Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis

White root disease caused by Rigidoporus microporus is the most devastating disease in majority of the rubber growing countries, including Malaysia. This study aimed to screen and decipher the mechanisms involved in the biocontrol agents responsible for the inhibition of R. microporus. Among 16 fung...

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Main Authors: Go, Wen Ze, H'ng, Paik San, Wong, Mui Yun, Chin, Kit Ling, Ujang, Salmiah, Noran, Aizat Shamin
Format: Article
Language:English
Published: Informa UK 2019
Online Access:http://psasir.upm.edu.my/id/eprint/22670/
http://psasir.upm.edu.my/id/eprint/22670/1/Evaluation%20of%20Trichoderma%20asperellum%20as%20a%20potential%20biocontrol%20agent%20against%20Rigidoporus%20microporus%20Hevea%20brasiliensis.pdf
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author Go, Wen Ze
H'ng, Paik San
Wong, Mui Yun
Chin, Kit Ling
Ujang, Salmiah
Noran, Aizat Shamin
author_facet Go, Wen Ze
H'ng, Paik San
Wong, Mui Yun
Chin, Kit Ling
Ujang, Salmiah
Noran, Aizat Shamin
author_sort Go, Wen Ze
building UPM Institutional Repository
collection Online Access
description White root disease caused by Rigidoporus microporus is the most devastating disease in majority of the rubber growing countries, including Malaysia. This study aimed to screen and decipher the mechanisms involved in the biocontrol agents responsible for the inhibition of R. microporus. Among 16 fungal isolates, Trichoderma spp. showed promising results with the highest percent of inhibition shown by Trichoderma asperellum (80.54%). Scanning electron microscopy study revealed coiling of hyphae by Trichoderma species against R. microporus. T. asperellum has demonstrated a maximum inhibition in both volatile and non-volatile metabolite tests with its 75% culture filtrate on PDA plate was observed to cause abnormal morphological character in R. microporus. All Trichoderma species were shown to produce hydrolytic enzymes (chitinase, cellulase and β-1,3-glucanase) and they were active siderophore producers. Present study demonstrated the possible mechanisms involved and responsible for successful inhibition of R. microporus under in vitro condition especially by T. asperellum.
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spelling upm-226702020-03-05T06:33:12Z http://psasir.upm.edu.my/id/eprint/22670/ Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis Go, Wen Ze H'ng, Paik San Wong, Mui Yun Chin, Kit Ling Ujang, Salmiah Noran, Aizat Shamin White root disease caused by Rigidoporus microporus is the most devastating disease in majority of the rubber growing countries, including Malaysia. This study aimed to screen and decipher the mechanisms involved in the biocontrol agents responsible for the inhibition of R. microporus. Among 16 fungal isolates, Trichoderma spp. showed promising results with the highest percent of inhibition shown by Trichoderma asperellum (80.54%). Scanning electron microscopy study revealed coiling of hyphae by Trichoderma species against R. microporus. T. asperellum has demonstrated a maximum inhibition in both volatile and non-volatile metabolite tests with its 75% culture filtrate on PDA plate was observed to cause abnormal morphological character in R. microporus. All Trichoderma species were shown to produce hydrolytic enzymes (chitinase, cellulase and β-1,3-glucanase) and they were active siderophore producers. Present study demonstrated the possible mechanisms involved and responsible for successful inhibition of R. microporus under in vitro condition especially by T. asperellum. Informa UK 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/22670/1/Evaluation%20of%20Trichoderma%20asperellum%20as%20a%20potential%20biocontrol%20agent%20against%20Rigidoporus%20microporus%20Hevea%20brasiliensis.pdf Go, Wen Ze and H'ng, Paik San and Wong, Mui Yun and Chin, Kit Ling and Ujang, Salmiah and Noran, Aizat Shamin (2019) Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis. Archives of Phytopathology and Plant Protection, 52 (7-8). pp. 639-666. ISSN 0323-5408; ESSN: 1477-2906 https://www.tandfonline.com/doi/abs/10.1080/03235408.2019.1587821 10.1080/03235408.2019.1587821
spellingShingle Go, Wen Ze
H'ng, Paik San
Wong, Mui Yun
Chin, Kit Ling
Ujang, Salmiah
Noran, Aizat Shamin
Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis
title Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis
title_full Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis
title_fullStr Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis
title_full_unstemmed Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis
title_short Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis
title_sort evaluation of trichoderma asperellum as a potential biocontrol agent against rigidoporus microporus hevea brasiliensis
url http://psasir.upm.edu.my/id/eprint/22670/
http://psasir.upm.edu.my/id/eprint/22670/
http://psasir.upm.edu.my/id/eprint/22670/
http://psasir.upm.edu.my/id/eprint/22670/1/Evaluation%20of%20Trichoderma%20asperellum%20as%20a%20potential%20biocontrol%20agent%20against%20Rigidoporus%20microporus%20Hevea%20brasiliensis.pdf