Synthesis of fatty esters by polyethylene glycol-modified lipase
Monomethoxypolyethylene glycols (PEG) of molecular masses 1900 and 5000 were activated using p-nitrophenyl chloroformate to form PEG–nitrophenyl carbonates (activated PEG) with high yield (96–98%). The activated PEG was covalently attached to Candida rugosa lipase. Increasing the molar ratio of acti...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Society of Chemical Industry
1995
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22572/ http://psasir.upm.edu.my/id/eprint/22572/1/Synthesis%20of%20fatty%20esters%20by%20polyethylene%20glycol-modified%20lipase.pdf |
| _version_ | 1848844521169747968 |
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| author | Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya Salleh, Abu Bakar |
| author_facet | Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya Salleh, Abu Bakar |
| author_sort | Basri, Mahiran |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Monomethoxypolyethylene glycols (PEG) of molecular masses 1900 and 5000 were activated using p-nitrophenyl chloroformate to form PEG–nitrophenyl carbonates (activated PEG) with high yield (96–98%). The activated PEG was covalently attached to Candida rugosa lipase. Increasing the molar ratio of activated PEG to the enzyme increased the degree of lipase modification. These modified lipases exhibited specific ester synthesis activities on organic solvents compared with native lipase. The degree of activity enhancement depended on the size of activated PEG used and the degree of modification of the enzyme. Maximal activity was attained after exhaustive of modification. The effects of different solvents, reaction temperature, and fatty acids on the esterification activity and the stability of the modified enzyme were investigated. The optimum esterification temperature (40°C) and preference of fatty acids as acyl donors of the modified lipase were very similar to those of the native enzyme. The modified lipase exhibited higher activity non-polar solvents than in polar solvents, and showed higher temperature, solvent and storage stability then the native lipase. |
| first_indexed | 2025-11-15T08:32:14Z |
| format | Article |
| id | upm-22572 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:32:14Z |
| publishDate | 1995 |
| publisher | Society of Chemical Industry |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-225722016-02-02T04:04:07Z http://psasir.upm.edu.my/id/eprint/22572/ Synthesis of fatty esters by polyethylene glycol-modified lipase Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya Salleh, Abu Bakar Monomethoxypolyethylene glycols (PEG) of molecular masses 1900 and 5000 were activated using p-nitrophenyl chloroformate to form PEG–nitrophenyl carbonates (activated PEG) with high yield (96–98%). The activated PEG was covalently attached to Candida rugosa lipase. Increasing the molar ratio of activated PEG to the enzyme increased the degree of lipase modification. These modified lipases exhibited specific ester synthesis activities on organic solvents compared with native lipase. The degree of activity enhancement depended on the size of activated PEG used and the degree of modification of the enzyme. Maximal activity was attained after exhaustive of modification. The effects of different solvents, reaction temperature, and fatty acids on the esterification activity and the stability of the modified enzyme were investigated. The optimum esterification temperature (40°C) and preference of fatty acids as acyl donors of the modified lipase were very similar to those of the native enzyme. The modified lipase exhibited higher activity non-polar solvents than in polar solvents, and showed higher temperature, solvent and storage stability then the native lipase. Society of Chemical Industry 1995-09 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22572/1/Synthesis%20of%20fatty%20esters%20by%20polyethylene%20glycol-modified%20lipase.pdf Basri, Mahiran and Ampon, Kamaruzaman and Wan Yunus, Wan Md. Zin and Abdul Razak, Che Nyonya and Salleh, Abu Bakar (1995) Synthesis of fatty esters by polyethylene glycol-modified lipase. Journal of Chemical Technology and Biotechnology, 64 (1). pp. 10-16. ISSN 0268-2575; ESSN: 1097-4660 http://onlinelibrary.wiley.com/doi/10.1002/jctb.280640103/abstract 10.1002/jctb.280640103 |
| spellingShingle | Basri, Mahiran Ampon, Kamaruzaman Wan Yunus, Wan Md. Zin Abdul Razak, Che Nyonya Salleh, Abu Bakar Synthesis of fatty esters by polyethylene glycol-modified lipase |
| title | Synthesis of fatty esters by polyethylene glycol-modified lipase |
| title_full | Synthesis of fatty esters by polyethylene glycol-modified lipase |
| title_fullStr | Synthesis of fatty esters by polyethylene glycol-modified lipase |
| title_full_unstemmed | Synthesis of fatty esters by polyethylene glycol-modified lipase |
| title_short | Synthesis of fatty esters by polyethylene glycol-modified lipase |
| title_sort | synthesis of fatty esters by polyethylene glycol-modified lipase |
| url | http://psasir.upm.edu.my/id/eprint/22572/ http://psasir.upm.edu.my/id/eprint/22572/ http://psasir.upm.edu.my/id/eprint/22572/ http://psasir.upm.edu.my/id/eprint/22572/1/Synthesis%20of%20fatty%20esters%20by%20polyethylene%20glycol-modified%20lipase.pdf |