Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper.
Three emission wavelengths from two-photon excitation techniques were use to determinefree Ca2+ using a new analytical calculation. This was achieved by considering theinteraction of protein with free Ca2+ and indo-1 in protein contaminated sample. The emissions obtained from the excitation with the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23367/ http://psasir.upm.edu.my/id/eprint/23367/2/Using%20two.pdf |
| _version_ | 1848844738402189312 |
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| author | Muhammad Noor, Ahmad Shukri Inami, W. Miyakawa, Atsuo Kawata, Yoshimasa |
| author_facet | Muhammad Noor, Ahmad Shukri Inami, W. Miyakawa, Atsuo Kawata, Yoshimasa |
| author_sort | Muhammad Noor, Ahmad Shukri |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Three emission wavelengths from two-photon excitation techniques were use to determinefree Ca2+ using a new analytical calculation. This was achieved by considering theinteraction of protein with free Ca2+ and indo-1 in protein contaminated sample. The emissions obtained from the excitation with the dissociation constants were used in the calculation. Agreement values of the Ca2+ from the calculation with the known Ca2+ buffer solution used for the measurement was obtained for various Ca2+ and protein concentration. The analytical analysis shows that dissociation constants for each binding’sdeviation errors are small to affect free Ca2+ determination. This would provide an accurate measurement of free Ca2+ in biological cells than the typical two emission wavelength processes. Furthermore, using two-photon excitation methods, deep inside cells observation of free Ca2+ are possible using the methods introduced. |
| first_indexed | 2025-11-15T08:35:41Z |
| format | Article |
| id | upm-23367 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:35:41Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-233672015-10-20T05:59:36Z http://psasir.upm.edu.my/id/eprint/23367/ Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper. Muhammad Noor, Ahmad Shukri Inami, W. Miyakawa, Atsuo Kawata, Yoshimasa Three emission wavelengths from two-photon excitation techniques were use to determinefree Ca2+ using a new analytical calculation. This was achieved by considering theinteraction of protein with free Ca2+ and indo-1 in protein contaminated sample. The emissions obtained from the excitation with the dissociation constants were used in the calculation. Agreement values of the Ca2+ from the calculation with the known Ca2+ buffer solution used for the measurement was obtained for various Ca2+ and protein concentration. The analytical analysis shows that dissociation constants for each binding’sdeviation errors are small to affect free Ca2+ determination. This would provide an accurate measurement of free Ca2+ in biological cells than the typical two emission wavelength processes. Furthermore, using two-photon excitation methods, deep inside cells observation of free Ca2+ are possible using the methods introduced. 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23367/2/Using%20two.pdf Muhammad Noor, Ahmad Shukri and Inami, W. and Miyakawa, Atsuo and Kawata, Yoshimasa (2011) Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper. International Journal of the Physical Sciences, 6 (33). art. no. D06240B19089 . 7435 -7440. ISSN 1992 -1950 10.5897/IJPS11.482 English |
| spellingShingle | Muhammad Noor, Ahmad Shukri Inami, W. Miyakawa, Atsuo Kawata, Yoshimasa Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper. |
| title | Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper. |
| title_full | Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper. |
| title_fullStr | Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper. |
| title_full_unstemmed | Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper. |
| title_short | Using two-photon excitation methods for determination of Ca2+ in contamination with protein - full length research paper. |
| title_sort | using two-photon excitation methods for determination of ca2+ in contamination with protein - full length research paper. |
| url | http://psasir.upm.edu.my/id/eprint/23367/ http://psasir.upm.edu.my/id/eprint/23367/ http://psasir.upm.edu.my/id/eprint/23367/2/Using%20two.pdf |