Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos.
Purpose To investigate the effect of epigenetic modification on pattern, time and capacity of transcription activation of POU5F1, the key marker of pluripotency, in cloned bovine embryos. Methods Bovine fibroblasts were stably transfected with POU5F1 promoter-driven enhanced green fluorescent p...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Springer
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23763/ http://psasir.upm.edu.my/id/eprint/23763/1/Epigenetic%20modification%20does%20not%20determine%20the%20time%20of%20POU5F1%20transcription%20activation%20in%20cloned%20bovine%20embryos.pdf |
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| author | Jafari, Shahram Hosseini, Sayyed Morteza Hajian, Mehdi Forouzanfar, Mohsen Jafarpour, Farnoosh Abedi, Parvaneh Ostadhosseini, Somayyeh Abbasi, Hassan Gourabi, Hamid Shahverdi, Abdolhossein H. Vosough, Ahmad Dizaj Anjomshoaa, Maryam Haron, Abd Wahid Nordin, Norshariza Yaakub, Halimatun Nasr Esfahani, Mohammad Hosein |
| author_facet | Jafari, Shahram Hosseini, Sayyed Morteza Hajian, Mehdi Forouzanfar, Mohsen Jafarpour, Farnoosh Abedi, Parvaneh Ostadhosseini, Somayyeh Abbasi, Hassan Gourabi, Hamid Shahverdi, Abdolhossein H. Vosough, Ahmad Dizaj Anjomshoaa, Maryam Haron, Abd Wahid Nordin, Norshariza Yaakub, Halimatun Nasr Esfahani, Mohammad Hosein |
| author_sort | Jafari, Shahram |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Purpose
To investigate the effect of epigenetic modification on pattern, time and capacity of transcription activation of POU5F1, the key marker of pluripotency, in cloned bovine embryos.
Methods
Bovine fibroblasts were stably transfected with POU5F1 promoter-driven enhanced green fluorescent protein (EGFP). This provided a visible marker to investigate the effect of post-activation treatment of cloned bovine embryos with trichostatin A (TSA) on time and capacity of POU5F1 expression and its subsequent effect on in vitro development of cloned bovine embryos.
Results
Irrespective of TSA treatment, POU5F1 expression was not detected until 8–16 cell stage, but was detected in both inner cell mass and trophectoderm at the blastocyst stage. TSA treatment significantly increased POU5F1 expression, and the yield and quality of cloned embryo development compared to control.
Conclusion
The POU5F1 expression of cloned embryos is strictly controlled by the stage of embryo development and may not be altered by TSA-mediated changes occur in DNA-methylation and histone-acetylation of the genome. |
| first_indexed | 2025-11-15T08:37:27Z |
| format | Article |
| id | upm-23763 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:37:27Z |
| publishDate | 2011 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-237632015-10-06T07:56:45Z http://psasir.upm.edu.my/id/eprint/23763/ Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos. Jafari, Shahram Hosseini, Sayyed Morteza Hajian, Mehdi Forouzanfar, Mohsen Jafarpour, Farnoosh Abedi, Parvaneh Ostadhosseini, Somayyeh Abbasi, Hassan Gourabi, Hamid Shahverdi, Abdolhossein H. Vosough, Ahmad Dizaj Anjomshoaa, Maryam Haron, Abd Wahid Nordin, Norshariza Yaakub, Halimatun Nasr Esfahani, Mohammad Hosein Purpose To investigate the effect of epigenetic modification on pattern, time and capacity of transcription activation of POU5F1, the key marker of pluripotency, in cloned bovine embryos. Methods Bovine fibroblasts were stably transfected with POU5F1 promoter-driven enhanced green fluorescent protein (EGFP). This provided a visible marker to investigate the effect of post-activation treatment of cloned bovine embryos with trichostatin A (TSA) on time and capacity of POU5F1 expression and its subsequent effect on in vitro development of cloned bovine embryos. Results Irrespective of TSA treatment, POU5F1 expression was not detected until 8–16 cell stage, but was detected in both inner cell mass and trophectoderm at the blastocyst stage. TSA treatment significantly increased POU5F1 expression, and the yield and quality of cloned embryo development compared to control. Conclusion The POU5F1 expression of cloned embryos is strictly controlled by the stage of embryo development and may not be altered by TSA-mediated changes occur in DNA-methylation and histone-acetylation of the genome. Springer 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23763/1/Epigenetic%20modification%20does%20not%20determine%20the%20time%20of%20POU5F1%20transcription%20activation%20in%20cloned%20bovine%20embryos.pdf Jafari, Shahram and Hosseini, Sayyed Morteza and Hajian, Mehdi and Forouzanfar, Mohsen and Jafarpour, Farnoosh and Abedi, Parvaneh and Ostadhosseini, Somayyeh and Abbasi, Hassan and Gourabi, Hamid and Shahverdi, Abdolhossein H. and Vosough, Ahmad Dizaj and Anjomshoaa, Maryam and Haron, Abd Wahid and Nordin, Norshariza and Yaakub, Halimatun and Nasr Esfahani, Mohammad Hosein (2011) Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos. Journal of Assisted Reproduction and Genetics, 28 (11). 1119 - 1127. ISSN 1058-0468; ESSN: 1573-7330 10.1007/s10815-011-9638-1 English |
| spellingShingle | Jafari, Shahram Hosseini, Sayyed Morteza Hajian, Mehdi Forouzanfar, Mohsen Jafarpour, Farnoosh Abedi, Parvaneh Ostadhosseini, Somayyeh Abbasi, Hassan Gourabi, Hamid Shahverdi, Abdolhossein H. Vosough, Ahmad Dizaj Anjomshoaa, Maryam Haron, Abd Wahid Nordin, Norshariza Yaakub, Halimatun Nasr Esfahani, Mohammad Hosein Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos. |
| title | Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos. |
| title_full | Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos. |
| title_fullStr | Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos. |
| title_full_unstemmed | Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos. |
| title_short | Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos. |
| title_sort | epigenetic modification does not determine the time of pou5f1 transcription activation in cloned bovine embryos. |
| url | http://psasir.upm.edu.my/id/eprint/23763/ http://psasir.upm.edu.my/id/eprint/23763/ http://psasir.upm.edu.my/id/eprint/23763/1/Epigenetic%20modification%20does%20not%20determine%20the%20time%20of%20POU5F1%20transcription%20activation%20in%20cloned%20bovine%20embryos.pdf |