A non-dominated sorting Differential Search Algorithm Flux Balance Analysis (ndsDSAFBA) for in silico multiobjective optimization in identifying reactions knockout

Metabolic engineering is defined as improving the cellular activities of an organism by manipulating the metabolic, signal or regulatory network. In silico reaction knockout simulation is one of the techniques applied to analyse the effects of genetic perturbations on metabolite production. Many met...

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Main Authors: Mohd Daud, Kauthar, Mohamad, Mohd Saberi, Zakaria, Zalmiyah, Hassan, Rohayanti, Ali Shah, Zuraini, Deris, Safa'ai, Ibrahim, Zuwairie, Napis, Suhaimi, Sinnott, Richard O.
Format: Article
Language:English
Published: Elsevier 2019
Online Access:http://psasir.upm.edu.my/id/eprint/22593/
http://psasir.upm.edu.my/id/eprint/22593/1/A%20non-dominated%20sorting%20Differential%20Search%20Algorithm%20Flux%20Balance%20Analysis%20%28ndsDSAFBA%29%20for%20in%20silico%20multiobjective%20optimization%20in%20identifying%20reactions%20knockout.pdf
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author Mohd Daud, Kauthar
Mohamad, Mohd Saberi
Zakaria, Zalmiyah
Hassan, Rohayanti
Ali Shah, Zuraini
Deris, Safa'ai
Ibrahim, Zuwairie
Napis, Suhaimi
Sinnott, Richard O.
author_facet Mohd Daud, Kauthar
Mohamad, Mohd Saberi
Zakaria, Zalmiyah
Hassan, Rohayanti
Ali Shah, Zuraini
Deris, Safa'ai
Ibrahim, Zuwairie
Napis, Suhaimi
Sinnott, Richard O.
author_sort Mohd Daud, Kauthar
building UPM Institutional Repository
collection Online Access
description Metabolic engineering is defined as improving the cellular activities of an organism by manipulating the metabolic, signal or regulatory network. In silico reaction knockout simulation is one of the techniques applied to analyse the effects of genetic perturbations on metabolite production. Many methods consider growth coupling as the objective function, whereby it searches for mutants that maximise the growth and production rate. However, the final goal is to increase the production rate. Furthermore, they produce one single solution, though in reality, cells do not focus on one objective and they need to consider various different competing objectives. In this work, a method, termed ndsDSAFBA (non-dominated sorting Differential Search Algorithm and Flux Balance Analysis), has been developed to find the reaction knockouts involved in maximising the production rate and growth rate of the mutant, by incorporating Pareto dominance concepts. The proposed ndsDSAFBA method was validated using three genome-scale metabolic models. We obtained a set of non-dominated solutions, with each solution representing a different mutant strain. The results obtained were compared with the single objective optimisation (SOO) and multi-objective optimisation (MOO) methods. The results demonstrate that ndsDSAFBA is better than the other methods in terms of production rate and growth rate.
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spelling upm-225932020-02-06T03:20:49Z http://psasir.upm.edu.my/id/eprint/22593/ A non-dominated sorting Differential Search Algorithm Flux Balance Analysis (ndsDSAFBA) for in silico multiobjective optimization in identifying reactions knockout Mohd Daud, Kauthar Mohamad, Mohd Saberi Zakaria, Zalmiyah Hassan, Rohayanti Ali Shah, Zuraini Deris, Safa'ai Ibrahim, Zuwairie Napis, Suhaimi Sinnott, Richard O. Metabolic engineering is defined as improving the cellular activities of an organism by manipulating the metabolic, signal or regulatory network. In silico reaction knockout simulation is one of the techniques applied to analyse the effects of genetic perturbations on metabolite production. Many methods consider growth coupling as the objective function, whereby it searches for mutants that maximise the growth and production rate. However, the final goal is to increase the production rate. Furthermore, they produce one single solution, though in reality, cells do not focus on one objective and they need to consider various different competing objectives. In this work, a method, termed ndsDSAFBA (non-dominated sorting Differential Search Algorithm and Flux Balance Analysis), has been developed to find the reaction knockouts involved in maximising the production rate and growth rate of the mutant, by incorporating Pareto dominance concepts. The proposed ndsDSAFBA method was validated using three genome-scale metabolic models. We obtained a set of non-dominated solutions, with each solution representing a different mutant strain. The results obtained were compared with the single objective optimisation (SOO) and multi-objective optimisation (MOO) methods. The results demonstrate that ndsDSAFBA is better than the other methods in terms of production rate and growth rate. Elsevier 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/22593/1/A%20non-dominated%20sorting%20Differential%20Search%20Algorithm%20Flux%20Balance%20Analysis%20%28ndsDSAFBA%29%20for%20in%20silico%20multiobjective%20optimization%20in%20identifying%20reactions%20knockout.pdf Mohd Daud, Kauthar and Mohamad, Mohd Saberi and Zakaria, Zalmiyah and Hassan, Rohayanti and Ali Shah, Zuraini and Deris, Safa'ai and Ibrahim, Zuwairie and Napis, Suhaimi and Sinnott, Richard O. (2019) A non-dominated sorting Differential Search Algorithm Flux Balance Analysis (ndsDSAFBA) for in silico multiobjective optimization in identifying reactions knockout. Computers in Biology and Medicine, 113. art. no. 103390. pp. 1-13. ISSN 0010-4825; ESSN: 1879-0534 https://www.sciencedirect.com/science/article/pii/S0010482519302677?via%3Dihub#! 10.1016/j.compbiomed.2019.103390
spellingShingle Mohd Daud, Kauthar
Mohamad, Mohd Saberi
Zakaria, Zalmiyah
Hassan, Rohayanti
Ali Shah, Zuraini
Deris, Safa'ai
Ibrahim, Zuwairie
Napis, Suhaimi
Sinnott, Richard O.
A non-dominated sorting Differential Search Algorithm Flux Balance Analysis (ndsDSAFBA) for in silico multiobjective optimization in identifying reactions knockout
title A non-dominated sorting Differential Search Algorithm Flux Balance Analysis (ndsDSAFBA) for in silico multiobjective optimization in identifying reactions knockout
title_full A non-dominated sorting Differential Search Algorithm Flux Balance Analysis (ndsDSAFBA) for in silico multiobjective optimization in identifying reactions knockout
title_fullStr A non-dominated sorting Differential Search Algorithm Flux Balance Analysis (ndsDSAFBA) for in silico multiobjective optimization in identifying reactions knockout
title_full_unstemmed A non-dominated sorting Differential Search Algorithm Flux Balance Analysis (ndsDSAFBA) for in silico multiobjective optimization in identifying reactions knockout
title_short A non-dominated sorting Differential Search Algorithm Flux Balance Analysis (ndsDSAFBA) for in silico multiobjective optimization in identifying reactions knockout
title_sort non-dominated sorting differential search algorithm flux balance analysis (ndsdsafba) for in silico multiobjective optimization in identifying reactions knockout
url http://psasir.upm.edu.my/id/eprint/22593/
http://psasir.upm.edu.my/id/eprint/22593/
http://psasir.upm.edu.my/id/eprint/22593/
http://psasir.upm.edu.my/id/eprint/22593/1/A%20non-dominated%20sorting%20Differential%20Search%20Algorithm%20Flux%20Balance%20Analysis%20%28ndsDSAFBA%29%20for%20in%20silico%20multiobjective%20optimization%20in%20identifying%20reactions%20knockout.pdf