psasir.upm.edu.my/id/eprint/23817/ Estrogen-induced vitellogenin in Tor tambroides (Bleeker, 1854): purification, characterization and ELISA development. Muhammad, Nurul Ashikin Christianus, Annie Daud, Siti Khalijah Saad, Che Roos Harmin, Sharr Azni Md Yasin, Ina Salwany Vitellogenin (vtg) is a high molecular weight glycophospholipoprotein synthesized in the liver under stimulation of estrogen. Basically found in sexually mature female, vtg being taken up by developing oocyte during maturation. It functioned as a nutrient storage for growing embryo. Having potential to be used as a maturation indicator, vtg helps to enhance fish fry production. Tor tambroides is one of the most sought after fish in Malaysia for having potential as a game fish yet so important economically due to high demand by farmers as well as consumers. Main problem in the mass production of T. tambroides fry in hatchery is in the availability and selection of matured broodstock. Identification of matured and ready females morphologically can only be done by experience workers. Therefore, establishment of simple technique to definite identify matured females is necessary. This has led to the identification, purification and development of enzyme linked immunosorbent assay (ELISA) to measure blood plasma vtg as maturation indicator. This will definitely contribute to the hatchery production of T. tambroides fry. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23817/1/Estrogen.pdf Muhammad, Nurul Ashikin and Christianus, Annie and Daud, Siti Khalijah and Saad, Che Roos and Harmin, Sharr Azni and Md Yasin, Ina Salwany (2011) Estrogen-induced vitellogenin in Tor tambroides (Bleeker, 1854): purification, characterization and ELISA development. Journal of Fisheries and Aquatic Science, 6 (7). pp. 700-714. ISSN 1816-4927; ESSN: 1996-0751 10.3923/jfas.2011.700.714 English |
| spellingShingle | Muhammad, Nurul Ashikin Christianus, Annie Daud, Siti Khalijah Saad, Che Roos Harmin, Sharr Azni Md Yasin, Ina Salwany Estrogen-induced vitellogenin in Tor tambroides (Bleeker, 1854): purification, characterization and ELISA development. |
| title | Estrogen-induced vitellogenin in Tor tambroides (Bleeker, 1854): purification, characterization and ELISA development. |
| title_full | Estrogen-induced vitellogenin in Tor tambroides (Bleeker, 1854): purification, characterization and ELISA development. |
| title_fullStr | Estrogen-induced vitellogenin in Tor tambroides (Bleeker, 1854): purification, characterization and ELISA development. |
| title_full_unstemmed | Estrogen-induced vitellogenin in Tor tambroides (Bleeker, 1854): purification, characterization and ELISA development. |
| title_short | Estrogen-induced vitellogenin in Tor tambroides (Bleeker, 1854): purification, characterization and ELISA development. |
| title_sort | estrogen-induced vitellogenin in tor tambroides (bleeker, 1854): purification, characterization and elisa development. |
| url | http://psasir.upm.edu.my/id/eprint/23817/ http://psasir.upm.edu.my/id/eprint/23817/ http://psasir.upm.edu.my/id/eprint/23817/1/Estrogen.pdf |