Flavonoids production in Hydrocotyle bonariensis callus tissues
Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensistreated with 2 mg/L 2,4-D and 1 mg/L kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). The data obtained showed that the best medium for the highest flavonoids production...
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22376/ http://psasir.upm.edu.my/id/eprint/22376/1/Flavonoids%20production%20in%20Hydrocotyle%20bonariensis%20callus%20tissues.pdf |
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| author | Masoumian, M. Ariff, Arbakariya Ahmad, Syahida Mahmood, Maziah |
| author_facet | Masoumian, M. Ariff, Arbakariya Ahmad, Syahida Mahmood, Maziah |
| author_sort | Masoumian, M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensistreated with 2 mg/L 2,4-D and 1 mg/L kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). The data obtained showed that the best medium for the highest flavonoids production was in DKW basal medium. Fructose as a carbon source increased the flavonoids content by 3%. Callus treated with 2 mg/L 2,4-D in comparison with the other auxins and among the cytokinins, kinetin and thidiazuron (TDZ) at 1 mg/L enhanced the flavonoid accumulation. Higher flavonoid production was obtained in combination with the 2 mg/L 2,4-D and 1 mg/L kinetin, while the pH value which was higher than 5.7 showed inhibitory effect on flavonoids production. It was found that the highest flavonoids were produced at 16 days of culture. |
| first_indexed | 2025-11-15T08:31:24Z |
| format | Article |
| id | upm-22376 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:31:24Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| recordtype | eprints |
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| spelling | upm-223762015-10-16T07:16:22Z http://psasir.upm.edu.my/id/eprint/22376/ Flavonoids production in Hydrocotyle bonariensis callus tissues Masoumian, M. Ariff, Arbakariya Ahmad, Syahida Mahmood, Maziah Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensistreated with 2 mg/L 2,4-D and 1 mg/L kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). The data obtained showed that the best medium for the highest flavonoids production was in DKW basal medium. Fructose as a carbon source increased the flavonoids content by 3%. Callus treated with 2 mg/L 2,4-D in comparison with the other auxins and among the cytokinins, kinetin and thidiazuron (TDZ) at 1 mg/L enhanced the flavonoid accumulation. Higher flavonoid production was obtained in combination with the 2 mg/L 2,4-D and 1 mg/L kinetin, while the pH value which was higher than 5.7 showed inhibitory effect on flavonoids production. It was found that the highest flavonoids were produced at 16 days of culture. Academic Journals 2011-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22376/1/Flavonoids%20production%20in%20Hydrocotyle%20bonariensis%20callus%20tissues.pdf Masoumian, M. and Ariff, Arbakariya and Ahmad, Syahida and Mahmood, Maziah (2011) Flavonoids production in Hydrocotyle bonariensis callus tissues. Journal of Medicinal Plants Research, 5 (9). pp. 1564-1574. ISSN 1996-0875 http://www.academicjournals.org/journal/JMPR/article-abstract/44D150E17762 |
| spellingShingle | Masoumian, M. Ariff, Arbakariya Ahmad, Syahida Mahmood, Maziah Flavonoids production in Hydrocotyle bonariensis callus tissues |
| title | Flavonoids production in Hydrocotyle bonariensis callus tissues |
| title_full | Flavonoids production in Hydrocotyle bonariensis callus tissues |
| title_fullStr | Flavonoids production in Hydrocotyle bonariensis callus tissues |
| title_full_unstemmed | Flavonoids production in Hydrocotyle bonariensis callus tissues |
| title_short | Flavonoids production in Hydrocotyle bonariensis callus tissues |
| title_sort | flavonoids production in hydrocotyle bonariensis callus tissues |
| url | http://psasir.upm.edu.my/id/eprint/22376/ http://psasir.upm.edu.my/id/eprint/22376/ http://psasir.upm.edu.my/id/eprint/22376/1/Flavonoids%20production%20in%20Hydrocotyle%20bonariensis%20callus%20tissues.pdf |