Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzymes inhibition
The in vivo and in vitro mechanistic anti-inflammatory actions of cucurbitacin E (CE) (Citrullus lanatus var. citroides) were examined. The results showed that LPS/INF-γ increased NO production in RAW264.7 macrophages, whereas L-NAME and CE curtailed it. CE did not reveal any cytotoxicity on RAW264....
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
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Elsevier
2011
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| Online Access: | http://psasir.upm.edu.my/id/eprint/22371/ |
| _version_ | 1848844467413450752 |
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| author | Abdelwahab, Siddig Ibrahim Ahmed Hassan, Loiy Elsir Mohd Sirat, Hasnah Mohamed Ahmed Yagi, Sakina Mohamed, Waleed Sayed Koko Mohan, Syam Taha, Manal Mohamed Elhassan Ahmad, Syahida Cheah, Shiau Chuen Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa A. Hadi, A. Hamid |
| author_facet | Abdelwahab, Siddig Ibrahim Ahmed Hassan, Loiy Elsir Mohd Sirat, Hasnah Mohamed Ahmed Yagi, Sakina Mohamed, Waleed Sayed Koko Mohan, Syam Taha, Manal Mohamed Elhassan Ahmad, Syahida Cheah, Shiau Chuen Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa A. Hadi, A. Hamid |
| author_sort | Abdelwahab, Siddig Ibrahim |
| building | UPM Institutional Repository |
| collection | Online Access |
| description | The in vivo and in vitro mechanistic anti-inflammatory actions of cucurbitacin E (CE) (Citrullus lanatus var. citroides) were examined. The results showed that LPS/INF-γ increased NO production in RAW264.7 macrophages, whereas L-NAME and CE curtailed it. CE did not reveal any cytotoxicity on RAW264.7 and WRL-68 cells. CE inhibited both COX enzymes with more selectivity toward COX-2. Intraperitoneal injection of CE significantly suppressed carrageenan-induced rat's paw edema. ORAC and FRAP assays showed that CE is not a potent ROS scavenger. It could be concluded that CE is potentially useful in treating inflammation through the inhibition of COX and RNS but not ROS. |
| first_indexed | 2025-11-15T08:31:23Z |
| format | Article |
| id | upm-22371 |
| institution | Universiti Putra Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-15T08:31:23Z |
| publishDate | 2011 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | upm-223712015-12-07T04:47:12Z http://psasir.upm.edu.my/id/eprint/22371/ Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzymes inhibition Abdelwahab, Siddig Ibrahim Ahmed Hassan, Loiy Elsir Mohd Sirat, Hasnah Mohamed Ahmed Yagi, Sakina Mohamed, Waleed Sayed Koko Mohan, Syam Taha, Manal Mohamed Elhassan Ahmad, Syahida Cheah, Shiau Chuen Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa A. Hadi, A. Hamid The in vivo and in vitro mechanistic anti-inflammatory actions of cucurbitacin E (CE) (Citrullus lanatus var. citroides) were examined. The results showed that LPS/INF-γ increased NO production in RAW264.7 macrophages, whereas L-NAME and CE curtailed it. CE did not reveal any cytotoxicity on RAW264.7 and WRL-68 cells. CE inhibited both COX enzymes with more selectivity toward COX-2. Intraperitoneal injection of CE significantly suppressed carrageenan-induced rat's paw edema. ORAC and FRAP assays showed that CE is not a potent ROS scavenger. It could be concluded that CE is potentially useful in treating inflammation through the inhibition of COX and RNS but not ROS. Elsevier 2011-12 Article PeerReviewed Abdelwahab, Siddig Ibrahim and Ahmed Hassan, Loiy Elsir and Mohd Sirat, Hasnah and Mohamed Ahmed Yagi, Sakina and Mohamed, Waleed Sayed Koko and Mohan, Syam and Taha, Manal Mohamed Elhassan and Ahmad, Syahida and Cheah, Shiau Chuen and Mohd Fauzi, Putri Narrima and Rais, Mohd Mustafa and A. Hadi, A. Hamid (2011) Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzymes inhibition. Fitoterapia, 82 (8). pp. 1190-1197. ISSN 0367-326X; ESSN: 1873-6971 http://www.sciencedirect.com/science/article/pii/S0367326X11001870 10.1016/j.fitote.2011.08.002 |
| spellingShingle | Abdelwahab, Siddig Ibrahim Ahmed Hassan, Loiy Elsir Mohd Sirat, Hasnah Mohamed Ahmed Yagi, Sakina Mohamed, Waleed Sayed Koko Mohan, Syam Taha, Manal Mohamed Elhassan Ahmad, Syahida Cheah, Shiau Chuen Mohd Fauzi, Putri Narrima Rais, Mohd Mustafa A. Hadi, A. Hamid Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzymes inhibition |
| title | Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzymes inhibition |
| title_full | Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzymes inhibition |
| title_fullStr | Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzymes inhibition |
| title_full_unstemmed | Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzymes inhibition |
| title_short | Anti-inflammatory activities of cucurbitacin E isolated from Citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzymes inhibition |
| title_sort | anti-inflammatory activities of cucurbitacin e isolated from citrullus lanatus var. citroides: role of reactive nitrogen species and cyclooxygenase enzymes inhibition |
| url | http://psasir.upm.edu.my/id/eprint/22371/ http://psasir.upm.edu.my/id/eprint/22371/ http://psasir.upm.edu.my/id/eprint/22371/ |