Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers.
Direct amplified length polymorphism (DALP) combines the advantages of a high-resolution fingerprint method and also characterizing the genetic polymorphisms. This molecular method was also found to be useful in brown planthopper, Nilaparvata lugens species complex for the analysis of genetic polymo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23789/ http://psasir.upm.edu.my/id/eprint/23789/1/Genetic%20dissection%20of%20sympatric%20populations%20of%20brown%20planthopper.pdf |
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| author | Abdul Latif, Mohammad Yusop, Mohd Rafii Mazid, Muhammad S. Ali, Mussa E. Ahmed, Faheem Omar, Mohd Yosuh Tan, Soon Guan |
| author_facet | Abdul Latif, Mohammad Yusop, Mohd Rafii Mazid, Muhammad S. Ali, Mussa E. Ahmed, Faheem Omar, Mohd Yosuh Tan, Soon Guan |
| author_sort | Abdul Latif, Mohammad |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Direct amplified length polymorphism (DALP) combines the advantages of a high-resolution fingerprint method and also characterizing the genetic polymorphisms. This molecular method was also found to be useful in brown planthopper, Nilaparvata lugens species complex for the analysis of genetic polymorphisms. A total of 11 populations of Nilaparvata spp. were collected from 6 locations from Malaysia. Two sympatric populations of brown planthopper, N. lugens, one from rice and the other from a weed grass (Leersia hexandra), were collected from each of five locations. N. bakeri was used as an out group. Three oligonucleotide primer pairs, DALP231/DALPR′5, DALP234/DALPR′5, and DALP235/DALPR′5 were applied in this study. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on genetic distances for the 11 populations of Nilaparvata spp. revealed that populations belonging to the same species and the same host type clustered together irrespective of their geographical localities of capture. The populations of N. lugens formed into two distinct clusters, one was insects with high esterase activities usually captured from rice and the other was with low esterase activities usually captured from L. hexandra. N. bakeri, an out group, was the most isolated group. Analyses of principal components, molecular variance, and robustness also supported greatly to the findings of cluster analysis. |
| first_indexed | 2025-11-15T08:37:34Z |
| format | Article |
| id | upm-23789 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:37:34Z |
| publishDate | 2012 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-237892016-02-10T01:20:24Z http://psasir.upm.edu.my/id/eprint/23789/ Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers. Abdul Latif, Mohammad Yusop, Mohd Rafii Mazid, Muhammad S. Ali, Mussa E. Ahmed, Faheem Omar, Mohd Yosuh Tan, Soon Guan Direct amplified length polymorphism (DALP) combines the advantages of a high-resolution fingerprint method and also characterizing the genetic polymorphisms. This molecular method was also found to be useful in brown planthopper, Nilaparvata lugens species complex for the analysis of genetic polymorphisms. A total of 11 populations of Nilaparvata spp. were collected from 6 locations from Malaysia. Two sympatric populations of brown planthopper, N. lugens, one from rice and the other from a weed grass (Leersia hexandra), were collected from each of five locations. N. bakeri was used as an out group. Three oligonucleotide primer pairs, DALP231/DALPR′5, DALP234/DALPR′5, and DALP235/DALPR′5 were applied in this study. The unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on genetic distances for the 11 populations of Nilaparvata spp. revealed that populations belonging to the same species and the same host type clustered together irrespective of their geographical localities of capture. The populations of N. lugens formed into two distinct clusters, one was insects with high esterase activities usually captured from rice and the other was with low esterase activities usually captured from L. hexandra. N. bakeri, an out group, was the most isolated group. Analyses of principal components, molecular variance, and robustness also supported greatly to the findings of cluster analysis. Hindawi Publishing Corporation 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23789/1/Genetic%20dissection%20of%20sympatric%20populations%20of%20brown%20planthopper.pdf Abdul Latif, Mohammad and Yusop, Mohd Rafii and Mazid, Muhammad S. and Ali, Mussa E. and Ahmed, Faheem and Omar, Mohd Yosuh and Tan, Soon Guan (2012) Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers. The Scientific World Journal, 12. pp. 1-11. ISSN 1537-744X 10.1100/2012/586831 |
| spellingShingle | Abdul Latif, Mohammad Yusop, Mohd Rafii Mazid, Muhammad S. Ali, Mussa E. Ahmed, Faheem Omar, Mohd Yosuh Tan, Soon Guan Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers. |
| title | Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers. |
| title_full | Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers. |
| title_fullStr | Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers. |
| title_full_unstemmed | Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers. |
| title_short | Genetic dissection of sympatric populations of brown planthopper, Nilaparvata lugens (Stl), using DALP-PCR molecular markers. |
| title_sort | genetic dissection of sympatric populations of brown planthopper, nilaparvata lugens (stl), using dalp-pcr molecular markers. |
| url | http://psasir.upm.edu.my/id/eprint/23789/ http://psasir.upm.edu.my/id/eprint/23789/ http://psasir.upm.edu.my/id/eprint/23789/1/Genetic%20dissection%20of%20sympatric%20populations%20of%20brown%20planthopper.pdf |