Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein
Dendrobium Sonia 17 calluses were used for co-transformation study using particle bombardment. The bombarded transformed callus tissues were selected using half-strength MS medium containing 25 mg dm−3 hygromycin. Expression of green fluorescent protein (GFP) was observed in the callus and protocorm...
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| Format: | Article |
| Language: | English |
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Springer
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22384/ http://psasir.upm.edu.my/id/eprint/22384/1/Selection%20of%20co-transformed%20Dendrobium%20Sonia%2017%20using%20hygromycin%20and%20green%20fluorescent%20protein.pdf |
| _version_ | 1848844470600073216 |
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| author | Tee, Chong Siang Mahmood, Maziah Tan, Chon Seng Abdullah, Mohd Puad |
| author_facet | Tee, Chong Siang Mahmood, Maziah Tan, Chon Seng Abdullah, Mohd Puad |
| author_sort | Tee, Chong Siang |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Dendrobium Sonia 17 calluses were used for co-transformation study using particle bombardment. The bombarded transformed callus tissues were selected using half-strength MS medium containing 25 mg dm−3 hygromycin. Expression of green fluorescent protein (GFP) was observed in the callus and protocorm-like body (PLBs) tissues survived on the selection medium. The presence of green fluorescence protein (sgfp), hygromycin-B-phosphotransferase (hptII) and β-glucuronidase (uidA) genes in the transformed tissues were verified using PCR, Southern blot and dot blot analyses. Based on the results from PCR and expression of sgfp and uidA genes in the calluses and PLBs survived from hygromycin selection, we reported the co-transformation of sgfp, hptII and uidA genes into Dendrobium Sonia 17. GFP-expressing tissues were also observed in the regenerated transformed plantlets. |
| first_indexed | 2025-11-15T08:31:26Z |
| format | Article |
| id | upm-22384 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:31:26Z |
| publishDate | 2011 |
| publisher | Springer |
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| spelling | upm-223842016-01-12T04:50:43Z http://psasir.upm.edu.my/id/eprint/22384/ Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein Tee, Chong Siang Mahmood, Maziah Tan, Chon Seng Abdullah, Mohd Puad Dendrobium Sonia 17 calluses were used for co-transformation study using particle bombardment. The bombarded transformed callus tissues were selected using half-strength MS medium containing 25 mg dm−3 hygromycin. Expression of green fluorescent protein (GFP) was observed in the callus and protocorm-like body (PLBs) tissues survived on the selection medium. The presence of green fluorescence protein (sgfp), hygromycin-B-phosphotransferase (hptII) and β-glucuronidase (uidA) genes in the transformed tissues were verified using PCR, Southern blot and dot blot analyses. Based on the results from PCR and expression of sgfp and uidA genes in the calluses and PLBs survived from hygromycin selection, we reported the co-transformation of sgfp, hptII and uidA genes into Dendrobium Sonia 17. GFP-expressing tissues were also observed in the regenerated transformed plantlets. Springer 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22384/1/Selection%20of%20co-transformed%20Dendrobium%20Sonia%2017%20using%20hygromycin%20and%20green%20fluorescent%20protein.pdf Tee, Chong Siang and Mahmood, Maziah and Tan, Chon Seng and Abdullah, Mohd Puad (2011) Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein. Biologia Plantarum, 55 (3). pp. 572-576. ISSN 0006-3134; ESSN: 1573-8264 http://link.springer.com/article/10.1007%2Fs10535-011-0128-1 10.1007/s10535-011-0128-1 |
| spellingShingle | Tee, Chong Siang Mahmood, Maziah Tan, Chon Seng Abdullah, Mohd Puad Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein |
| title | Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein |
| title_full | Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein |
| title_fullStr | Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein |
| title_full_unstemmed | Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein |
| title_short | Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein |
| title_sort | selection of co-transformed dendrobium sonia 17 using hygromycin and green fluorescent protein |
| url | http://psasir.upm.edu.my/id/eprint/22384/ http://psasir.upm.edu.my/id/eprint/22384/ http://psasir.upm.edu.my/id/eprint/22384/ http://psasir.upm.edu.my/id/eprint/22384/1/Selection%20of%20co-transformed%20Dendrobium%20Sonia%2017%20using%20hygromycin%20and%20green%20fluorescent%20protein.pdf |