Flavonoids production in Hydrocotyle bonariensis callus tissues
Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensistreated with 2 mg/L 2,4-D and 1 mg/L kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). The data obtained showed that the best medium for the highest flavonoids production...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22376/ http://psasir.upm.edu.my/id/eprint/22376/1/Flavonoids%20production%20in%20Hydrocotyle%20bonariensis%20callus%20tissues.pdf |
| Summary: | Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensistreated with 2 mg/L 2,4-D and 1 mg/L kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). The data obtained showed that the best medium for the highest flavonoids production was in DKW basal medium. Fructose as a carbon source increased the flavonoids content by 3%. Callus treated with 2 mg/L 2,4-D in comparison with the other auxins and among the cytokinins, kinetin and thidiazuron (TDZ) at 1 mg/L enhanced the flavonoid accumulation. Higher flavonoid production was obtained in combination with the 2 mg/L 2,4-D and 1 mg/L kinetin, while the pH value which was higher than 5.7 showed inhibitory effect on flavonoids production. It was found that the highest flavonoids were produced at 16 days of culture. |
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