Production and purification of the phosphoprotein of Nipah virus in Escherichia coli for use in diagnostic assays.
ABSTRACT Nipah Virus (NiV) is an emerging zoonotic paramyxovirus that can be fatal in humans and various types of animals. The phospho (P) protein of NiV plays an important role in RNA synthesis, replication, and genome synthesis. In this study, the NiV P gene was cloned into a pTrcHis2-TOPO vect...
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| Format: | Article |
| Language: | English |
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2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/23239/ http://psasir.upm.edu.my/id/eprint/23239/1/Production%20and%20purification%20of%20the%20phosphoprotein%20of%20Nipah%20virus%20in%20Escherichia%20coli%20for%20use%20in%20diagnostic%20assays.pdf |
| _version_ | 1848844701588783104 |
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| author | Tan, Wen Siang Tey, Beng Ti Salvamani, Shamala Wen, Cheng Ng |
| author_facet | Tan, Wen Siang Tey, Beng Ti Salvamani, Shamala Wen, Cheng Ng |
| author_sort | Tan, Wen Siang |
| building | UPM Institutional Repository |
| collection | Online Access |
| description |
ABSTRACT
Nipah Virus (NiV) is an emerging zoonotic paramyxovirus that can be fatal in humans and various types of animals. The phospho (P) protein of NiV plays an important role in RNA synthesis, replication, and genome synthesis. In this study, the NiV P gene was cloned into a pTrcHis2-TOPO vector and the recombinant protein containing a His-tag was produced in Escherichia coli. SDS-PAGE and Western blot analysis using the anti-His antibody confirmed the protein expression. An optimization study of E. coli fermentation showed that the optimal cultivation temperature was 37°C, while the optimal induction time for P protein expression was at 9 h with 1 mM IPTG. Solubility analysis showed that E. coli cultivated at 37°C produced the highest fraction (70%) of soluble P protein. The recombinant P protein was purified from clarified E. coli lysate using an immobilized metal affinity chromatography (IMAC) technique to a purity of 92.67%, with a purification factor of 11.58. The purified P protein strongly reacted with the anti-NiV swine sera collected during a NiV outbreak, suggesting its potential as a diagnostic reagent. |
| first_indexed | 2025-11-15T08:35:06Z |
| format | Article |
| id | upm-23239 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:35:06Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-232392016-01-12T04:42:17Z http://psasir.upm.edu.my/id/eprint/23239/ Production and purification of the phosphoprotein of Nipah virus in Escherichia coli for use in diagnostic assays. Tan, Wen Siang Tey, Beng Ti Salvamani, Shamala Wen, Cheng Ng ABSTRACT Nipah Virus (NiV) is an emerging zoonotic paramyxovirus that can be fatal in humans and various types of animals. The phospho (P) protein of NiV plays an important role in RNA synthesis, replication, and genome synthesis. In this study, the NiV P gene was cloned into a pTrcHis2-TOPO vector and the recombinant protein containing a His-tag was produced in Escherichia coli. SDS-PAGE and Western blot analysis using the anti-His antibody confirmed the protein expression. An optimization study of E. coli fermentation showed that the optimal cultivation temperature was 37°C, while the optimal induction time for P protein expression was at 9 h with 1 mM IPTG. Solubility analysis showed that E. coli cultivated at 37°C produced the highest fraction (70%) of soluble P protein. The recombinant P protein was purified from clarified E. coli lysate using an immobilized metal affinity chromatography (IMAC) technique to a purity of 92.67%, with a purification factor of 11.58. The purified P protein strongly reacted with the anti-NiV swine sera collected during a NiV outbreak, suggesting its potential as a diagnostic reagent. 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/23239/1/Production%20and%20purification%20of%20the%20phosphoprotein%20of%20Nipah%20virus%20in%20Escherichia%20coli%20for%20use%20in%20diagnostic%20assays.pdf Tan, Wen Siang and Tey, Beng Ti and Salvamani, Shamala and Wen, Cheng Ng (2011) Production and purification of the phosphoprotein of Nipah virus in Escherichia coli for use in diagnostic assays. Biotechnology and Bioprocess Engineering, 16 (6). pp. 1166-1172. ISSN 1226-8372 10.1007/s12257-011-0095-6 |
| spellingShingle | Tan, Wen Siang Tey, Beng Ti Salvamani, Shamala Wen, Cheng Ng Production and purification of the phosphoprotein of Nipah virus in Escherichia coli for use in diagnostic assays. |
| title | Production and purification of the phosphoprotein of Nipah virus in Escherichia coli for use in diagnostic assays. |
| title_full | Production and purification of the phosphoprotein of Nipah virus in Escherichia coli for use in diagnostic assays. |
| title_fullStr | Production and purification of the phosphoprotein of Nipah virus in Escherichia coli for use in diagnostic assays. |
| title_full_unstemmed | Production and purification of the phosphoprotein of Nipah virus in Escherichia coli for use in diagnostic assays. |
| title_short | Production and purification of the phosphoprotein of Nipah virus in Escherichia coli for use in diagnostic assays. |
| title_sort | production and purification of the phosphoprotein of nipah virus in escherichia coli for use in diagnostic assays. |
| url | http://psasir.upm.edu.my/id/eprint/23239/ http://psasir.upm.edu.my/id/eprint/23239/ http://psasir.upm.edu.my/id/eprint/23239/1/Production%20and%20purification%20of%20the%20phosphoprotein%20of%20Nipah%20virus%20in%20Escherichia%20coli%20for%20use%20in%20diagnostic%20assays.pdf |