Preliminary analysis of cryopreservation of Dendrobium Bobby Messina orchid using an encapsulation-dehydration technique with Evans blue assay
In vitro grown protocorm-like bodies (PLBs) of Dendrobium Bobby Messina hybrid were cryopreserved in liquid nitrogen (LN) at -196°C by an encapsulation-dehydration technique. PLBs (1 to 2 and 3 to 4 mm) were precultured in half strength semi-solid MS media supplemented with six different concentrati...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2011
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/23681/ http://psasir.upm.edu.my/id/eprint/23681/1/23681.pdf |
| _version_ | 1848844826434338816 |
|---|---|
| author | Zainuddin, Masitah Julkifle, Advina Lizah Pobathy, Ranjetta Sinniah, Uma Rani Khoddamzadeh, Amir James Antony, Jessica Jeyanthi Pavallekoodi, Subramaniam, Sreeramanan |
| author_facet | Zainuddin, Masitah Julkifle, Advina Lizah Pobathy, Ranjetta Sinniah, Uma Rani Khoddamzadeh, Amir James Antony, Jessica Jeyanthi Pavallekoodi, Subramaniam, Sreeramanan |
| author_sort | Zainuddin, Masitah |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | In vitro grown protocorm-like bodies (PLBs) of Dendrobium Bobby Messina hybrid were cryopreserved in liquid nitrogen (LN) at -196°C by an encapsulation-dehydration technique. PLBs (1 to 2 and 3 to 4 mm) were precultured in half strength semi-solid MS media supplemented with six different concentrations of sucrose (0.0, 0.2, 0.4, 0.6, 0.8 and 1.0 M). The PLBs were then encapsulated to form the beads in halfstrength liquid MS media supplemented with different concentrations of sodium alginate (2.5, 3.0 and 3.5%). The beads were placed in 2 ml cryovials and plunged into LN for 24 h. The beads were then thawed in a 40°C water bath for 90 s and were placed in recovery media composed of half strength semisolid MS media supplemented with 2% sucrose for four days under dark condition. After 12 days, the Evans blue dye assay was carried out to determine the viability of the PLBs. The highest viability was found in 1 to 2mm PLBs precultured in half strength semi-solid MS media supplemented with 1.0 M sucrose and encapsulated in 2.5% sodium alginate. Biochemical content analyses (chlorophyll, total soluble protein and peroxidase activities) were done to investigate the physiological responses of the PLBs after cryopreservation. |
| first_indexed | 2025-11-15T08:37:05Z |
| format | Article |
| id | upm-23681 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:37:05Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-236812016-04-27T02:05:06Z http://psasir.upm.edu.my/id/eprint/23681/ Preliminary analysis of cryopreservation of Dendrobium Bobby Messina orchid using an encapsulation-dehydration technique with Evans blue assay Zainuddin, Masitah Julkifle, Advina Lizah Pobathy, Ranjetta Sinniah, Uma Rani Khoddamzadeh, Amir James Antony, Jessica Jeyanthi Pavallekoodi, Subramaniam, Sreeramanan In vitro grown protocorm-like bodies (PLBs) of Dendrobium Bobby Messina hybrid were cryopreserved in liquid nitrogen (LN) at -196°C by an encapsulation-dehydration technique. PLBs (1 to 2 and 3 to 4 mm) were precultured in half strength semi-solid MS media supplemented with six different concentrations of sucrose (0.0, 0.2, 0.4, 0.6, 0.8 and 1.0 M). The PLBs were then encapsulated to form the beads in halfstrength liquid MS media supplemented with different concentrations of sodium alginate (2.5, 3.0 and 3.5%). The beads were placed in 2 ml cryovials and plunged into LN for 24 h. The beads were then thawed in a 40°C water bath for 90 s and were placed in recovery media composed of half strength semisolid MS media supplemented with 2% sucrose for four days under dark condition. After 12 days, the Evans blue dye assay was carried out to determine the viability of the PLBs. The highest viability was found in 1 to 2mm PLBs precultured in half strength semi-solid MS media supplemented with 1.0 M sucrose and encapsulated in 2.5% sodium alginate. Biochemical content analyses (chlorophyll, total soluble protein and peroxidase activities) were done to investigate the physiological responses of the PLBs after cryopreservation. Academic Journals 2011-09-26 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23681/1/23681.pdf Zainuddin, Masitah and Julkifle, Advina Lizah and Pobathy, Ranjetta and Sinniah, Uma Rani and Khoddamzadeh, Amir and James Antony, Jessica Jeyanthi and Pavallekoodi, and Subramaniam, Sreeramanan (2011) Preliminary analysis of cryopreservation of Dendrobium Bobby Messina orchid using an encapsulation-dehydration technique with Evans blue assay. African Journal of Biotechnology, 10 (56). art. no. 75981B137092. pp. 11870-11878. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/75981B137092 |
| spellingShingle | Zainuddin, Masitah Julkifle, Advina Lizah Pobathy, Ranjetta Sinniah, Uma Rani Khoddamzadeh, Amir James Antony, Jessica Jeyanthi Pavallekoodi, Subramaniam, Sreeramanan Preliminary analysis of cryopreservation of Dendrobium Bobby Messina orchid using an encapsulation-dehydration technique with Evans blue assay |
| title | Preliminary analysis of cryopreservation of Dendrobium Bobby Messina orchid using an encapsulation-dehydration technique with Evans blue assay |
| title_full | Preliminary analysis of cryopreservation of Dendrobium Bobby Messina orchid using an encapsulation-dehydration technique with Evans blue assay |
| title_fullStr | Preliminary analysis of cryopreservation of Dendrobium Bobby Messina orchid using an encapsulation-dehydration technique with Evans blue assay |
| title_full_unstemmed | Preliminary analysis of cryopreservation of Dendrobium Bobby Messina orchid using an encapsulation-dehydration technique with Evans blue assay |
| title_short | Preliminary analysis of cryopreservation of Dendrobium Bobby Messina orchid using an encapsulation-dehydration technique with Evans blue assay |
| title_sort | preliminary analysis of cryopreservation of dendrobium bobby messina orchid using an encapsulation-dehydration technique with evans blue assay |
| url | http://psasir.upm.edu.my/id/eprint/23681/ http://psasir.upm.edu.my/id/eprint/23681/ http://psasir.upm.edu.my/id/eprint/23681/1/23681.pdf |