Effect of medium composition and culture condition on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07, a strain isolated from budu

The effect of medium composition (type and concentration of carbon and nitrogen sources) and culture conditions (pH, aeration and temperature) on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07 was studied in shake flask culture. The highest BLIS produ...

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Main Authors: Abbasiliasi, Sahar, Ramanan, Ramakrishnan Nagasundara, Tengku Ibrahim, Tengku Azmi, Mustafa, Shuhaimi, Mohamad, Rosfarizan, Mohd Daud, Hassan, Ariff, Arbakariya
Format: Article
Published: Taylor & Francis 2011
Online Access:http://psasir.upm.edu.my/id/eprint/22373/
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author Abbasiliasi, Sahar
Ramanan, Ramakrishnan Nagasundara
Tengku Ibrahim, Tengku Azmi
Mustafa, Shuhaimi
Mohamad, Rosfarizan
Mohd Daud, Hassan
Ariff, Arbakariya
author_facet Abbasiliasi, Sahar
Ramanan, Ramakrishnan Nagasundara
Tengku Ibrahim, Tengku Azmi
Mustafa, Shuhaimi
Mohamad, Rosfarizan
Mohd Daud, Hassan
Ariff, Arbakariya
author_sort Abbasiliasi, Sahar
building UPM Institutional Repository
collection Online Access
description The effect of medium composition (type and concentration of carbon and nitrogen sources) and culture conditions (pH, aeration and temperature) on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07 was studied in shake flask culture. The highest BLIS production was obtained from de Man, Rogosa and Sharpe (MRS) broth, which was 4%, 22%, 51% and 63% higher than those obtained in M17 Broth, Tryptic Soy Broth (TSB), Brain Heart Infusion Broth (BHIB) and Nutrient Broth (NB) respectively. The addition of meat extract (5 g/L) and yeast extract (5 g/L) to MRS broth increased further BLIS production by 5% and 8%, respectively. The production of BLIS was further improved by 11% and 13% through the addition of tryptone (3 g/L) and glucose (10 g/L), respectively. The growth of Lb. paracasei LA07 and BLIS production were not significantly affected by agitation, either in anaerobic or in aerobic conditions. The optimum initial pH and temperature for BLIS production by Lb. paracasei LA07 was pH 8.5 and 30 °C, respectively.
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institution Universiti Putra Malaysia
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spelling upm-223732015-12-07T04:53:10Z http://psasir.upm.edu.my/id/eprint/22373/ Effect of medium composition and culture condition on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07, a strain isolated from budu Abbasiliasi, Sahar Ramanan, Ramakrishnan Nagasundara Tengku Ibrahim, Tengku Azmi Mustafa, Shuhaimi Mohamad, Rosfarizan Mohd Daud, Hassan Ariff, Arbakariya The effect of medium composition (type and concentration of carbon and nitrogen sources) and culture conditions (pH, aeration and temperature) on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07 was studied in shake flask culture. The highest BLIS production was obtained from de Man, Rogosa and Sharpe (MRS) broth, which was 4%, 22%, 51% and 63% higher than those obtained in M17 Broth, Tryptic Soy Broth (TSB), Brain Heart Infusion Broth (BHIB) and Nutrient Broth (NB) respectively. The addition of meat extract (5 g/L) and yeast extract (5 g/L) to MRS broth increased further BLIS production by 5% and 8%, respectively. The production of BLIS was further improved by 11% and 13% through the addition of tryptone (3 g/L) and glucose (10 g/L), respectively. The growth of Lb. paracasei LA07 and BLIS production were not significantly affected by agitation, either in anaerobic or in aerobic conditions. The optimum initial pH and temperature for BLIS production by Lb. paracasei LA07 was pH 8.5 and 30 °C, respectively. Taylor & Francis 2011 Article PeerReviewed Abbasiliasi, Sahar and Ramanan, Ramakrishnan Nagasundara and Tengku Ibrahim, Tengku Azmi and Mustafa, Shuhaimi and Mohamad, Rosfarizan and Mohd Daud, Hassan and Ariff, Arbakariya (2011) Effect of medium composition and culture condition on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07, a strain isolated from budu. Biotechnology and Biotechnological Equipment, 25 (4). pp. 2652-2657. ISSN 1310-2818; ESSN: 1314-3530 http://www.tandfonline.com/doi/abs/10.5504/BBEQ.2011.0101 10.5504/BBEQ.2011.0101
spellingShingle Abbasiliasi, Sahar
Ramanan, Ramakrishnan Nagasundara
Tengku Ibrahim, Tengku Azmi
Mustafa, Shuhaimi
Mohamad, Rosfarizan
Mohd Daud, Hassan
Ariff, Arbakariya
Effect of medium composition and culture condition on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07, a strain isolated from budu
title Effect of medium composition and culture condition on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07, a strain isolated from budu
title_full Effect of medium composition and culture condition on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07, a strain isolated from budu
title_fullStr Effect of medium composition and culture condition on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07, a strain isolated from budu
title_full_unstemmed Effect of medium composition and culture condition on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07, a strain isolated from budu
title_short Effect of medium composition and culture condition on the production of bacteriocin-like inhibitory substances (BLIS) by Lactobacillus paracasei LA07, a strain isolated from budu
title_sort effect of medium composition and culture condition on the production of bacteriocin-like inhibitory substances (blis) by lactobacillus paracasei la07, a strain isolated from budu
url http://psasir.upm.edu.my/id/eprint/22373/
http://psasir.upm.edu.my/id/eprint/22373/
http://psasir.upm.edu.my/id/eprint/22373/