Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent
The characteristics of the membrane-bound lipase from a thermophilic Rhizopus oryzae were studied. The pH and temperature optima for lipase activity were at 7.0 and 37°C, respectively. The enzyme was stable and acidic conditions, retaining more than 80% of its initial activity at pH 4.0 after 30 min...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
AOCS Press
1999
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/22569/ http://psasir.upm.edu.my/id/eprint/22569/1/Characterization%20of%20membrane-bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae%20isolated%20from%20palm%20oil%20mill%20effluent.pdf http://psasir.upm.edu.my/id/eprint/22569/7/J%20Americ%20Oil%20Chem%20Soc%20-%201999%20-%20Razak%20-%20Characterization%20of%20membrane%E2%80%90bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae.pdf |
| _version_ | 1848844520345567232 |
|---|---|
| author | Abdul Razak, Che Nyonya Musani, Rosiah Basri, Mahiran Salleh, Abu Bakar |
| author_facet | Abdul Razak, Che Nyonya Musani, Rosiah Basri, Mahiran Salleh, Abu Bakar |
| author_sort | Abdul Razak, Che Nyonya |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The characteristics of the membrane-bound lipase from a thermophilic Rhizopus oryzae were studied. The pH and temperature optima for lipase activity were at 7.0 and 37°C, respectively. The enzyme was stable and acidic conditions, retaining more than 80% of its initial activity at pH 4.0 after 30 min incubation. It was stable up to 50°C with 70% of initial activity retained after 3 h incubation. The enzyme is 1,3 specific and exhibits substrate preference. Monoacid triglyceride substrates were hydrolyzed better than methyl esters, polyoxysorbitan and sorbitan substrates. |
| first_indexed | 2025-11-15T08:32:14Z |
| format | Article |
| id | upm-22569 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:32:14Z |
| publishDate | 1999 |
| publisher | AOCS Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-225692024-08-06T00:08:08Z http://psasir.upm.edu.my/id/eprint/22569/ Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent Abdul Razak, Che Nyonya Musani, Rosiah Basri, Mahiran Salleh, Abu Bakar The characteristics of the membrane-bound lipase from a thermophilic Rhizopus oryzae were studied. The pH and temperature optima for lipase activity were at 7.0 and 37°C, respectively. The enzyme was stable and acidic conditions, retaining more than 80% of its initial activity at pH 4.0 after 30 min incubation. It was stable up to 50°C with 70% of initial activity retained after 3 h incubation. The enzyme is 1,3 specific and exhibits substrate preference. Monoacid triglyceride substrates were hydrolyzed better than methyl esters, polyoxysorbitan and sorbitan substrates. AOCS Press 1999 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/22569/1/Characterization%20of%20membrane-bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae%20isolated%20from%20palm%20oil%20mill%20effluent.pdf text en http://psasir.upm.edu.my/id/eprint/22569/7/J%20Americ%20Oil%20Chem%20Soc%20-%201999%20-%20Razak%20-%20Characterization%20of%20membrane%E2%80%90bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae.pdf Abdul Razak, Che Nyonya and Musani, Rosiah and Basri, Mahiran and Salleh, Abu Bakar (1999) Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent. Journal of the American Oil Chemists' Society, 76 (2). pp. 171-174. ISSN 0003-021X; ESSN: 1558-9331 http://link.springer.com/article/10.1007%2Fs11746-999-0214-0 10.1007/s11746-999-0214-0 |
| spellingShingle | Abdul Razak, Che Nyonya Musani, Rosiah Basri, Mahiran Salleh, Abu Bakar Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
| title | Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
| title_full | Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
| title_fullStr | Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
| title_full_unstemmed | Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
| title_short | Characterization of membrane-bound lipase from a thermophilic Rhizopus oryzae isolated from palm oil mill effluent |
| title_sort | characterization of membrane-bound lipase from a thermophilic rhizopus oryzae isolated from palm oil mill effluent |
| url | http://psasir.upm.edu.my/id/eprint/22569/ http://psasir.upm.edu.my/id/eprint/22569/ http://psasir.upm.edu.my/id/eprint/22569/ http://psasir.upm.edu.my/id/eprint/22569/1/Characterization%20of%20membrane-bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae%20isolated%20from%20palm%20oil%20mill%20effluent.pdf http://psasir.upm.edu.my/id/eprint/22569/7/J%20Americ%20Oil%20Chem%20Soc%20-%201999%20-%20Razak%20-%20Characterization%20of%20membrane%E2%80%90bound%20lipase%20from%20a%20thermophilic%20Rhizopus%20oryzae.pdf |