An efficient protocol for particle bombardment-mediated transformation of Centella asiatica callus
In this study, we report an optimization of particle bombardment transformation system for Centella asiatica callus. A total of eight parameters affecting the genetic transformation system were optimized using the synthetic green fluorescent protein (sGFP) as a reporter driven by the CaMV 35S promot...
| Main Authors: | Lai, Kok Song, Abdullah, Puad, Yusoff, Khatijah, Mahmood, Maziah |
|---|---|
| Format: | Article |
| Published: |
Springer-Verlag
2011
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/22377/ |
Similar Items
Paraquat (methyl viologen) toxicity in centella asiatica callus cultures
by: Mohd Fadzillah, Nor'Aini, et al.
Published: (2006)
by: Mohd Fadzillah, Nor'Aini, et al.
Published: (2006)
Extracellular matrix as the early structural marker for Centella asiatica embryogenic tissues
by: Lai, Kok Song, et al.
Published: (2011)
by: Lai, Kok Song, et al.
Published: (2011)
Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina
by: Chew, Yee-Chern, et al.
Published: (2019)
by: Chew, Yee-Chern, et al.
Published: (2019)
Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina
by: Chew, Yee Chern, et al.
Published: (2019)
by: Chew, Yee Chern, et al.
Published: (2019)
Expression of Haemagglutinin-Neuraminidase Envelope Protein of Newcastle Disease Virus Strain Af2240 in Centella Asiatica (Pegaga) Embryogenic Calli Through Optimized Particle Bombardment Method
by: Lai, Kok Song
Published: (2008)
by: Lai, Kok Song
Published: (2008)
Morphological and physiological characterization of embryogenic and non-embryogenic tissues of Centella asiatica
by: Lai, Kok Song, et al.
Published: (2014)
by: Lai, Kok Song, et al.
Published: (2014)
Callus formation, somatic embryogenesis and development of transformation protocol by practicle bombardment in plantain banana
by: Deswina, Puspita
Published: (2001)
by: Deswina, Puspita
Published: (2001)
a-Tocopherol, ascorbic acid and carotenoid content in Centella asiatica leaf tissue and callus cultures.
by: Kusnan, Misri, et al.
Published: (2011)
by: Kusnan, Misri, et al.
Published: (2011)
Transient Transformation of Oncidium Taka by Particle Bombardment
by: Wee, Chien Yeong
Published: (2006)
by: Wee, Chien Yeong
Published: (2006)
Optimization of biolistic bombardment parameters for Dendrobium sonia 17 calluses using GFP and GUS as the reporter system
by: Tee, Chong Siang, et al.
Published: (2005)
by: Tee, Chong Siang, et al.
Published: (2005)
Comparison of particle bombardment-transformation parameters between stem and calli explants of Aglaonema simplex
by: Ma, Nyuk Ling, et al.
Published: (2013)
by: Ma, Nyuk Ling, et al.
Published: (2013)
Barium levels in soils and Centella asiatica
by: Ong, Ghim Hock, et al.
Published: (2013)
by: Ong, Ghim Hock, et al.
Published: (2013)
Triterpene Production in Centella Asiatica (L.) Urban (Pegaga) Callus and Cell Suspension Cultures
by: Ling, Anna Pick Kiong
Published: (2004)
by: Ling, Anna Pick Kiong
Published: (2004)
Genetic Transformation of Metroxylon sagu (Rottb.) Cultures via Agrobacterium-Mediated and Particle Bombardment
by: Evra Raunie, Ibrahim, et al.
Published: (2014)
by: Evra Raunie, Ibrahim, et al.
Published: (2014)
Effect of plant growth regulators on callus, cell suspension and cell line selection for flavonoid production from pegaga (centella asiatica L. urban)
by: Tan, Suat Hian, et al.
Published: (2010)
by: Tan, Suat Hian, et al.
Published: (2010)
Optimization of particle bombardment parameters for DNA delivery into the male flowers of banana
by: Mahdavi, Fatemeh, et al.
Published: (2014)
by: Mahdavi, Fatemeh, et al.
Published: (2014)
Pegaga (Centella asiatica)
Published: (2016)
Published: (2016)
Genetic Transformation of Rice Cultivars Using Particle Bombardment for Salinity Tolerance
by: Htwe, Nwe Nwe
Published: (2011)
by: Htwe, Nwe Nwe
Published: (2011)
Effects of particle size, method and duration of extraction on asiaticoside in Centella asiatica
by: Darnis, Deny Susanti, et al.
Published: (2016)
by: Darnis, Deny Susanti, et al.
Published: (2016)
Physical and biological parameters affecting transient GUS and GFP expression in banana via particle bombardment
by: Subramaniam, Sreeramanan, et al.
Published: (2005)
by: Subramaniam, Sreeramanan, et al.
Published: (2005)
Centella asiatica: a review
by: Ahmed, Afnan Sh., et al.
Published: (2016)
by: Ahmed, Afnan Sh., et al.
