Synthesis of medium-chain glycerides using lipase from Candida rugosa
Enzymatic synthesis of medium-chain glycerides (MCG) from capric acid and glycerol was studied using lipase from Candida rugosa. The effects of various reaction parameters such as time, molar ratio of substrates (mmol capric acid/mmol glycerol), amount of lipase, type of organic solvents, and initia...
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| Format: | Article |
| Language: | English English |
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AOCS Press
2000
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22570/ http://psasir.upm.edu.my/id/eprint/22570/1/Synthesis%20of%20medium-chain%20glycerides%20using%20lipase%20from%20Candida%20rugosa.pdf http://psasir.upm.edu.my/id/eprint/22570/7/J%20Americ%20Oil%20Chem%20Soc%20-%202000%20-%20Wong%20-%20Synthesis%20of%20medium%E2%80%90chain%20glycerides%20using%20lipase%20from%20Candida%20rugosa.pdf |
| _version_ | 1848844520623439872 |
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| author | Wong, W. C. Basri, Mahiran Abdul Razak, Che Nyonya Salleh, Abu Bakar |
| author_facet | Wong, W. C. Basri, Mahiran Abdul Razak, Che Nyonya Salleh, Abu Bakar |
| author_sort | Wong, W. C. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Enzymatic synthesis of medium-chain glycerides (MCG) from capric acid and glycerol was studied using lipase from Candida rugosa. The effects of various reaction parameters such as time, molar ratio of substrates (mmol capric acid/mmol glycerol), amount of lipase, type of organic solvents, and initial water activity (a w ) were studied. The best conditions tested for MCG synthesis at 37°C were, respectively, time, 24 h; molar ratio of substrates, 2.5; and amount of lipase, 100.0 mg. The use of organic solvents greatly influenced the activity of lipase in the synthesis of MCG. Generally, activity of lipase was high in nonpolar solvents with log P values from 3.50 to 4.50, where P is the partition coefficient between water and 1-octanol. The enzymatic synthesis of MCG was preferably carried out at an initial a w of 0.328, which resulted in maximal yield. Analysis of the products of reaction using gas chromatography showed that lipase from Candida rugosa seemed to produce more dicaprin and tricaprin than monocaprin. |
| first_indexed | 2025-11-15T08:32:14Z |
| format | Article |
| id | upm-22570 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:32:14Z |
| publishDate | 2000 |
| publisher | AOCS Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-225702024-08-05T07:58:40Z http://psasir.upm.edu.my/id/eprint/22570/ Synthesis of medium-chain glycerides using lipase from Candida rugosa Wong, W. C. Basri, Mahiran Abdul Razak, Che Nyonya Salleh, Abu Bakar Enzymatic synthesis of medium-chain glycerides (MCG) from capric acid and glycerol was studied using lipase from Candida rugosa. The effects of various reaction parameters such as time, molar ratio of substrates (mmol capric acid/mmol glycerol), amount of lipase, type of organic solvents, and initial water activity (a w ) were studied. The best conditions tested for MCG synthesis at 37°C were, respectively, time, 24 h; molar ratio of substrates, 2.5; and amount of lipase, 100.0 mg. The use of organic solvents greatly influenced the activity of lipase in the synthesis of MCG. Generally, activity of lipase was high in nonpolar solvents with log P values from 3.50 to 4.50, where P is the partition coefficient between water and 1-octanol. The enzymatic synthesis of MCG was preferably carried out at an initial a w of 0.328, which resulted in maximal yield. Analysis of the products of reaction using gas chromatography showed that lipase from Candida rugosa seemed to produce more dicaprin and tricaprin than monocaprin. AOCS Press 2000 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/22570/1/Synthesis%20of%20medium-chain%20glycerides%20using%20lipase%20from%20Candida%20rugosa.pdf text en http://psasir.upm.edu.my/id/eprint/22570/7/J%20Americ%20Oil%20Chem%20Soc%20-%202000%20-%20Wong%20-%20Synthesis%20of%20medium%E2%80%90chain%20glycerides%20using%20lipase%20from%20Candida%20rugosa.pdf Wong, W. C. and Basri, Mahiran and Abdul Razak, Che Nyonya and Salleh, Abu Bakar (2000) Synthesis of medium-chain glycerides using lipase from Candida rugosa. Journal of the American Oil Chemists' Society, 77 (1). pp. 85-88. ISSN 0003-021X; ESSN: 1558-9331 http://link.springer.com/article/10.1007%2Fs11746-000-0013-9 10.1007/s11746-000-0013-9 |
| spellingShingle | Wong, W. C. Basri, Mahiran Abdul Razak, Che Nyonya Salleh, Abu Bakar Synthesis of medium-chain glycerides using lipase from Candida rugosa |
| title | Synthesis of medium-chain glycerides using lipase from Candida rugosa |
| title_full | Synthesis of medium-chain glycerides using lipase from Candida rugosa |
| title_fullStr | Synthesis of medium-chain glycerides using lipase from Candida rugosa |
| title_full_unstemmed | Synthesis of medium-chain glycerides using lipase from Candida rugosa |
| title_short | Synthesis of medium-chain glycerides using lipase from Candida rugosa |
| title_sort | synthesis of medium-chain glycerides using lipase from candida rugosa |
| url | http://psasir.upm.edu.my/id/eprint/22570/ http://psasir.upm.edu.my/id/eprint/22570/ http://psasir.upm.edu.my/id/eprint/22570/ http://psasir.upm.edu.my/id/eprint/22570/1/Synthesis%20of%20medium-chain%20glycerides%20using%20lipase%20from%20Candida%20rugosa.pdf http://psasir.upm.edu.my/id/eprint/22570/7/J%20Americ%20Oil%20Chem%20Soc%20-%202000%20-%20Wong%20-%20Synthesis%20of%20medium%E2%80%90chain%20glycerides%20using%20lipase%20from%20Candida%20rugosa.pdf |