Huanglongbing detection, gene sequencing and cell wall thickness modification in infected Citrus reticulata after chemical treatments.
Huanglongbing (HLB), also known as the citrus greening disease, is a destructive disease that has affected citrus plants in more than 40 countries worldwide. HLB is very difficult to control when the vector is spread in the citriculture areas. As presently there is no effective treatment for the cit...
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| Format: | Article |
| Language: | English English |
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CurrentSciDirect Publications
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23728/ http://psasir.upm.edu.my/id/eprint/23728/1/Huanglongbing%20detection.pdf |
| _version_ | 1848844839474429952 |
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| author | Haji Vand, Shokrollah Abdullah, Thohirah Lee Sijam, Kamaruzaman |
| author_facet | Haji Vand, Shokrollah Abdullah, Thohirah Lee Sijam, Kamaruzaman |
| author_sort | Haji Vand, Shokrollah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Huanglongbing (HLB), also known as the citrus greening disease, is a destructive disease that has affected citrus plants in more than 40 countries worldwide. HLB is very difficult to control when the vector is spread in the citriculture areas. As presently there is no effective treatment for the citrus plants infected by the HLB disease, this study investigates chemical treatments
using GA3, an antibiotic (Oxi-tetracycline), a foliar fertilizer and a combination of these chemicals to stimulate resistance to HLB in citrus plants. It employs a RCBD design with three replications. Aqueous solutions of the treatments were prepared and sprayed on whole trees
and a small dose of antibiotic was injected into the trunks of the trees. Conventional PCR was not able to detect HLB bacteria in T7 but it was detected in all treatments using second conventional PCR. A comparison of the mean thickness of treated plants showed that thick cell
walls were found in T4 and T7. A new strain of HLB was identified and registered in the NCBI
gene bank database (GU133055) from Terengganu. |
| first_indexed | 2025-11-15T08:37:18Z |
| format | Article |
| id | upm-23728 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:37:18Z |
| publishDate | 2011 |
| publisher | CurrentSciDirect Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-237282015-09-14T02:48:22Z http://psasir.upm.edu.my/id/eprint/23728/ Huanglongbing detection, gene sequencing and cell wall thickness modification in infected Citrus reticulata after chemical treatments. Haji Vand, Shokrollah Abdullah, Thohirah Lee Sijam, Kamaruzaman Huanglongbing (HLB), also known as the citrus greening disease, is a destructive disease that has affected citrus plants in more than 40 countries worldwide. HLB is very difficult to control when the vector is spread in the citriculture areas. As presently there is no effective treatment for the citrus plants infected by the HLB disease, this study investigates chemical treatments using GA3, an antibiotic (Oxi-tetracycline), a foliar fertilizer and a combination of these chemicals to stimulate resistance to HLB in citrus plants. It employs a RCBD design with three replications. Aqueous solutions of the treatments were prepared and sprayed on whole trees and a small dose of antibiotic was injected into the trunks of the trees. Conventional PCR was not able to detect HLB bacteria in T7 but it was detected in all treatments using second conventional PCR. A comparison of the mean thickness of treated plants showed that thick cell walls were found in T4 and T7. A new strain of HLB was identified and registered in the NCBI gene bank database (GU133055) from Terengganu. CurrentSciDirect Publications 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23728/1/Huanglongbing%20detection.pdf Haji Vand, Shokrollah and Abdullah, Thohirah Lee and Sijam, Kamaruzaman (2011) Huanglongbing detection, gene sequencing and cell wall thickness modification in infected Citrus reticulata after chemical treatments. International Journal of Current Scientific Research, 1 (4). pp. 177-182. ISSN 2231-6276 English |
| spellingShingle | Haji Vand, Shokrollah Abdullah, Thohirah Lee Sijam, Kamaruzaman Huanglongbing detection, gene sequencing and cell wall thickness modification in infected Citrus reticulata after chemical treatments. |
| title | Huanglongbing detection, gene sequencing and cell wall thickness modification in infected Citrus reticulata after chemical treatments. |
| title_full | Huanglongbing detection, gene sequencing and cell wall thickness modification in infected Citrus reticulata after chemical treatments. |
| title_fullStr | Huanglongbing detection, gene sequencing and cell wall thickness modification in infected Citrus reticulata after chemical treatments. |
| title_full_unstemmed | Huanglongbing detection, gene sequencing and cell wall thickness modification in infected Citrus reticulata after chemical treatments. |
| title_short | Huanglongbing detection, gene sequencing and cell wall thickness modification in infected Citrus reticulata after chemical treatments. |
| title_sort | huanglongbing detection, gene sequencing and cell wall thickness modification in infected citrus reticulata after chemical treatments. |
| url | http://psasir.upm.edu.my/id/eprint/23728/ http://psasir.upm.edu.my/id/eprint/23728/1/Huanglongbing%20detection.pdf |