Optimization of a native gel electrophoretic process for the purification of intracellular green fluorescent protein from intact Escherichia coli cells

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Optimization of a native gel electrophoretic process for the purification of intracellular green fluorescent protein from intact Escherichia coli cells

Intracellular green fluorescent protein (GFP) can be separated and purified from intact Escherichia coli cells by a preparative native polyacrylamide gel electrophoresis (n-PAGE). The effects of operating parameters such as the volume and concentration of feedstock and the pore size and height of re...

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Bibliographic Details
Main Authors:	Chew, Few Ne, Tan, Wen Siang, Ling, Tau Chuan, Tey, Beng Ti
Format:	Article
Language:	English
Published:	Elsevier 2011
Online Access:	http://psasir.upm.edu.my/id/eprint/23105/ http://psasir.upm.edu.my/id/eprint/23105/1/Optimization%20of%20a%20native%20gel%20electrophoretic%20process%20for%20the%20purification%20of%20intracellular%20green%20fluorescent%20protein%20from%20intact%20Escherichia%20coli%20cells.pdf

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