Sequential optimization of production of a thermostable and organic solvent tolerant lipase by recombinant Escherichia coli
Several medium formulations were screened for the production of a thermostable and organic solvent tolerant lipase by a recombinant Escherichia coli BL21. The highest lipase production (28.9 ± 4.1 IU/mL) was obtained in Luria Bertani medium with the addition of 1% (w/v) glucose. The medium formulati...
| Main Authors: | Nelofer, Rubina, Ramanan, Ramakrishnan Nagasundara, Raja Abdul Rahman, Raja Noor Zaliha, Basri, Mahiran, Ariff, Arbakariya |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2011
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/22410/ http://psasir.upm.edu.my/id/eprint/22410/1/Sequential%20optimization%20of%20production%20of%20a%20thermostable%20and%20organic%20solvent%20tolerant%20lipase%20by%20recombinant%20Escherichia%20coli.pdf |
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