Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant
A comparative phenotypic and morpho-histological study was carried out on tissue culture-derived truncated leaf syndrome (TLS) and wild-type oil palm seedlings to investigate their phenotypic and morpho-histological differences. On the basis of the percentage of TLS occurence in a clone, the TLS see...
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| Format: | Article |
| Language: | English |
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Springer
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23683/ http://psasir.upm.edu.my/id/eprint/23683/1/23683.pdf |
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| author | Habib, Sheikh Hasna Syed Alwee, Sharifah Shahrul Rabiah Ho, Chai Ling Abdullah, Meilina Ong Sinniah, Uma Rani Namasivayam, Parameswari |
| author_facet | Habib, Sheikh Hasna Syed Alwee, Sharifah Shahrul Rabiah Ho, Chai Ling Abdullah, Meilina Ong Sinniah, Uma Rani Namasivayam, Parameswari |
| author_sort | Habib, Sheikh Hasna |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A comparative phenotypic and morpho-histological study was carried out on tissue culture-derived truncated leaf syndrome (TLS) and wild-type oil palm seedlings to investigate their phenotypic and morpho-histological differences. On the basis of the percentage of TLS occurence in a clone, the TLS seedlings were categorized into three groups: severe (70–100%), moderate (40–69%) and mild (<40%). Wild and TLS seedlings differ in terms of growth, vigor, leaf size and shape, root number, volume, length as well as the size of shoot apical meristem (SAM). Differences were also found in fresh weight of leaf, root and SAM of TLS in comparison to wild-type seedlings. Depressed and wavy leaf surface, sunken and distorted stomata and coalesced epidermal cells were observed by scanning electron microscopy in TLS seedlings. The size, shape and number of stomata were also different in the TLS leaf compared to the wild type. Longer epidermal cells, depressed epidermal layer, larger sub-epidermal cells and loosely arranged less mesophyll cells were observed in TLS leaf than in wild type. Undifferentiated vascular bundle was found in TLS leaves where metaxylem and phloem were absent and root tips were impaired. The size and leaf primordial arrangement of SAM were remarkably different in TLS compared to wild-type seedlings suggesting that these alterations might be due to smaller SAM. Therefore, further detailed genetic analysis on TLS SAM is needed for clear understanding of TLS occurrence. |
| first_indexed | 2025-11-15T08:37:06Z |
| format | Article |
| id | upm-23683 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:37:06Z |
| publishDate | 2012 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-236832019-10-23T00:50:04Z http://psasir.upm.edu.my/id/eprint/23683/ Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant Habib, Sheikh Hasna Syed Alwee, Sharifah Shahrul Rabiah Ho, Chai Ling Abdullah, Meilina Ong Sinniah, Uma Rani Namasivayam, Parameswari A comparative phenotypic and morpho-histological study was carried out on tissue culture-derived truncated leaf syndrome (TLS) and wild-type oil palm seedlings to investigate their phenotypic and morpho-histological differences. On the basis of the percentage of TLS occurence in a clone, the TLS seedlings were categorized into three groups: severe (70–100%), moderate (40–69%) and mild (<40%). Wild and TLS seedlings differ in terms of growth, vigor, leaf size and shape, root number, volume, length as well as the size of shoot apical meristem (SAM). Differences were also found in fresh weight of leaf, root and SAM of TLS in comparison to wild-type seedlings. Depressed and wavy leaf surface, sunken and distorted stomata and coalesced epidermal cells were observed by scanning electron microscopy in TLS seedlings. The size, shape and number of stomata were also different in the TLS leaf compared to the wild type. Longer epidermal cells, depressed epidermal layer, larger sub-epidermal cells and loosely arranged less mesophyll cells were observed in TLS leaf than in wild type. Undifferentiated vascular bundle was found in TLS leaves where metaxylem and phloem were absent and root tips were impaired. The size and leaf primordial arrangement of SAM were remarkably different in TLS compared to wild-type seedlings suggesting that these alterations might be due to smaller SAM. Therefore, further detailed genetic analysis on TLS SAM is needed for clear understanding of TLS occurrence. Springer 2012 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/23683/1/23683.pdf Habib, Sheikh Hasna and Syed Alwee, Sharifah Shahrul Rabiah and Ho, Chai Ling and Abdullah, Meilina Ong and Sinniah, Uma Rani and Namasivayam, Parameswari (2012) Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant. Acta Physiologiae Plantarum, 34 (1). pp. 17-28. ISSN 0137-5881; ESSN: 1861-1664 https://link.springer.com/article/10.1007%2Fs11738-011-0800-2 10.1007/s11738-011-0800-2 |
| spellingShingle | Habib, Sheikh Hasna Syed Alwee, Sharifah Shahrul Rabiah Ho, Chai Ling Abdullah, Meilina Ong Sinniah, Uma Rani Namasivayam, Parameswari Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant |
| title | Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant |
| title_full | Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant |
| title_fullStr | Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant |
| title_full_unstemmed | Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant |
| title_short | Morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (E. guineensis Jacq.) somaclonal variant |
| title_sort | morpho-histological characterization of truncated leaf syndrome seedlings: an oil palm (e. guineensis jacq.) somaclonal variant |
| url | http://psasir.upm.edu.my/id/eprint/23683/ http://psasir.upm.edu.my/id/eprint/23683/ http://psasir.upm.edu.my/id/eprint/23683/ http://psasir.upm.edu.my/id/eprint/23683/1/23683.pdf |