Porcine-specific polymerase chain reaction assay based on mitochondrial D-loop gene for identification of pork in raw meat
The porcine-specific polymerase chain reaction assay was developed to detect the presence of pork in raw meat either in pure or in a mixture with different meat species. A porcine-specific DNA fragment could be observed by using a porcine-specific primer designed based on a porcine specific sequence...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22382/ http://psasir.upm.edu.my/id/eprint/22382/1/Porcine.pdf |
| _version_ | 1848844470027550720 |
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| author | Che Man, Yaakob Mustafa, Shuhaimi Khairil Mokhtar, Nur Fadhilah Nordin, Rumaisa Sazili, Awis Qurni |
| author_facet | Che Man, Yaakob Mustafa, Shuhaimi Khairil Mokhtar, Nur Fadhilah Nordin, Rumaisa Sazili, Awis Qurni |
| author_sort | Che Man, Yaakob |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The porcine-specific polymerase chain reaction assay was developed to detect the presence of pork in raw meat either in pure or in a mixture with different meat species. A porcine-specific DNA fragment could be observed by using a porcine-specific primer designed based on a porcine specific sequence of mitochondrial D-loop gene. Amplification of porcine DNA produced 174 bp porcine-specific DNA fragment and no polymerase chain reaction products were detected when assay applied to DNA of other meat species confirming the specificity of the primers. The assay was able to detect as low as 0.1% (v/v) porcine DNA spiked on DNA of cattle, sheep, goat, chicken, and deer. Furthermore, a detection limit of 0.001 ng/μL porcine DNA showed the high sensitivity conferred by the developed porcine-specific polymerase chain reaction assay. |
| first_indexed | 2025-11-15T08:31:26Z |
| format | Article |
| id | upm-22382 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:31:26Z |
| publishDate | 2012 |
| publisher | Taylor & Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-223822015-10-02T03:18:20Z http://psasir.upm.edu.my/id/eprint/22382/ Porcine-specific polymerase chain reaction assay based on mitochondrial D-loop gene for identification of pork in raw meat Che Man, Yaakob Mustafa, Shuhaimi Khairil Mokhtar, Nur Fadhilah Nordin, Rumaisa Sazili, Awis Qurni The porcine-specific polymerase chain reaction assay was developed to detect the presence of pork in raw meat either in pure or in a mixture with different meat species. A porcine-specific DNA fragment could be observed by using a porcine-specific primer designed based on a porcine specific sequence of mitochondrial D-loop gene. Amplification of porcine DNA produced 174 bp porcine-specific DNA fragment and no polymerase chain reaction products were detected when assay applied to DNA of other meat species confirming the specificity of the primers. The assay was able to detect as low as 0.1% (v/v) porcine DNA spiked on DNA of cattle, sheep, goat, chicken, and deer. Furthermore, a detection limit of 0.001 ng/μL porcine DNA showed the high sensitivity conferred by the developed porcine-specific polymerase chain reaction assay. Taylor & Francis 2012 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22382/1/Porcine.pdf Che Man, Yaakob and Mustafa, Shuhaimi and Khairil Mokhtar, Nur Fadhilah and Nordin, Rumaisa and Sazili, Awis Qurni (2012) Porcine-specific polymerase chain reaction assay based on mitochondrial D-loop gene for identification of pork in raw meat. International Journal of Food Properties, 15 (1). pp. 134-144. ISSN 1094-2912; ESSN: 1532-2386 http://www.tandfonline.com/doi/abs/10.1080/10942911003754692 10.1080/10942911003754692 |
| spellingShingle | Che Man, Yaakob Mustafa, Shuhaimi Khairil Mokhtar, Nur Fadhilah Nordin, Rumaisa Sazili, Awis Qurni Porcine-specific polymerase chain reaction assay based on mitochondrial D-loop gene for identification of pork in raw meat |
| title | Porcine-specific polymerase chain reaction assay based on mitochondrial D-loop gene for identification of pork in raw meat |
| title_full | Porcine-specific polymerase chain reaction assay based on mitochondrial D-loop gene for identification of pork in raw meat |
| title_fullStr | Porcine-specific polymerase chain reaction assay based on mitochondrial D-loop gene for identification of pork in raw meat |
| title_full_unstemmed | Porcine-specific polymerase chain reaction assay based on mitochondrial D-loop gene for identification of pork in raw meat |
| title_short | Porcine-specific polymerase chain reaction assay based on mitochondrial D-loop gene for identification of pork in raw meat |
| title_sort | porcine-specific polymerase chain reaction assay based on mitochondrial d-loop gene for identification of pork in raw meat |
| url | http://psasir.upm.edu.my/id/eprint/22382/ http://psasir.upm.edu.my/id/eprint/22382/ http://psasir.upm.edu.my/id/eprint/22382/ http://psasir.upm.edu.my/id/eprint/22382/1/Porcine.pdf |