Subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production

The present work aimed to investigate the pretreatment of oil palm mesocarp fiber (OPMF) in subcritical H2O-CO2 at a temperature range from 150–200 °C and 20–180 min with CO2 pressure from 3–5 MPa. The pretreated solids and liquids from this process were separated by filtration and characterized. Xy...

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Main Authors: Ahmad, Norlailiza, Zakaria @ Mamat, Mohd Rafein, Mohd Yusoff, Mohd Zulkhairi, Fujimoto, Shinji, Inoue, Hiroyuki, Ariffin, Hidayah, Hassan, Mohd Ali, Shirai, Yoshihoto
Format: Article
Language:English
Published: MDPI 2018
Online Access:http://psasir.upm.edu.my/id/eprint/22550/
http://psasir.upm.edu.my/id/eprint/22550/1/22550.pdf
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author Ahmad, Norlailiza
Zakaria @ Mamat, Mohd Rafein
Mohd Yusoff, Mohd Zulkhairi
Fujimoto, Shinji
Inoue, Hiroyuki
Ariffin, Hidayah
Hassan, Mohd Ali
Shirai, Yoshihoto
author_facet Ahmad, Norlailiza
Zakaria @ Mamat, Mohd Rafein
Mohd Yusoff, Mohd Zulkhairi
Fujimoto, Shinji
Inoue, Hiroyuki
Ariffin, Hidayah
Hassan, Mohd Ali
Shirai, Yoshihoto
author_sort Ahmad, Norlailiza
building UPM Institutional Repository
collection Online Access
description The present work aimed to investigate the pretreatment of oil palm mesocarp fiber (OPMF) in subcritical H2O-CO2 at a temperature range from 150–200 °C and 20–180 min with CO2 pressure from 3–5 MPa. The pretreated solids and liquids from this process were separated by filtration and characterized. Xylooligosaccharides (XOs), sugar monomers, acids, furans and phenols in the pretreated liquids were analyzed by using HPLC. XOs with a degree of polymerization X2–X4 comprising xylobiose, xylotriose, xylotetraose were analyzed by using HPAEC-PAD. Enzymatic hydrolysis was performed on cellulose-rich pretreated solids to observe xylose and glucose production. An optimal condition for XOs production was achieved at 180 °C, 60 min, 3 MPa and the highest XOs obtained was 81.60 mg/g which corresponded to 36.59% of XOs yield from total xylan of OPMF. The highest xylose and glucose yields obtained from pretreated solids were 29.96% and 84.65%, respectively at cellulase loading of 10 FPU/g-substrate.
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institution Universiti Putra Malaysia
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language English
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spelling upm-225502020-04-15T16:46:46Z http://psasir.upm.edu.my/id/eprint/22550/ Subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production Ahmad, Norlailiza Zakaria @ Mamat, Mohd Rafein Mohd Yusoff, Mohd Zulkhairi Fujimoto, Shinji Inoue, Hiroyuki Ariffin, Hidayah Hassan, Mohd Ali Shirai, Yoshihoto The present work aimed to investigate the pretreatment of oil palm mesocarp fiber (OPMF) in subcritical H2O-CO2 at a temperature range from 150–200 °C and 20–180 min with CO2 pressure from 3–5 MPa. The pretreated solids and liquids from this process were separated by filtration and characterized. Xylooligosaccharides (XOs), sugar monomers, acids, furans and phenols in the pretreated liquids were analyzed by using HPLC. XOs with a degree of polymerization X2–X4 comprising xylobiose, xylotriose, xylotetraose were analyzed by using HPAEC-PAD. Enzymatic hydrolysis was performed on cellulose-rich pretreated solids to observe xylose and glucose production. An optimal condition for XOs production was achieved at 180 °C, 60 min, 3 MPa and the highest XOs obtained was 81.60 mg/g which corresponded to 36.59% of XOs yield from total xylan of OPMF. The highest xylose and glucose yields obtained from pretreated solids were 29.96% and 84.65%, respectively at cellulase loading of 10 FPU/g-substrate. MDPI 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/22550/1/22550.pdf Ahmad, Norlailiza and Zakaria @ Mamat, Mohd Rafein and Mohd Yusoff, Mohd Zulkhairi and Fujimoto, Shinji and Inoue, Hiroyuki and Ariffin, Hidayah and Hassan, Mohd Ali and Shirai, Yoshihoto (2018) Subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production. Molecules, 23 (6). art. no. 1310. pp. 1-14. ISSN 1420-3049 https://www.mdpi.com/1420-3049/23/6/1310 10.3390/molecules23061310
spellingShingle Ahmad, Norlailiza
Zakaria @ Mamat, Mohd Rafein
Mohd Yusoff, Mohd Zulkhairi
Fujimoto, Shinji
Inoue, Hiroyuki
Ariffin, Hidayah
Hassan, Mohd Ali
Shirai, Yoshihoto
Subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production
title Subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production
title_full Subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production
title_fullStr Subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production
title_full_unstemmed Subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production
title_short Subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production
title_sort subcritical water-carbon dioxide pretreatment of oil palm mesocarp fiber for xylooligosaccharide and glucose production
url http://psasir.upm.edu.my/id/eprint/22550/
http://psasir.upm.edu.my/id/eprint/22550/
http://psasir.upm.edu.my/id/eprint/22550/
http://psasir.upm.edu.my/id/eprint/22550/1/22550.pdf