Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa).
Among 120 simple sequence repeat (SSR) markers, 23 polymorphic markers were used to identify the segregation ratio in 320 individuals of an F(2) rice population derived from Pongsu Seribu 2, a resistant variety, and Mahsuri, a susceptible rice cultivar. For phenotypic study, the most virulent blast...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English |
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2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23616/ http://psasir.upm.edu.my/id/eprint/23616/1/Analysis%20of%20simple%20sequence%20repeat%20markers%20linked%20with%20blast%20disease%20resistance%20genes%20in%20a%20segregating%20population%20of%20rice.pdf |
| _version_ | 1848844807898660864 |
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| author | S., Ashkani Yusop, Mohd Rafii Meon, Sariah Abdullah, Siti Nor Akmar H., Rusli H., Abdul Rahim Latif, M.A. |
| author_facet | S., Ashkani Yusop, Mohd Rafii Meon, Sariah Abdullah, Siti Nor Akmar H., Rusli H., Abdul Rahim Latif, M.A. |
| author_sort | S., Ashkani |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Among 120 simple sequence repeat (SSR) markers, 23 polymorphic markers were used to identify the segregation ratio in 320 individuals of an F(2) rice population derived from Pongsu Seribu 2, a resistant variety, and Mahsuri, a susceptible rice cultivar. For phenotypic study, the most virulent blast (Magnaporthe oryzae) pathotype, P7.2, was used in screening of F(2) population in order to understand the inheritance of blast resistance as well as linkage with SSR markers. Only 11 markers showed a good fit to the expected segregation ratio (1:2:1) for the single gene model (d.f. = 1.0, P < 0.05) in chi-square (χ(2)) analyses. In the phenotypic data analysis, the F(2) population segregated in a 3:1 (R:S) ratio for resistant and susceptible plants, respectively. Therefore, resistance to blast pathotype P7.2 in Pongsu Seribu 2 is most likely controlled by a single nuclear gene. The plants from F(2) lines that showed resistance to blast pathotype P7.2 were linked to six alleles of SSR markers, RM168 (116 bp), RM8225 (221 bp), RM1233 (175 bp), RM6836 (240 bp), RM5961 (129 bp), and RM413 (79 bp). These diagnostic markers could be used in marker assisted selection programs to develop a durable blast resistant variety. |
| first_indexed | 2025-11-15T08:36:48Z |
| format | Article |
| id | upm-23616 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:36:48Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-236162016-02-18T05:12:07Z http://psasir.upm.edu.my/id/eprint/23616/ Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa). S., Ashkani Yusop, Mohd Rafii Meon, Sariah Abdullah, Siti Nor Akmar H., Rusli H., Abdul Rahim Latif, M.A. Among 120 simple sequence repeat (SSR) markers, 23 polymorphic markers were used to identify the segregation ratio in 320 individuals of an F(2) rice population derived from Pongsu Seribu 2, a resistant variety, and Mahsuri, a susceptible rice cultivar. For phenotypic study, the most virulent blast (Magnaporthe oryzae) pathotype, P7.2, was used in screening of F(2) population in order to understand the inheritance of blast resistance as well as linkage with SSR markers. Only 11 markers showed a good fit to the expected segregation ratio (1:2:1) for the single gene model (d.f. = 1.0, P < 0.05) in chi-square (χ(2)) analyses. In the phenotypic data analysis, the F(2) population segregated in a 3:1 (R:S) ratio for resistant and susceptible plants, respectively. Therefore, resistance to blast pathotype P7.2 in Pongsu Seribu 2 is most likely controlled by a single nuclear gene. The plants from F(2) lines that showed resistance to blast pathotype P7.2 were linked to six alleles of SSR markers, RM168 (116 bp), RM8225 (221 bp), RM1233 (175 bp), RM6836 (240 bp), RM5961 (129 bp), and RM413 (79 bp). These diagnostic markers could be used in marker assisted selection programs to develop a durable blast resistant variety. 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23616/1/Analysis%20of%20simple%20sequence%20repeat%20markers%20linked%20with%20blast%20disease%20resistance%20genes%20in%20a%20segregating%20population%20of%20rice.pdf S., Ashkani and Yusop, Mohd Rafii and Meon, Sariah and Abdullah, Siti Nor Akmar and H., Rusli and H., Abdul Rahim and Latif, M.A. (2011) Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa). Genetics and Molecular Research, 10 (3). pp. 1345-1355. ISSN 1676-5680 10.4238/vol10-3gmr1331 English |
| spellingShingle | S., Ashkani Yusop, Mohd Rafii Meon, Sariah Abdullah, Siti Nor Akmar H., Rusli H., Abdul Rahim Latif, M.A. Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa). |
| title | Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa). |
| title_full | Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa). |
| title_fullStr | Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa). |
| title_full_unstemmed | Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa). |
| title_short | Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa). |
| title_sort | analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (oryza sativa). |
| url | http://psasir.upm.edu.my/id/eprint/23616/ http://psasir.upm.edu.my/id/eprint/23616/ http://psasir.upm.edu.my/id/eprint/23616/1/Analysis%20of%20simple%20sequence%20repeat%20markers%20linked%20with%20blast%20disease%20resistance%20genes%20in%20a%20segregating%20population%20of%20rice.pdf |