Published: (2016)
Centella asiatica: a potential candidate to assess the uranium contamination in soil
by: Yap, Chee Kong, et al.
Published: (2013)
by: Yap, Chee Kong, et al.
Published: (2013)
Accumulation of heavy metals and antioxidative enzymes of Centella asiatica in relation to metals of the soils
by: Ong, Ghim Hock, et al.
Published: (2013)
by: Ong, Ghim Hock, et al.
Published: (2013)
Accumulation of heavy metals and antioxidative enzymes of
Centella asiatica in relation to metals of the soils.
by: Ong, G. H., et al.
Published: (2013)
by: Ong, G. H., et al.
Published: (2013)
Synergistic and antagonistic effects of zinc bioaccumulation with lead and antioxidant activities in Centella asiatica
by: Ong, Ghim Hock, et al.
Published: (2013)
by: Ong, Ghim Hock, et al.
Published: (2013)
Kinetics and modelling of cell growth and substrate uptake in Centella asiatica cell culture
by: Omar, Rozita, et al.
Published: (2006)
by: Omar, Rozita, et al.
Published: (2006)
Metal chelating activity of Centella asiatica
by: Mohd Salim, Roshan Jahn, et al.
Published: (2010)
by: Mohd Salim, Roshan Jahn, et al.
Published: (2010)
Triterpene composition and bioactivities of Centella asiatica
by: Hashim, Puziah, et al.
Published: (2011)
by: Hashim, Puziah, et al.
Published: (2011)
Anti-inflammatory activity
of centella asiatica
by: Isuparan, Dinishkumari
Published: (2012)
by: Isuparan, Dinishkumari
Published: (2012)
An investigation of arsenic contamination in Peninsular Malaysia based on Centella asiatica and soil samples
by: Ong, Ghim Hock, et al.
Published: (2013)
by: Ong, Ghim Hock, et al.
Published: (2013)
Optimization of an efficient semi-solid culture protocol for sterilization and plant regeneration of Centella asiatica (L.) as a medicinal herb.
by: Moghaddam, Sina Siavash, et al.
Published: (2011)
by: Moghaddam, Sina Siavash, et al.
Published: (2011)
Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles
by: Latif, Muhammad Sohail
Published: (2019)
by: Latif, Muhammad Sohail
Published: (2019)
The effect of Cu exposure on the bioaccumulation of Zn and antioxidant activities in different parts of Centella asiatica.
by: Ong, G.H., et al.
Published: (2011)
by: Ong, G.H., et al.
Published: (2011)
Heavy metal accumulation in a medicinal plant Centella asiatica from Peninsular Malaysia
by: Ong, Hock Ghim, et al.
Published: (2011)
by: Ong, Hock Ghim, et al.
Published: (2011)
Optimisation of Genetic Transformation Parameters Through Microprojectile Bombardment Using Green Fluorescent Protein Reporter System in Dendrobium ‘Sonia 17’ Callus
by: Tee, Chong Siang
Published: (2004)
by: Tee, Chong Siang
Published: (2004)
Optimisation of transformation system for chilli embryo (Capsicum annuum variety Kulai) using particle bombardment
by: Zainuddin, Zarina, et al.
Published: (2011)
by: Zainuddin, Zarina, et al.
Published: (2011)
Antioxidative Constituents of Centella Asiatica L. Urban
by: Yusuf, Norhayati
Published: (2011)
by: Yusuf, Norhayati
Published: (2011)
Profiling of Centella asiatica (L.) urban extract
by: Zainol, N. A., et al.
Published: (2008)
by: Zainol, N. A., et al.
Published: (2008)
Synergistic and antagonistic effects of Zinc Bioaccumulation with lead and antioxidant activities in centella asiatica
by: G.H., Ong, et al.
Published: (2013)
by: G.H., Ong, et al.
Published: (2013)
Magnesium in local edible ulam (Centella asiatica) and its relation to their habitat soils in Peninsular Malaysia
by: Ong, Ghim Hock, et al.
Published: (2017)
by: Ong, Ghim Hock, et al.
Published: (2017)
Similar Items
-
Paraquat (methyl viologen) toxicity in centella asiatica callus cultures
by: Mohd Fadzillah, Nor'Aini, et al.
Published: (2006) -
Extracellular matrix as the early structural marker for Centella asiatica embryogenic tissues
by: Lai, Kok Song, et al.
Published: (2011) -
Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina
by: Chew, Yee-Chern, et al.
Published: (2019) -
Development of an efficient particle bombardment transformation system for the endemic orchid, Phalaenopsis bellina
by: Chew, Yee Chern, et al.
Published: (2019) -
Expression of Haemagglutinin-Neuraminidase Envelope Protein of Newcastle Disease Virus Strain Af2240 in Centella Asiatica (Pegaga) Embryogenic Calli Through Optimized Particle Bombardment Method
by: Lai, Kok Song
Published: (2008)