Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues
Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensis treated with 2 mg/l of 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). To optimize the precursors to increase the prod...
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| Language: | English |
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Academic Journals
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22375/ http://psasir.upm.edu.my/id/eprint/22375/1/Effect%20of%20precursors%20on%20flavonoid%20production%20by.pdf |
| _version_ | 1848844468635041792 |
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| author | Masoumian, M. Ariff, Arbakariya Ahmad, Syahida Mahmood, Maziah |
| author_facet | Masoumian, M. Ariff, Arbakariya Ahmad, Syahida Mahmood, Maziah |
| author_sort | Masoumian, M. |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensis treated with 2 mg/l of 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). To optimize the precursors to increase the production of flavonoid, different precursors were used. The data showed that 4 mg/l proline produced the highest flavonoid yield (10.77 ± 0.25 mg/g DW). The increase in proline concentration did not significantly increase the production of flavonoid. The highest flavonoid yield (10.59 ± 0.18 mg/g DW) was produced in 1 mg/l of glutamine. No significant increase was attained in the flavonoid yield in callus treated with 2, 3 mg/l compared with the control. Phenylalanine at the concentration of 3 mg/l, successfully triggered the production of flavonoid (11.43 ± 0.12 mg/g DW), which was 23% higher than the control. The highest flavonoid production was attained in calluses treated with 4 mg/l of naringenin; and it was 19.72% higher compared with the control. |
| first_indexed | 2025-11-15T08:31:24Z |
| format | Article |
| id | upm-22375 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T08:31:24Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| recordtype | eprints |
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| spelling | upm-223752017-11-15T03:11:28Z http://psasir.upm.edu.my/id/eprint/22375/ Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues Masoumian, M. Ariff, Arbakariya Ahmad, Syahida Mahmood, Maziah Callus tissue of Hydrocotyle bonariensis was initiated from the leaf of H. bonariensis treated with 2 mg/l of 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/l kinetin. The culture was kept at 25°C, under light (cool white fluorescent tubes, 1200 lux). To optimize the precursors to increase the production of flavonoid, different precursors were used. The data showed that 4 mg/l proline produced the highest flavonoid yield (10.77 ± 0.25 mg/g DW). The increase in proline concentration did not significantly increase the production of flavonoid. The highest flavonoid yield (10.59 ± 0.18 mg/g DW) was produced in 1 mg/l of glutamine. No significant increase was attained in the flavonoid yield in callus treated with 2, 3 mg/l compared with the control. Phenylalanine at the concentration of 3 mg/l, successfully triggered the production of flavonoid (11.43 ± 0.12 mg/g DW), which was 23% higher than the control. The highest flavonoid production was attained in calluses treated with 4 mg/l of naringenin; and it was 19.72% higher compared with the control. Academic Journals 2011 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/22375/1/Effect%20of%20precursors%20on%20flavonoid%20production%20by.pdf Masoumian, M. and Ariff, Arbakariya and Ahmad, Syahida and Mahmood, Maziah (2011) Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues. African Journal of Biotechnology, 10 (32). art. no. CB9F21230655. pp. 6021-6029. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/CB9F21230655 |
| spellingShingle | Masoumian, M. Ariff, Arbakariya Ahmad, Syahida Mahmood, Maziah Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues |
| title | Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues |
| title_full | Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues |
| title_fullStr | Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues |
| title_full_unstemmed | Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues |
| title_short | Effect of precursors on flavonoid production by Hydrocotyle bonariensis callus tissues |
| title_sort | effect of precursors on flavonoid production by hydrocotyle bonariensis callus tissues |
| url | http://psasir.upm.edu.my/id/eprint/22375/ http://psasir.upm.edu.my/id/eprint/22375/ http://psasir.upm.edu.my/id/eprint/22375/1/Effect%20of%20precursors%20on%20flavonoid%20production%20by.pdf |