Antioxidant activities of different parts of Gnetum gnemon L.

Analyses on biological activities of Gnetum gnemon were done to determine the total phenolic and antioxidants of the plant. Four parts of G. gnemon were used in this study, which were leaf, bark, twig, and seeds of the plant. All parts were extracted in methanol, ethanol, hexane, chloroform and hot...

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Main Authors: Wazir, Dayana, Ahmad, Syahida, Muse, Radzali, Mahmood, Maziah, Abd. Shukor, Mohd. Yunus
Format: Article
Published: Springer Verlag 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22400/
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author Wazir, Dayana
Ahmad, Syahida
Muse, Radzali
Mahmood, Maziah
Abd. Shukor, Mohd. Yunus
author_facet Wazir, Dayana
Ahmad, Syahida
Muse, Radzali
Mahmood, Maziah
Abd. Shukor, Mohd. Yunus
author_sort Wazir, Dayana
building UPM Institutional Repository
collection Online Access
description Analyses on biological activities of Gnetum gnemon were done to determine the total phenolic and antioxidants of the plant. Four parts of G. gnemon were used in this study, which were leaf, bark, twig, and seeds of the plant. All parts were extracted in methanol, ethanol, hexane, chloroform and hot water using reflux. The total phenolic content of the plant extracts were determined by using Folin-Ciocalteu method. The results demonstrated that the bark from hot water extract showed the highest total phenolic at 10.71 ± 0.01 mg GAE/ FDW, while the lowest was chloroform extract of seed at 2.15 ± 0.01 mg GAE/ FDW. The antioxidant activity of the plant extracts were determined by using DPPH and FRAP assays, respectively. The DPPH results showed that all plant extracts demonstrated weak free radical scavenging activity tested at the final concentration of 300 μg/ml. In contrast, the methanolic twig extract showed strong reducing power activity (FRAP) at 83.55 ± 1.05%, while the hot water seed extract showed the least activity at 41.86 ± 4.22% tested at the final concentration of 300 μg/ml. However, there were no correlation between total phenolics and both antioxidant assays tested.
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spelling upm-224002015-12-07T06:44:44Z http://psasir.upm.edu.my/id/eprint/22400/ Antioxidant activities of different parts of Gnetum gnemon L. Wazir, Dayana Ahmad, Syahida Muse, Radzali Mahmood, Maziah Abd. Shukor, Mohd. Yunus Analyses on biological activities of Gnetum gnemon were done to determine the total phenolic and antioxidants of the plant. Four parts of G. gnemon were used in this study, which were leaf, bark, twig, and seeds of the plant. All parts were extracted in methanol, ethanol, hexane, chloroform and hot water using reflux. The total phenolic content of the plant extracts were determined by using Folin-Ciocalteu method. The results demonstrated that the bark from hot water extract showed the highest total phenolic at 10.71 ± 0.01 mg GAE/ FDW, while the lowest was chloroform extract of seed at 2.15 ± 0.01 mg GAE/ FDW. The antioxidant activity of the plant extracts were determined by using DPPH and FRAP assays, respectively. The DPPH results showed that all plant extracts demonstrated weak free radical scavenging activity tested at the final concentration of 300 μg/ml. In contrast, the methanolic twig extract showed strong reducing power activity (FRAP) at 83.55 ± 1.05%, while the hot water seed extract showed the least activity at 41.86 ± 4.22% tested at the final concentration of 300 μg/ml. However, there were no correlation between total phenolics and both antioxidant assays tested. Springer Verlag 2011-07 Article PeerReviewed Wazir, Dayana and Ahmad, Syahida and Muse, Radzali and Mahmood, Maziah and Abd. Shukor, Mohd. Yunus (2011) Antioxidant activities of different parts of Gnetum gnemon L. Journal of Plant Biochemistry and Biotechnology, 20 (2). pp. 234-240. ISSN 0971-7811; ESSN: 0974-1275 http://link.springer.com/article/10.1007%2Fs13562-011-0051-8 10.1007/s13562-011-0051-8
spellingShingle Wazir, Dayana
Ahmad, Syahida
Muse, Radzali
Mahmood, Maziah
Abd. Shukor, Mohd. Yunus
Antioxidant activities of different parts of Gnetum gnemon L.
title Antioxidant activities of different parts of Gnetum gnemon L.
title_full Antioxidant activities of different parts of Gnetum gnemon L.
title_fullStr Antioxidant activities of different parts of Gnetum gnemon L.
title_full_unstemmed Antioxidant activities of different parts of Gnetum gnemon L.
title_short Antioxidant activities of different parts of Gnetum gnemon L.
title_sort antioxidant activities of different parts of gnetum gnemon l.
url http://psasir.upm.edu.my/id/eprint/22400/
http://psasir.upm.edu.my/id/eprint/22400/
http://psasir.upm.edu.my/id/eprint/22400/