A plate assay for primary screening of lipase activity
A method for primary plate assay to determine lipase activity was developed. Tween 80 was used as the substrate with either Victoria Blue B, methyl red or rhodamine B as the indicator. Lipolytic activity was determined by the formation of the zone of intensification of the indicator colour after 24...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Elsevier
1989
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22566/ http://psasir.upm.edu.my/id/eprint/22566/1/A%20plate%20assay%20for%20primary%20screening%20of%20lipase%20activity.pdf http://psasir.upm.edu.my/id/eprint/22566/7/1-s2.0-0167701289900304-main.pdf |
| _version_ | 1848844519462666240 |
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| author | Abd Samad, Mohd Yusoff Abdul Razak, Che Nyonya Salleh, Abu Bakar Wan Yunus, Wan Md. Zin Ampon, Kamaruzaman Basri, Mahiran |
| author_facet | Abd Samad, Mohd Yusoff Abdul Razak, Che Nyonya Salleh, Abu Bakar Wan Yunus, Wan Md. Zin Ampon, Kamaruzaman Basri, Mahiran |
| author_sort | Abd Samad, Mohd Yusoff |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | A method for primary plate assay to determine lipase activity was developed. Tween 80 was used as the substrate with either Victoria Blue B, methyl red or rhodamine B as the indicator. Lipolytic activity was determined by the formation of the zone of intensification of the indicator colour after 24 h. Similar results were obtained using Tween 20 and 60 as substrates. Intensity of the colour is greater than that of the trioleindye system and clearer than the hydrolysis zone of tributyrin plate. Tests using a commercial enzyme preparation and growth media with lipolytic activity showed that the zone of intensification increased with increased lipolytic activity. A linear relationship can be seen when log enzyme concentration is plotted against the diameter of zone of intensification. Using this technique, primary screening of lipolytic microorganisms can be conducted using the formation of zones of intensification around the colonies and mycelia. |
| first_indexed | 2025-11-15T08:32:13Z |
| format | Article |
| id | upm-22566 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-15T08:32:13Z |
| publishDate | 1989 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-225662024-07-24T08:29:17Z http://psasir.upm.edu.my/id/eprint/22566/ A plate assay for primary screening of lipase activity Abd Samad, Mohd Yusoff Abdul Razak, Che Nyonya Salleh, Abu Bakar Wan Yunus, Wan Md. Zin Ampon, Kamaruzaman Basri, Mahiran A method for primary plate assay to determine lipase activity was developed. Tween 80 was used as the substrate with either Victoria Blue B, methyl red or rhodamine B as the indicator. Lipolytic activity was determined by the formation of the zone of intensification of the indicator colour after 24 h. Similar results were obtained using Tween 20 and 60 as substrates. Intensity of the colour is greater than that of the trioleindye system and clearer than the hydrolysis zone of tributyrin plate. Tests using a commercial enzyme preparation and growth media with lipolytic activity showed that the zone of intensification increased with increased lipolytic activity. A linear relationship can be seen when log enzyme concentration is plotted against the diameter of zone of intensification. Using this technique, primary screening of lipolytic microorganisms can be conducted using the formation of zones of intensification around the colonies and mycelia. Elsevier 1989-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/22566/1/A%20plate%20assay%20for%20primary%20screening%20of%20lipase%20activity.pdf text en http://psasir.upm.edu.my/id/eprint/22566/7/1-s2.0-0167701289900304-main.pdf Abd Samad, Mohd Yusoff and Abdul Razak, Che Nyonya and Salleh, Abu Bakar and Wan Yunus, Wan Md. Zin and Ampon, Kamaruzaman and Basri, Mahiran (1989) A plate assay for primary screening of lipase activity. Journal of Microbiological Methods, 9 (1). pp. 51-56. ISSN 0167-7012; ESSN: 1872-8359 http://www.sciencedirect.com/science/article/pii/0167701289900304 10.1016/0167-7012(89)90030-4 |
| spellingShingle | Abd Samad, Mohd Yusoff Abdul Razak, Che Nyonya Salleh, Abu Bakar Wan Yunus, Wan Md. Zin Ampon, Kamaruzaman Basri, Mahiran A plate assay for primary screening of lipase activity |
| title | A plate assay for primary screening of lipase activity |
| title_full | A plate assay for primary screening of lipase activity |
| title_fullStr | A plate assay for primary screening of lipase activity |
| title_full_unstemmed | A plate assay for primary screening of lipase activity |
| title_short | A plate assay for primary screening of lipase activity |
| title_sort | plate assay for primary screening of lipase activity |
| url | http://psasir.upm.edu.my/id/eprint/22566/ http://psasir.upm.edu.my/id/eprint/22566/ http://psasir.upm.edu.my/id/eprint/22566/ http://psasir.upm.edu.my/id/eprint/22566/1/A%20plate%20assay%20for%20primary%20screening%20of%20lipase%20activity.pdf http://psasir.upm.edu.my/id/eprint/22566/7/1-s2.0-0167701289900304-main.pdf |